Proprotein Convertase 9/PCSK9 Antibody

Western Blot: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - (0.3ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - (5ug/ml) staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Immunohistochemistry-Paraffin: Proprotein Convertase 9/PCSK9 Antibody [NB300-959] - Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC, ChIP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Proprotein Convertase 9/PCSK9 Antibody Summary

• Description: Novus Biologicals Goat Proprotein Convertase 9/PCSK9 Antibody (NB300-959) is a polyclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and ChIP. Anti-Proprotein Convertase 9/PCSK9 Antibody: Cited in 12 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CRSRHLAQASQELQ corresponding to C-Terminus according to NP_777596.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PCSK9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5-6 ug/ml
  • Peptide ELISA detection limit 1:16000
  • Western Blot 0.3-1 ug/ml
• Application Notes: ICC/IF reactivity reported in (PMID: 30210081), Chip reactivity reported in (PMID: 20427574).
• Control:

  Proprotein Convertase 9/PCSK9 Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29593095).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Proprotein Convertase 9/PCSK9 Antibody

• EC 3.4.21
• EC 3.4.21.111
• FH3
• FH3neural apoptosis regulated convertase 1
• FHCL3
• HCHOLA3
• hypercholesterolemia, autosomal dominant 3
• LDLCQ1
• NARC1
• NARC-1
• NARC-1convertase subtilisin/kexin type 9 preproprotein
• NARC1EC 3.4.21.-
• Neural apoptosis-regulated convertase 1
• PC9
• PCSK9
• Proprotein Convertase 9
• proprotein convertase subtilisin/kexin type 9
• Subtilisin/kexin-like protease PC9

Background

This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. The encoded protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. This protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a third form of autosomal dominant familial hypercholesterolemia (HCHOLA3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Proprotein Convertase 9/PCSK9 Antibody (NB300-959)(12)

Publications using NB300-959

• Tanaka H, Inuzuka K, Iida Y et al. Proprotein Convertase Subtilisin/Kexin Type 9 Is Associated with Degenerating Adipocytes in Abdominal Aortic Aneurysm. J Oleo Sci 2018-09-13 [PMID: 30210081] (ICC/IF, Human)
• Lebeau P, Platko K, Al-Hashimi AA et al. Loss-of-function PCSK9 mutants evade the unfolded protein response sensor GRP78 and fail to induce endoplasmic reticulum stress when retained J Biol Chem 2018-05-11 [PMID: 29593095] (Mouse)
• Lebeau P, Al-Hashimi A, Sood S et al. Endoplasmic reticulum stress and Ca2+ depletion differentially modulate the sterol-regulatory protein PCSK9 to control lipid metabolism. J. Biol. Chem. 2016-12-01 [PMID: 27909053]
• Dube E, Ethier-Chiasson M, Lafond J. Modulation of cholesterol transport by insulin-treated gestational diabetes mellitus in human full-term placenta. Biol Reprod 2013-01-17 [PMID: 23221398]
• Brulhart-Meynet MC, Deakin SP, Sgroi A et al. Expression of the hepatic Niemann-Pick C1 like 1 protein gene is sensitive to rosuvastatin treatment of primary human hepatocytes. Pharmacogenet Genomics 2010-07-01 [PMID: 20461026] (WB)
• Diebold I, Petry A, Hess J et al. The NADPH oxidase subunit NOX4 is a new target gene of the hypoxia-inducible factor-1 Mol Biol Cell. 2010-06-15 [PMID: 20427574] (WB, Chemotaxis)
• Blesa S, Vernia S, Garcia-Garcia AB et al. A new PCSK9 gene promoter variant affects gene expression and causes autosomal dominant hypercholesterolemia. J Clin Endocrinol Metab. 2008-09-01 [PMID: 18559913] (WB)
  
  Details:
  WB: human depleted plasma, lymphocytes, and PCSK9 positive control cells (data described but not shown). Albumin and IgG were depleted from the plasma prior to measurement of PCSK9 expression. A WB signal was detected in positive control cells; however, P
• Dewpura T, Raymond A, Hamelin J et al. PCSK9 is phosphorylated by a Golgi casein kinase-like kinase ex vivo and circulates as a phosphoprotein in humans. FEBS J. 2008-07-01 [PMID: 18498363] (WB, Human)
  
  Details:
  WB: Media from HepG2 cells, media from PCSK9 overexpressing cells, human plasma (Fig 6). Media was IP'd with a different PCSK9 antibody and immunoprecipitates were detected by WB with the IMG-3786 PCSK9 antibody. Note: The specificity of PCSK9 antibody wa
• Lambert G, Ancellin N, Charlton F et al. Plasma PCSK9 concentrations correlate with LDL and total cholesterol in diabetic patients and are decreased by fenofibrate treatment. Clin Chem. 2008-06-01 [PMID: 18436719] (WB)
  
  Details:
  WB: Recombinant human PCSK9 (Fig 1B), PCSK9 was detected as a signal band slightly above the 60 kDa marker. ELISA: Human plasma (Figs 1D, 2, 3), The IMG-3786 PSCK9 pAb was used as the capture antibody at 1 ug/well of a 96-well ELISA plate. IP: Human hepta
• Benjannet S, Rhainds D, Hamelin J et al. The proprotein convertase (PC) PCSK9 is inactivated by furin and/or PC5/6A: functional consequences of natural mutations and post-translational modifications. J Biol Chem. 2006-10-13 [PMID: 16912035]
  
  Details:
  IP: HEK293 cells transfected with human PCSK9, PCSK9 + Furin, or empty vector (Fig 9A). The PCSK9 IMG-3786 antibody recognized pro-PCSK9, mature PCSK9, and its furin-cleaved form in both cells and media. Note: The specificity of the PCSK9 IMG-3786 antibod
• Sun XM, Eden ER, Tosi I et al. Evidence for effect of mutant PCSK9 on apolipoprotein B secretion as the cause of unusually severe dominant hypercholesterolaemia. Hum Mol Genet 2005-05-01 [PMID: 15772090]
• Cohen J, Pertsemlidis A, Kotowski IK et al. Low LDL cholesterol in individuals of African descent resulting from frequent nonsense mutations in PCSK9. Nat Genet 2005-02-01 [PMID: 15654334]

Review for Proprotein Convertase 9/PCSK9 Antibody (NB300-959) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Corey . WB Human 04/09/2013

Summary

• Application: Western Blot
• Species: Human

Bioinformatics

• Gene Symbol: PCSK9
• Entrez:
  • 255738(Human)
• Uniprot:
  • Q8NBP7()