NHE3/SLC9A3 [p Ser605] Antibody (10A8)


Western Blot: NHE3 [p Ser605] Antibody (10A8) [NB110-74678] - Detection of phospho Ser605 in transfected COS-7 cells using NB110-74678. A. untreated B. forskolin and IBMX treated

Product Details

Reactivity Mu, Rt, Op, Pm, RbSpecies Glossary
Applications WB, ICC/IF, IP
2.1 mg/ml

NHE3/SLC9A3 [p Ser605] Antibody (10A8) Summary

Synthetic peptide made to a region surrounding the Serine 605 portion of rat NHE3. [UniProt# P26433]
p Ser605
This antibody detects NHE3 posphorylated at serine 605, and does not cross-react with the non-phosphopeptide.
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
2.1 mg/ml
Protein G purified


Application Notes
This NHE3 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In WB a band can be seen at ~85 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
85 kDa
Read Publications using
NB110-74678 in the following applications:

  • WB
    2 publications

Reactivity Notes

Mouse, rat, rabbit, opossum and primate.

Alternate Names for NHE3/SLC9A3 [p Ser605] Antibody (10A8)

  • isoform 3
  • MGC126718
  • NHE3MGC126720
  • SLC9A3
  • solute carrier family 9 (sodium/hydrogen exchanger), member 3
  • Solute carrier family 9 member 3


Na+/H+ exchanger (NHE) family members are integral membrane proteins which play key role in catalyzing the extrusion of intracellular proton (H+) ions in exchange for extracellular sodium (Na+) ions for regulation of cellular pH as well as transepithelial ion/water transport. NHE3 or SLC9A3 is one of the best characterized NHEs that is a major proton extruding system driven by inward Na+ ion chemical gradient and is implicated in pH regulation to eliminate acids generated during metabolism or to tackle any potential adverse conditions in tissue microenvironment. NHE3 exists as a multi-pass membrane protein in cell membranes and is expressed most abundantly in colon and small intestine, followed by kidney and stomach, as well as in ventrolateral region of the medulla oblongata. In intestinal epithelial cells, it localizes to the ileal brush border and SGK1 mediated phosphorylation at Ser-661 has been shown to be associated with its enhanced cell membrane localization. NHE3 binds to SLC9A3R1 and SLC9A3R2, and interacts with CHP1, CHP2, SHANK2 PDZD3 and (interactions may decrease in response to elevated calcium ion levels). NHE3 is regulated by several hormones, neurotransmitters, and associated signaling systems such as cAMP, cGMP, and elevated intracellular calcium via mechanisms that are largely partially understood. NHE3 knock-out mice suffers from chronic diarrhea and altered salt as well as water homeostasis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: IHC, IHC-P
Species: Hu
Applications: IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, S-ELISA
Species: Hu
Applications: WB, IHC
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, IHC, ICC
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Mu, Rt, Op, Pm, Rb
Applications: WB, ICC/IF, IP

Publications for NHE3/SLC9A3 Antibody (NB110-74678)(3)

Reviews for NHE3/SLC9A3 Antibody (NB110-74678) (0)

There are no reviews for NHE3/SLC9A3 Antibody (NB110-74678). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

View specific protocols for NHE3/SLC9A3 Antibody (NB110-74678): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NHE3/SLC9A3 Antibody (NB110-74678) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB110-74678AF405
Alexa Fluor (R) 488 Labeled NB110-74678AF488
Alexa Fluor (R) 647 Labeled NB110-74678AF647
Alexa Fluor (R) 700 Labeled NB110-74678AF700
Allophycocyanin Labeled NB110-74678APC
Biotin Labeled NB110-74678B
DyLight 350 Labeled NB110-74678UV
DyLight 405 Labeled NB110-74678V
DyLight 405LS Labeled NB110-74678V3
DyLight 488 Labeled NB110-74678G
DyLight 550 Labeled NB110-74678R
DyLight 650 Labeled NB110-74678C
DyLight 680 Labeled NB110-74678FR
DyLight 755 Labeled NB110-74678IR
FITC Labeled NB110-74678F
HRP Labeled NB110-74678H
PE Labeled NB110-74678PE
PerCP Labeled NB110-74678PCP

Additional NHE3/SLC9A3 [p Ser605] Antibody (10A8) Products

Related Products by Gene

Bioinformatics Tool for NHE3/SLC9A3 Antibody (NB110-74678)

Discover related pathways, diseases and genes to NHE3/SLC9A3 Antibody (NB110-74678). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NHE3/SLC9A3 Antibody (NB110-74678)

Discover more about diseases related to NHE3/SLC9A3 Antibody (NB110-74678).

Pathways for NHE3/SLC9A3 Antibody (NB110-74678)

View related products by pathway.

PTMs for NHE3/SLC9A3 Antibody (NB110-74678)

Learn more about PTMs related to NHE3/SLC9A3 Antibody (NB110-74678).

Research Areas for NHE3/SLC9A3 Antibody (NB110-74678)

Find related products by research area.

Blogs on NHE3/SLC9A3

There are no specific blogs for NHE3/SLC9A3, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol SLC9A3

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB110-74678 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought