| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82574-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-412SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80877 | |||
| BSA Free | NB110-81529SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-54155SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89557-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74678SS | |||
| Azide and BSA Free | NBP2-80876 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59320-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71363 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73394 | |||
![Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Analysis in human colon and liver tissues using NBP1-82574 antibody. Corresponding SLC9A3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP1-82574-img0023.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Analysis in human colon and liver tissues using NBP1-82574 antibody. Corresponding SLC9A3 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human colon, kidney, liver and small intestine using Anti-SLC9A3 antibody NBP1-82574 (A) shows similar protein distribution across tissues to independent antibody NBP1-82575 (B).](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP1-82574-img0025.jpg)
![Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human liver shows no membranous positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP1-82574-img0028.jpg)
![Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human colon shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP1-82574-img0024.jpg)
![Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human gallbladder shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP1-82574-img0026.jpg)
![Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human kidney shows weak positivity in apical membrane in cells in tubules.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP1-82574-img0027.jpg)
![Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP1-82574] - Staining of human small intestine shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP1-82574-img0029.jpg)
![Western Blot: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Analysis of 58K golgi protein expression in rat liver tissue using NB600-412.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-58K-9-Western-Blot-NB600-412-img0007.jpg "Western Blot: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Analysis of 58K golgi protein expression in rat liver tissue using NB600-412.")
![Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody (58K-9) [NB600-412] - HeLa cells, cultured on cover slips, were fixed with 10% formalin for 10 minutes and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were then incubated with 1:200 dilution of anti-58K Golgi Protein antibody (clone 58K-9) for overnight at 4C and detected with an anti-mouse Dylight 488 (Green) secondary at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at 1:500 dilution. Nuclei were counterstained with DAPI solution (Blue) [NBP2-31156]. Cells were imaged using a 40X objective. Antibody clone 58K-9 generated a specific signal in the Golgi apparatus of the cells.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-58K-9-Immunocytochemistry-Immunofluorescence-NB600-412-img0010.jpg)
![Immunohistochemistry: 58K Golgi Protein Antibody (58K-9) [NB600-412] - DMD muscle co-stained for Neu5Gc (green) with 58K Golgi, a Golgi marker, in red, and DAPI (blue). Arrow marks region of coincident staining (yellow) for Neu5Gc and 58K Golgi. Bar is 50 um. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0088226) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-58K-9-Immunohistochemistry-NB600-412-img0011.jpg)
![Immunohistochemistry: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Analysis of 58K Golgi Protein in mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-58K-9-Immunohistochemistry-NB600-412-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Assessing Golgi morphology observed apoptosis in mBECs using Mouse 58K Golgi Protein at a dilution of 1:100 overnight at 4 °C. This image was taken on a Zeiss LSM 710 Confocal Microscope (META) with Zeiss Plan Apo 63x/1.40 oil. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nb600-412_mouse-monoclonal-58k-golgi-protein-antibody-58k-9-immunocytochemistry-immunofluorescence-204202310243.jpg)
![Immunocytochemistry/ Immunofluorescence: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Co-localization of Neu5Gc staining with markers for endosomes & Golgi in BMD & DMD muscle.(A) BMD muscle co-stained for Neu5Gc (green) & clathrin (red), a marker of endosomes. Merged image on right shows overlap of Neu5Gc & clathrin expression in yellow. Arrow marks several examples of co-staining. (B) DMD muscle co-stained for Neu5Gc (green) with LAMP1, a lysosomal marker, 58K Golgi, a Golgi marker, or calnexin, & endoplasmic reticulum marker, all in red, & DAPI (blue). Arrow marks region of coincident staining (yellow) for Neu5Gc & 58K Golgi. Bar is 50 µm for all panels in A & B. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24505439), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-412_mouse-monoclonal-58k-golgi-protein-antibody-58k-9-310202415304219.jpg)
![Western Blot: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Detection of NHE3 [p Ser552] in human kidney lysate.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-p-Ser552-Antibody-14D5-Western-Blot-NB110-81529-img0005.jpg "Western Blot: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Detection of NHE3 [p Ser552] in human kidney lysate.")
![Immunohistochemistry: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Mouse intestine, image courtesy of verified customer review.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-p-Ser552-Antibody-14D5-Immunofluorescence-NB110-81529-img0008.jpg)
![Simple Western: NHE3/SLC9A3 [p Ser552] Antibody (14D5) [NB110-81529] - Simple Western lane view shows a specific band for NHE3 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-p-Ser552-Antibody-14D5-Simple-Western-NB110-81529-img0006.jpg)
![Western Blot: Lipase A Antibody [NBP1-54155] - Analysis using NBP1-54155 on cellular extracts of human prostate adenoma tissue, DU145, PC3, and 22RV1 prostate cancer cell lines, and HFL1 Normal primary human fibroblasts. NBP1-54155 revealed a strong specific band at the expected molecular weight for Lipase A at 45 kDa in different lysates of human cancer cell lines. NBP1-54155 specifically reveals LIPA in extracts from human prostate adenoma tissue and prostate cancer cell lines DU145, PC3, and 22RV1.The protein bands were visible in the positive but not in the negative controls. Therefore, NBP1-54155 was found to be suitable for checking the expression levels of Lipase A protein. Image from verified customer review.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Western-Blot-NBP1-54155-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: Lipase A Antibody [NBP1-54155] - Analysis using NBP1-54155 on cellular extracts of human prostate adenoma tissue, DU145, PC3, and 22RV1 prostate cancer cell lines, and HFL1 Normal primary human fibroblasts. NBP1-54155 revealed a strong specific band at the expected molecular weight for Lipase A at 45 kDa in different lysates of human cancer cell lines. NBP1-54155 specifically reveals LIPA in extracts from human prostate adenoma tissue and prostate cancer cell lines DU145, PC3, and 22RV1.The protein bands were visible in the positive but not in the negative controls. Therefore, NBP1-54155 was found to be suitable for checking the expression levels of Lipase A protein. Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Western Blot: Lipase A Antibody [NBP1-54155] - NR and Metf promote FoxO1-mediated Lipa upregulation in visceral AT of adult mice. Adult C57/BL6 mice (5 months) were nutrient restricted (NR) by 24 h fasting or treated for 10 days with Metf (400 mg/kg) dissolved in drinking water (n=4 mice per group). Western blot of FoxO1 and Lipa in total protein extracts of explanted visceral (epididymal) AT. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2013404) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Western-Blot-NBP1-54155-img0009.jpg)
![Simple Western: Lipase A Antibody [NBP1-54155] - Image shows a specific band for Lipase A in 0.5 mg/mL of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Simple-Western-NBP1-54155-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Lipase A Antibody [NBP1-54155] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-Lipase A Antibody NBP1-54155 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54155-img0010.jpg)
![Immunohistochemistry-Paraffin: Lipase A Antibody - BSA Free [NBP1-54155] - Analysis of a FFPE tissue section of human stomach using 1:200 dilution of Lipase A antibody (NBP1-54155). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/Lipase-A-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-54155-img0011.jpg)
![Western Blot: Lipase A Antibody [NBP1-54155] - Detection of LIPA in HepG2 whole cell extract.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Western-Blot-NBP1-54155-img0001.jpg)
![Immunohistochemistry: Lipase A Antibody [NBP1-54155] - Staining of LIPA in mouse stomach smooth muscle.](http://images.novusbio.com/fullsize/Lipase-A-Antibody-Immunohistochemistry-NBP1-54155-img0005.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416154610.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416165629.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - AMPK drives Lipa-released FFAs oxidation restraining energetic catastrophe. (a) 3T3-L1 cells were transfected with DN-AMPK or empty vector. RT-qPCR analysis of relative peroxisome proliferator-activated receptor gamma-1 alpha , peroxisome proliferator-activated receptor-alpha , carnitine palmitoyltransferase 1b & acyl-CoA oxidase 1 mRNA levels were performed after 4 h of NR or 16 h of Metf treatment. Dashed line indicates the mRNA value of untreated DN-AMPK cells (Ctr). mRNA levels of untreated cells transfected with empty vector were similar to untreated DN-AMPK cells (data not shown). (b) Cheminoluminescent assay of ATP level in 3T3-L1 adipocytes transfected with DN-AMPK or empty vector after 8 h NR or 16 h Metf treatment. ATP level was expressed as pmol ATP per mg protein. (c) After 8 h of NR or 16 h Metf treatment, FFAs were enzymatically detected in culture medium of 3T3-L1 adipocytes transfected with DN-AMPK or empty vector. Values were expressed as μg FFAs per mg protein. (d) Left panel: western blot of AMPKpT172, PARP-1 & cleaved form of caspase-3 in 3T3-L1 adipocytes transfected with DN-AMPK or empty vector & subjected to 8 h NR. Right panel: cytofluorimetric analysis of apoptosis in DN-AMPK cells subjected to 8 h NR. (e) Western blot of PARP-1 & cleaved form of caspase-3 in 3T3-L1 adipocytes transfected with DN-AMPK or empty vector & treated with Metf for 16 h. (f) Western blot of FoxO1, Lipa, LC3 in 3T3-L1 adipocytes transfected with DN-AMPK or empty vector & subjected to 4 h NR. beta -actin was used as loading control. All values are given as mean±S.D. *P<0.05, **P<0.01 versus controls; °P<0.05, °°P<0.01 versus Metf treatment. All data are representative of at least three independent experiments Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-31020241618936.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416171493.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416171436.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - Lipa downregulation impairs lipid breakdown & elicits cell death in nutrient restricted adipocytes. (a) 3T3-L1 adipocytes were transfected with siRNA against Lipa (Lipa(−)) or with a scramble siRNA (Scr). Western blot of Lipa, PARP-1 & cleaved form of caspase-3 in total protein extracts from 3T3-L1 adipocytes after 4 h of NR. (b) TG content was quantified by ORO staining in fixed 3T3-L1 adipocytes 6 h after NR. (c) RT-qPCR analysis of relative peroxisome proliferator-activated receptor gamma-1 alpha , peroxisome proliferator-activated receptor-alpha & carnitine palmitoyltransferase 1b mRNA levels was performed in 3T3-L1 adipocytes 4 h after NR. (d) FFAs were analyzed in culture medium 6 h after NR. beta -actin was used as loading control. All values are given as mean±S.D. *P<0.05, **P<0.01 versus controls; °P<0.05 versus NR treatment. All data are representative of at least three independent experiments Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-310202416163713.jpg)
![Western Blot: Lipase A Antibody - BSA Free [NBP1-54155] - FoxO1-mediated lysosomal acid lipase (Lipa) induction in NR & Metf-treated 3T3-L1 adipocytes. (a) WB of FoxO1, ATGL & Lipa in total protein extracts from 3T3-L1 adipocytes at different times of NR. (b) RT-qPCR analysis of relative Lipa & ATGL mRNA levels in 3T3-L1 after 4 h from NR. Dashed line indicates mRNA value of controls. (c) After 4 h from NR, 3T3-L1 adipocytes refed w/ complete cell culture medium (CM) up to 8 h. Total protein extracts used for WB analysis of FoxO1 & Lipa. (d) WB of FoxO1 in total & nuclear protein extracts from 3T3-L1 adipocytes at different times of NR. (e) ChIP assay carried out on crosslinked nuclei from 3T3-L1 adipocytes subjected to NR for 4 h & Metf for 16 h by using FoxO1 antibody followed by qPCR analysis of FoxO1RE on Lipa promoter (−51 bp). Dashed line indicates IgG value. (f & g) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa (f) & RT-qPCR analysis of relative Lipa mRNA level (g) performed in 3T3-L1 adipocytes 4 h after NR. (h) WB of FoxO1 & Lipa in 3T3-L1 adipocytes at different times of 5 mM Metformin (Metf) treatment. (i) Confocal analysis of FoxO1 localization in 3T3-L1 adipocytes treated w/ 5 mM Metf for 16 h. Nuclei stained w/ Hoechst 33342. Colocalization plugin (ImageJ Software) used to identify FoxO1-Hoechst colocalization (white spots). (j) RT-qPCR analysis of relative Lipa mRNA level performed in 3T3-L1 adipocytes treated w/ Metf for 16 h. (k) 3T3-L1 adipocytes transfected w/ siRNA against FoxO1 (FoxO1(−)) or w/ a scramble siRNA (Scr). WB of FoxO1 & Lipa performed in 3T3-L1 adipocytes treated w/ 5 mM Metf for 24 h. All values given as mean±S.D. (n=4). *P<0.05, **P<0.01 versus controls. °P<0.05 versus NR Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24136225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-54155_rabbit-polyclonal-lipase-a-antibody-31020241616083.jpg)
![Western Blot: CPT1B Antibody [NBP1-59576] - Sample Type : 1: 45ug human capan1 cell lysate Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti-rabbit HRP Secondary Antibody Dilution : 1:5000 Color](http://images.novusbio.com/fullsize/CPT1B-Antibody-Western-Blot-NBP1-59576-img0007.jpg "Western Blot: CPT1B Antibody [NBP1-59576] - Sample Type : 1: 45ug human capan1 cell lysate Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti-rabbit HRP Secondary Antibody Dilution : 1:5000 Color")
![Immunohistochemistry: CPT1B Antibody [NBP1-59576] - searcher: Dr. Pankaj Kumar Singh, UNMC, Omaha, NE Application: IHC Species+tissue/cell type:Species+tissue/cell type: Human Capan1 cells Primary antibody dilution: 1:300 Secondary antibody: Anti-rabbit Alexa Fluor 488 Secondary antibody dilution:1:200](http://images.novusbio.com/fullsize/CPT1B-Antibody-Immunohistochemistry-NBP1-59576-img0004.jpg)
![Western Blot: CPT1B Antibody [NBP1-59576] - Titration: 0.2-1 ug/ml, Positive Control: HT1080 cell lysate.](http://images.novusbio.com/fullsize/CPT1B-Antibody-Western-Blot-NBP1-59576-img0003.jpg)
![Western Blot: CPT1B Antibody [NBP1-59576] - Sample Tissue: Human 293T Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/CPT1B-Antibody-Western-Blot-NBP1-59576-img0006.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP1-89557-img0015.jpg "Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP1-89557-img0012.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP1-89557-img0013.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody [NBP1-89557] - Staining of human pancreas shows moderate granular cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/CLPP-Antibody-Immunohistochemistry-Paraffin-NBP1-89557-img0014.jpg)
![Western Blot: NHE3/SLC9A3 [p Ser605] Antibody (10A8) [NB110-74678] - Detection of phospho Ser605 in transfected COS-7 cells using NB110-74678. A. untreated B. forskolin and IBMX treated.](http://images.novusbio.com/fullsize/NHE3-SLC9A3-p-Ser605-Antibody-10A8-Western-Blot-NB110-74678-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: NHE3/SLC9A3 [p Ser605] Antibody (10A8) [NB110-74678] - Detection of phospho Ser605 in transfected COS-7 cells using NB110-74678. A. untreated B. forskolin and IBMX treated.")
![Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protein using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (NBP2-59320). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (NBP2-59320) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Western-Blot-NBP2-59320-img0007.jpg "Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa SUR1 protein using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (NBP2-59320). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (NBP2-59320) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~160 kDa.")
![Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16 (NBP2-59320). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody (NBP2-59320) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SUR1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Immunocytochemistry-Immunofluorescence-NBP2-59320-img0008.jpg)
![Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis of mouse pancreatic islets. Lane 1: Molecular Weight Ladder. Lanes 2-5: Samples from pooled pancreatic islets. Load: ~10ug. Primary Antibody: Anti-SUR1 Monoclonal Antibody at 1:200 for ~16hrs at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1hr RT. Image from verified customer review.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Western-Blot-NBP2-59320-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis using Mouse Anti-SUR1 Monoclonal Antibody, Clone S289-16. Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SUR1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) SUR1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Immunocytochemistry-Immunofluorescence-NBP2-59320-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Glial fibrillary acidic protein (GFAP)-positive specimens from human contusion- traumatic brain injury (TBI) exhibit sulfonylurea receptor 1 (SUR1) expression in astrocytes.Double immunolabeling for SUR1 (red) and GFAP (green) showed astrocyte expression of SUR1; merged images confirm co-localization (yellow); in situ hybridization of the same tissue section for Abcc8 messenger RNA showed positive signal co-localized with GFAP-positive, SUR1-expressing astrocytes; arrowheads point to cells with all three signals. Image collected and cropped by CiteAb from the following publication (//www.liebertpub.com/doi/10.1089/neu.2018.5986) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SUR1-Antibody-S289-16-Immunocytochemistry-Immunofluorescence-NBP2-59320-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Glial fibrillary acidic protein (GFAP)–positive specimens from human contusion- traumatic brain injury (TBI) exhibit KIR6.2 expression in astrocytes. (A) Immunolabeling for KIR6.2 showed sparse immunoreactivity in the control specimen (CTR) vs. widespread expression in a GFAP-positive specimen from contusion-TBI. (B) Double immunolabeling for GFAP (red) & KIR6.2 (green) showed astrocyte expression of KIR6.2; merged images confirm co-localization (yellow); in situ hybridization of the same tissue section for Kcnj11 messenger RNA showed positive signal co-localized with GFAP-positive, KIR6.2-expressing astrocytes; arrowheads point to cells with all three signals. (C) Double immunolabeling showed that KIR6.2 (red) & sulfonylurea receptor 1 (SUR1; green) were co-localized (yellow) in astrocytes. (D) ImmunoFRET for SUR1 (magenta) & KIR6.2 (red) showed co-assembly of SUR1-KIR6.2 heteromers (yellow pseudocolor) in astrocytes. (E) Double immunolabeling showed that KIR6.2 (green) & transient receptor potential cation channel subfamily M member 4 (TRPM4) (red) were co-localized in astrocytes. The findings illustrated are representative of all GFAP-positive specimens from eight cases of human contusion-TBI. (case #2, 11 days post-TBI; case #5, 1 day post-TBI) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30160201), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-59320_mouse-monoclonal-sur1-antibody-s289-16-310202416114773.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (OTI1F6) [NBP2-03323] - CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CLPP mouse monoclonal antibody.CLPP Antibody (OTI1F6)](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunohistochemistry-Paraffin-NBP2-03323-img0005.jpg "Immunohistochemistry-Paraffin: CLPP Antibody (OTI1F6) [NBP2-03323] - CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CLPP mouse monoclonal antibody.CLPP Antibody (OTI1F6)")
![Western Blot: CLPP Antibody (1F6) [NBP2-03323] Analysis of extracts (35ug) from 9 different cell lines by using anti-CLPP monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Western-Blot-NBP2-03323-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: CLPP Antibody (1F6) [NBP2-03323] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CLPP.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunocytochemistry-Immunofluorescence-NBP2-03323-img0010.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Human liver tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunohistochemistry-Paraffin-NBP2-03323-img0009.jpg)
![Flow Cytometry: CLPP Antibody (1F6) [NBP2-03323] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CLPP antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Flow-Cytometry-NBP2-03323-img0001.jpg)
![Western Blot: CLPP Antibody (1F6) [NBP2-03323] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLPP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLPP.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Western-Blot-NBP2-03323-img0002.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunohistochemistry-Paraffin-NBP2-03323-img0003.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunohistochemistry-Paraffin-NBP2-03323-img0004.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunohistochemistry-Paraffin-NBP2-03323-img0006.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunohistochemistry-Paraffin-NBP2-03323-img0007.jpg)
![Immunohistochemistry-Paraffin: CLPP Antibody (1F6) [NBP2-03323] - Staining of paraffin-embedded Human Kidney tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-1F6-Immunohistochemistry-Paraffin-NBP2-03323-img0008.jpg)
![Western Blot: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLPP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLPP.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Western-Blot-NBP2-71363-img0002.jpg "Western Blot: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLPP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLPP.")
![Immunocytochemistry/Immunofluorescence: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CLPP.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71363-img0004.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Human Kidney tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0010.jpg)
![Flow Cytometry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-CLPP antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71363-img0003.jpg)
![Western Blot: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Analysis of extracts (35ug) from 9 different cell lines by using anti-CLPP monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Western-Blot-NBP2-71363-img0001.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0005.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0006.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0007.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0008.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0009.jpg)
![Immunohistochemistry: CLPP Antibody (OTI1F6) - Azide and BSA Free [NBP2-71363] - Staining of paraffin-embedded Human liver tissue using anti-CLPP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CLPP-Antibody-OTI1F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71363-img0011.jpg)
![Western Blot: IPMK Antibody [NBP1-32250] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK antibody [N1C2] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IPMK-Antibody-Western-Blot-NBP1-32250-img0010.jpg "Western Blot: IPMK Antibody [NBP1-32250] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK antibody [N1C2] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: IPMK Antibody [NBP1-32250] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/IPMK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32250-img0004.jpg)
![Immunohistochemistry-Paraffin: IPMK Antibody [NBP1-32250] - Mouse intestine. IPMK stained by IPMK antibody [N1C2] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/IPMK-Antibody-Immunohistochemistry-Paraffin-NBP1-32250-img0011.jpg)
![Western Blot: IPMK Antibody [NBP1-32250] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IPMK-Antibody-Western-Blot-NBP1-32250-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: IPMK Antibody [NBP1-32250] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK antibody [N1C2] diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32250_rabbit-polyclonal-ipmk-antibody-1610202419414528.jpg)
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK antibody [N1C2] (NBP1-32250) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32250_rabbit-polyclonal-ipmk-antibody-western-blot-1411202518424118.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK antibody [N1C2] (NBP1-32250) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32250_rabbit-polyclonal-ipmk-antibody-western-blot-1411202518412924.jpg)
![Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Blot showing the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as expected, and no significant increase in the purine enzymes was detected between normoxic (21% oxygen) and hypoxic (1% oxygen) growth conditions. The positions of molecular markers surrounding each band of interest are shown for each blot. ADSL (NBP2-03107), ATIC (NBP2-01941), FGAMS (NBP1-84691), GART (H00002618-M01), HIF-1a (NB100-449), PAICS (NBP2-02817), PPAT (NBP2-02056). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32439803/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Western-Blot-NBP2-02056-img0026.jpg "Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Blot showing the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as expected, and no significant increase in the purine enzymes was detected between normoxic (21% oxygen) and hypoxic (1% oxygen) growth conditions. The positions of molecular markers surrounding each band of interest are shown for each blot. ADSL (NBP2-03107), ATIC (NBP2-01941), FGAMS (NBP1-84691), GART (H00002618-M01), HIF-1a (NB100-449), PAICS (NBP2-02817), PPAT (NBP2-02056). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32439803/) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Staining of paraffin-embedded Human liver tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Immunohistochemistry-Paraffin-NBP2-02056-img0023.jpg)
![Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Phosphoribosyl Pyrophosphate Amidotransferase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Western-Blot-NBP2-02056-img0013.jpg)
![Western Blot: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Phosphoribosyl Pyrophosphate Amidotransferase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Phosphoribosyl Pyrophosphate Amidotransferase.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Western-Blot-NBP2-02056-img0025.jpg)
![Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Immunohistochemistry-Paraffin-NBP2-02056-img0014.jpg)
![Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Immunohistochemistry-Paraffin-NBP2-02056-img0015.jpg)
![Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Immunohistochemistry-Paraffin-NBP2-02056-img0018.jpg)
![Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Staining of paraffin-embedded Human colon tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Immunohistochemistry-Paraffin-NBP2-02056-img0021.jpg)
![Immunohistochemistry-Paraffin: Phosphoribosyl Pyrophosphate Amidotransferase Antibody (OTI1B8) [NBP2-02056] - Staining of paraffin-embedded Human Kidney tissue using anti-Phosphoribosyl Pyrophosphate Amidotransferase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Phosphoribosyl-Pyrophosphate-Amidotransferase-Antibody-OTI1B8-Immunohistochemistry-Paraffin-NBP2-02056-img0022.jpg)