IPMK Antibody - BSA Free

Western Blot: IPMK Antibody [NBP1-32250] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK antibody [N1C2] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: IPMK Antibody [NBP1-32250] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: IPMK Antibody [NBP1-32250] - Mouse intestine. IPMK stained by IPMK antibody [N1C2] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: IPMK Antibody [NBP1-32250] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: IPMK Antibody [NBP1-32250] -Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody.

Western Blot: IPMK Antibody [NBP1-32250] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IPMK antibody [N1C2] diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

IPMK Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit IPMK Antibody - BSA Free (NBP1-32250) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-IPMK Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human IPMK. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Rat (90%), Bovine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IPMK
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for IPMK Antibody - BSA Free

• EC 2.7.1.151
• IMPK
• Inositol 13,4,6-tetrakisphosphate 5-kinase
• inositol polyphosphate multikinase

Background

Inositol phosphate kinase with a broad substrate specificity. Has a preference forinositol-1,4,5-trisphosphate (Ins(1,4,5)P3) and inositol 1,3,4,6-tetrakisphosphate (Ins(1,3,4,6)P4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for IPMK Antibody (NBP1-32250)(3)

Publications using NBP1-32250

• Hong S, Kim K, Shim YR et al. A non-catalytic role of IPMK is required for PLC gamma 1 activation in T cell receptor signaling by stabilizing the PLC gamma 1-Sam68 complex. Cell communication and signaling : CCS 2024-10-31 [PMID: 39478550]
• Ik-Rak Jung, Rexford S. Ahima, Sangwon F. Kim, Giovanni Tarantino Time-Restricted Feeding Ameliorates Methionine–Choline Deficient Diet-Induced Steatohepatitis in Mice International Journal of Molecular Sciences 2024-01-23 [PMID: 38338668]
• Tyagi R, Chakraborty S, Tripathi S et al. Inositol polyphosphate multikinase modulates redox signaling through nuclear factor erythroid 2-related factor 2 and glutathione metabolism iScience 2023-07-21 [PMID: 37456841] (WB, KO)

Bioinformatics

• Gene Symbol: IPMK