NHE3/SLC9A3 [p Ser605] Antibody (10A8) [PE]

• Reactivity: Mu, Rt, Po, Ma-Op, Pm, Rb
• Applications: WB, ICC/IF, IP
• Clone: 10A8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE

NHE3/SLC9A3 [p Ser605] Antibody (10A8) [PE] Summary

• Immunogen: Synthetic peptide made to a region surrounding the Serine 605 portion of rat NHE3. [UniProt# P26433]
• Modification: p Ser605
• Localization: Membrane.
• Specificity: This antibody detects NHE3 posphorylated at serine 605, and does not cross-react with the non-phosphopeptide.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC9A3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot
• Application Notes: This NHE3 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In WB a band can be seen at ~85 kDa.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 85 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse, rat, rabbit, opossum and primate. Porcine reactivity reported in scientific literature (PMID: 29510506).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for NHE3/SLC9A3 [p Ser605] Antibody (10A8) [PE]

• isoform 3
• MGC126718
• NHE3
• NHE3MGC126720
• SLC9A3
• solute carrier family 9 (sodium/hydrogen exchanger), member 3
• Solute carrier family 9 member 3

Background

NHE3 is a member of the Sodium/Hydrogen exchanger family that plays a crucial role in acid-base and volume homeostasis by mediating the majority of sodium and bicarbonate reabsorption in the proximal tubule of the kidney. Two PKA consensus sites have been discovered in rat NHE3, serine 552 and serine 605. Research suggests that dopamine-induced phosphorylation of these consensus sites inhibit NHE3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC9A3