| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49529SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87695-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-157SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89284-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52979SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-55244SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54792SS | |||
| Azide and BSA Free | NBP2-80556 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-117SS | |||
![Western Blot: LXR alpha/NR1H3 Antibody [NB300-612] - LXRs are expressed in peripheral blood cells. LXR alpha/NR1H3 protein levels in protein extracts from PBMCs from these same donors were detected by Western blotting using rabbit anti-human LXR alpha/NR1H3 polyclonal antisera. Image collected and cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-6-59), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Western-Blot-NB300-612-img0013.jpg "Western Blot: LXR alpha/NR1H3 Antibody [NB300-612] - LXRs are expressed in peripheral blood cells. LXR alpha/NR1H3 protein levels in protein extracts from PBMCs from these same donors were detected by Western blotting using rabbit anti-human LXR alpha/NR1H3 polyclonal antisera. Image collected and cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-6-59), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: LXR alpha/NR1H3 Antibody [NB300-612] - Analysis of LXR alpha (green) showing staining in the nucleus of Hela cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Immunocytochemistry-Immunofluorescence-NB300-612-img0006.jpg)
![Flow Cytometry: LXR alpha/NR1H3 Antibody [NB300-612] - analysis of LXR alpha was done on HeLa. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton X-100 for 20 minutes, and blocked with 2.5% BSA for 30 minutes at room temperature. Cells were labeled with LXR alpha Rabbit Polyclonal Antibody (PA1330, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Flow-Cytometry-NB300-612-img0012.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody [NB300-612] - analysis was performed on nuclear enriched extracts (30 ug lysate) of MCF7 (Lane 1), THP-1 (Lane 2), A549 (Lane 3), SH-SY5Y (Lane 4), HL-60 (Lane 5) and KARPAS 299 (Lane 6). The blot was probed with Rabbit Anti-LXR alpha Polyclonal Antibody (2ug/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (0.25 ug/ml, 1:4000 dilution). A 50 kDa band corresponding to LXR alpha was observed across the cell lines tested. Known quantity of protein samples was electrophoresed using 4-12 % Bis-Tris gel, Electrophoresis System and Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with Blot 2 Dry Blotting System. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using ECL Chemiluminescent Substrate Reagent Kit.](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Western-Blot-NB300-612-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: LXR alpha/NR1H3 Antibody [NB300-612] - Analysis of LXR alpha (green) showing staining in the nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/LXR-alpha-NR1H3-Antibody-Immunocytochemistry-Immunofluorescence-NB300-612-img0005.jpg)
![Western Blot: ApoE4 Antibody (4E4) [NBP1-49529] - Analysis of ApoE4 expression in concentrated supernatants of CHO cells secreting human ApoE2, ApoE3 or ApoE4 using NBP1-49529. Image courtesy of Dr. Marko Roblek from the Medical University of Vienna.](http://images.novusbio.com/fullsize/ApoE4-Antibody-4E4-Western-Blot-NBP1-49529-img0004.jpg "Western Blot: ApoE4 Antibody (4E4) [NBP1-49529] - Analysis of ApoE4 expression in concentrated supernatants of CHO cells secreting human ApoE2, ApoE3 or ApoE4 using NBP1-49529. Image courtesy of Dr. Marko Roblek from the Medical University of Vienna.")
![Immunocytochemistry/Immunofluorescence: ApoE4 Antibody (4E4) [NBP1-49529] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ApoE4 (4E4) at 5 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ApoE4-Antibody-4E4-Immunocytochemistry-Immunofluorescence-NBP1-49529-img0005.jpg)
![Immunohistochemistry-Paraffin: FABP1/L-FABP Antibody [NBP1-87695] - Analysis in human liver and pancreas tissues. Corresponding FABP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-87695-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FABP1/L-FABP Antibody [NBP1-87695] - Analysis in human liver and pancreas tissues. Corresponding FABP1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: FABP1/L-FABP Antibody [NBP1-87695] - Western blot analysis in mouse liver tissue and rat liver tissue.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-Western-Blot-NBP1-87695-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: FABP1/L-FABP Antibody [NBP1-87695] - Staining of human cell line CACO-2 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87695-img0010.jpg)
![Immunohistochemistry-Paraffin: FABP1/L-FABP Antibody [NBP1-87695] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-87695-img0020.jpg)
![Western Blot: FABP1/L-FABP Antibody [NBP1-87695] - Analysis in human cell line HepG2.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-Western-Blot-NBP1-87695-img0006.jpg)
![Immunohistochemistry-Paraffin: FABP1/L-FABP Antibody [NBP1-87695] - Staining of human pancreas shows no positivity in exocrine glandular cells and islets of Langerhans as expected.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-87695-img0015.jpg)
![Immunohistochemistry-Paraffin: FABP1/L-FABP Antibody [NBP1-87695] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-87695-img0016.jpg)
![Immunohistochemistry-Paraffin: FABP1/L-FABP Antibody [NBP1-87695] - Staining of human kidney shows weak cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-Immunohistochemistry-Paraffin-NBP1-87695-img0019.jpg)
![Western Blot: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - (0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Proprotein-Convertase-9-PCSK9-Antibody-Western-Blot-NB300-959-img0005.jpg "Western Blot: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - (0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - (5ug/ml) staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/NB300-959_Immunohistochemistry-Paraffin-Proprotein-Convertase-9-PCSK9-Antibody-NB300-959-5ug-ml-staining-of-paraffin-embedded-Human-Liver-Heat-induced-antigen-retrieval-with-citrate-buffer-pH-6-HRP-staining--29320239254023.jpg)
![Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.](http://images.novusbio.com/fullsize/NB300-959_Immunohistochemistry-Paraffin-Proprotein-Convertase-9-PCSK9-Antibody-NB300-959-Negative-Control-showing-staining-of-paraffin-embedded-Human-Liver-with-no-primary-antibody--2932023925404.jpg)
![Western Blot: LXR alpha/NR1H3 Antibody [NB400-157] - WB detection of LXR in human liver.](http://images.novusbio.com/fullsize/LXR-Antibody-Western-Blot-NB400-157-img0001.jpg "Western Blot: LXR alpha/NR1H3 Antibody [NB400-157] - WB detection of LXR in human liver.")
![Simple Western: LXR alpha/NR1H3 Antibody [NB400-157] - Simple Western lane view shows a ~49 kDa for LXR in human skeletal muscle tissue lysate under reducing conditions. Image from verified customer review.](http://images.novusbio.com/fullsize/LXR-Antibody-Simple-Western-NB400-157-img0002.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar protein distribution across tissues to independent antibody NBP1-89285 (B).](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0009.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum, kidney, liver and testis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar protein distribution across tissues to independent antibody NBP1-89285 (B).")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: ACAT1 Antibody [NBP1-89284] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ACAT1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ACAT1 Antibody [NBP1-89284] - Analysis using Anti-ACAT1 antibody NBP1-89284 (A) shows similar pattern to independent antibody NBP1-89285 (B).](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-89284] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89284-img0007.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human cerebellum shows moderate positivity in mitochondria in Purkinje cells.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0014.jpg)
![Western Blot: ACAT1 Antibody [NBP1-89284] - ACAT1 porcine cerebral cortex. Image from verified customer review.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Western-Blot-NBP1-89284-img0008.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human testis shows strong positivity in mitochondria in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0011.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human kidney shows strong positivity in mitochondria in cells in tubules.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0012.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89284] - Staining of human liver shows strong positivity in mitochondria in hepatocytes.](http://images.novusbio.com/fullsize/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89284-img0013.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein A-I/ApoA1 Antibody [NBP2-15429] - Human ovarian cancer. Apolipoprotein A1 stained by Apolipoprotein A1 antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-Immunohistochemistry-Paraffin-NBP2-15429-img0011.jpg "Immunohistochemistry-Paraffin: Apolipoprotein A-I/ApoA1 Antibody [NBP2-15429] - Human ovarian cancer. Apolipoprotein A1 stained by Apolipoprotein A1 antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunocytochemistry/Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody [NBP2-15429] -HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Apolipoprotein A1 stained by Apolipoprotein A1 antibody diluted at 1:500.Blue: Fluoroshield with DAPI. Scale bar= 10um.](http://images.novusbio.com/fullsize/antibody/nbp2-15429_rabbit-polyclonal-apolipoprotein-a-i-apoa1-antibody-immunocytochemistry-immunofluorescence-136202310342.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein A-I/ApoA1 Antibody [NBP2-15429] -Human colon. Apolipoprotein A1 stained by Apolipoprotein A1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp2-15429_rabbit-polyclonal-apolipoprotein-a-i-apoa1-antibody-immunohistochemistry-paraffin-1362023103857..jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein A-I/ApoA1 Antibody [NBP2-15429] - Human lung. Apolipoprotein A1 stained by Apolipoprotein A1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp2-15429_rabbit-polyclonal-apolipoprotein-a-i-apoa1-antibody-immunohistochemistry-paraffin-136202310407..jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein A-I/ApoA1 Antibody [NBP2-15429] - Apolipoprotein A-I/ApoA1 antibody detects secreted Apolipoprotein A-I/ApoA1 protein by immunohistochemical analysis.Sample: Paraffin-embedded human esophagus.Apolipoprotein A-I/ApoA1 stained by Apolipoprotein A-I/ApoA1 antibody (NBP2-15429) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15429_rabbit-polyclonal-apolipoprotein-a-i-apoa1-antibody-910202420134065.jpg)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody - BSA Free [NBP2-52979] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Apolipoprotein A-1/ApoA1 Antibody (NBP2-52979) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-52979-img0006.jpg "Immunocytochemistry/Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody - BSA Free [NBP2-52979] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Apolipoprotein A-1/ApoA1 Antibody (NBP2-52979) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - Western blot analysis using Apolipoprotein A-I/ApoA1 antibody. Harvested from mouse, separated on a 4-12 gradient gel by SDS-PAGE, transferred to PVDF membrane and blocked in 3% BSA/TBST. Probed with 1:8000 anti-apoA1 in 1%BSA/TBST, and detect edwith an anti-rabbit HRP secondary antibody using chemiluminescence. WB dilution 1: 8000, block before incubation overnight. Image from verified customer review.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-Western-Blot-NBP2-52979-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody - BSA Free [NBP2-52979] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Apolipoprotein A-1/ApoA1 Antibody conjugated to FITC (NBP2-52979F) at 5ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-52979-img0007.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse liver using APOA1 antibody at 1:400 dilution. The antibody generated a weak/mild cytoplasmic signal in hepatocytes with a very strong positivity near the hepatocyte membranes as well as in the inter-cellular spaces.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-Immunohistochemistry-Paraffin-NBP2-52979-img0002.jpg)
![Flow Cytometry: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - An intracellular stain was performed on HepG2 cells with Apolipoprotein A-1/ApoA1 Antibody NBP2-52979 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, .](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-Flow-Cytometry-NBP2-52979-img0004.jpg)
![Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - Total protein from HepG2 and Hek293 cells was separated on a 4-15% gradient gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-APOA1 in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-Western-Blot-NBP2-52979-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody [NBP2-52979] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-APOA1 at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Apolipoprotein-A-I-ApoA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-52979-img0001.jpg)
![Western Blot: INSIG-1 Antibody [NB110-55244] - Detection of Insig 1 in HepG2 lyaste using NB110-55244.](http://images.novusbio.com/fullsize/INSIG-1-Antibody-Western-Blot-NB110-55244-img0002.jpg "Western Blot: INSIG-1 Antibody [NB110-55244] - Detection of Insig 1 in HepG2 lyaste using NB110-55244.")
![Immunocytochemistry/Immunofluorescence: INSIG-1 Antibody [NB110-55244] - INSIG-1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/INSIG-1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-55244-img0003.jpg)
![Western Blot: ABCA1 Antibody (1276B) [NBP2-54792] - Mouse Raw264.7 and human THP-1 cells were treated with and without the LXR agonist TO901317 (1 uM) for 24 hours. Total protein was then separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-ABCA1 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Western-Blot-NBP2-54792-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: ABCA1 Antibody (1276B) [NBP2-54792] - Mouse Raw264.7 and human THP-1 cells were treated with and without the LXR agonist TO901317 (1 uM) for 24 hours. Total protein was then separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-ABCA1 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: ABCA1 Antibody (1276B) [NBP2-54792] - Analysis of a FFPE tissue section of mouse liver using ABCA1 antibody (1276B) Lot ckjv02 at 1:100 dilution. The primary antibody bound to ABCA1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This ABCA1 antibody generated specific staining in the membranes of the hepatocytes and the Kupffer cells. As expected for ABCA1, the staining signal was stronger in the Kupffer cells which are the tissue macrophages in liver.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Immunohistochemistry-Paraffin-NBP2-54792-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ABCA1 Antibody (1276B) [NBP2-54792] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ABCA1 at 10 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Immunocytochemistry-Immunofluorescence-NBP2-54792-img0003.jpg)
![Flow (Intracellular): ABCA1 Antibody (1276B) [NBP2-54792] - An intracellular stain was performed on HepG2 Cells with ABCA1 (1276B) antibody NBP2-54792 (blue) and a matched isotype control MAB1050 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Flow-Intracellular-NBP2-54792-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ABCA1 Antibody (1276B) [NBP2-54792] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ABCA1 at 10 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Immunocytochemistry-Immunofluorescence-NBP2-54792-img0002.jpg)
![Immunohistochemistry-Paraffin: ABCA1 Antibody (1276B) [NBP2-54792] - Analysis of a FFPE tissue section of mouse liver using ABCA1 antibody (1276B) Lot ckjv0116091 at 1:50 dilution. The primary antibody bound to ABCA1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This ABCA1 antibody generated specific staining in the membranes of the hepatocytes and the Kupffer cells. As expected for ABCA1, the staining signal was stronger in the Kupffer cells which are the tissue macrophages in liver.](http://images.novusbio.com/fullsize/ABCA1-Antibody-1276B-Immunohistochemistry-Paraffin-NBP2-54792-img0005.jpg)
![Western Blot: ABCG8 Antibody [NB400-117] - Total protein from Mouse small intestine was separated on a 7.5% gel by SDS-PAGE and transferred to PVDF membrane. The membrane was then probed with anti-ABCG8 antibody at 2 ug/ml and detected with an anti-rabbit HRP conjugated secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/ABCG8-Antibody-Western-Blot-NB400-117-img0003.jpg "Western Blot: ABCG8 Antibody [NB400-117] - Total protein from Mouse small intestine was separated on a 7.5% gel by SDS-PAGE and transferred to PVDF membrane. The membrane was then probed with anti-ABCG8 antibody at 2 ug/ml and detected with an anti-rabbit HRP conjugated secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: ABCG8 Antibody [NB400-117] - ABCG8 antibody was tested in HEK293 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/ABCG8-Antibody-Immunocytochemistry-Immunofluorescence-NB400-117-img0002.jpg)