Apolipoprotein A-I/ApoA1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody [NBP2-15429] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Apolipoprotein A1 stained by Apolipoprotein A1 antibody diluted at 1:500.Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: Apolipoprotein A-I/ApoA1 Antibody [NBP2-15429] - Human ovarian cancer. Apolipoprotein A1 stained by Apolipoprotein A1 antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NBP2-15429] - Human plasma (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Apolipoprotein A-I/ApoA1 Antibody [NBP2-15429] -HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Apolipoprotein A1 stained by Apolipoprotein A1 antibody diluted at 1:500.Blue: Fluoroshield with DAPI. Scale bar= 10um.

Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NBP2-15429] - Mouse plasma and rat plasma (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Apolipoprotein A-I/ApoA1 Antibody [NBP2-15429] -Human colon. Apolipoprotein A1 stained by Apolipoprotein A1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Apolipoprotein A-I/ApoA1 Antibody [NBP2-15429] - Human lung. Apolipoprotein A1 stained by Apolipoprotein A1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Apolipoprotein A-I/ApoA1 Antibody [NBP2-15429] - Apolipoprotein A-I/ApoA1 antibody detects secreted Apolipoprotein A-I/ApoA1 protein by immunohistochemical analysis.Sample: Paraffin-embedded human esophagus.Apolipoprotein A-I/ApoA1 stained by Apolipoprotein A-I/ApoA1 antibody (NBP2-15429) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt, SyHa
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Apolipoprotein A-I/ApoA1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Apolipoprotein A-I/ApoA1 Antibody - BSA Free (NBP2-15429) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Apolipoprotein A-I/ApoA1 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human Apolipoprotein A-I/ApoA1 Recombinant protein.
• Localization: Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APOA1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:20000
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Pig (80%). Use in Golden Syrian Hamster reported in scientific literature (PMID:36110841).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Apolipoprotein A-I/ApoA1 Antibody - BSA Free

• Alp-1
• APOA1
• Apo-AI
• apoA-I
• Apolipoprotein A1
• Apolipoprotein AI
• Apolipoprotein A-I
• Brp-14
• Ltw-1
• Lvtw-1
• MGC117399
• Sep-1
• Sep-2

Background

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apolipoprotein A-I/ApoA1 Antibody (NBP2-15429)(5)

Publications using NBP2-15429

• Ansari A, Yadav PK, Zhou L et al. MicroRNA-541-3p alters lipoproteins to reduce atherosclerosis by degrading Znf101 and Casz1 transcription factors bioRxiv : the preprint server for biology 2023-11-03 [PMID: 37961312]
• Shi H, Guo J, Yu Q et al. CRISPR/Cas9 Based Blockade of IL-10 Signaling Impairs Lipid and Tissue Homeostasis to Accelerate Atherosclerosis SSRN Electronic Journal 2022-07-01 [PMID: 36110841] (WB, Golden Syrian Hamster)
• Zhong J, Yang HC, Yermalitsky V et al. Kidney injury-mediated disruption of intestinal lymphatics involves dicarbonyl-modified lipoproteins Kidney international 2021-06-05 [PMID: 34102217]
• Tao H, Huang J, Yancey PG et al. Scavenging of reactive dicarbonyls with 2-hydroxybenzylamine reduces atherosclerosis in hypercholesterolemic Ldlr-/-mice Nat Commun 2020-08-16 [PMID: 32796843]
• Clark AJ, Jabs K, Hunley TE et al. Urinary apolipoprotein AI in children with kidney disease Pediatr. Nephrol. 2019-06-23 [PMID: 31230128]

Bioinformatics

• Gene Symbol: APOA1
• Uniprot:
  • P02647()