| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56152SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-46603SS | |||
| Azide and BSA Free | NBP2-80846 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78427SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-296SS | |||
| Azide and BSA Free | NBP2-80763 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56437SS | |||
| BSA Free | NB100-56437 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56570SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56579SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44540 | |||
| Azide and BSA Free | NBP2-34736-0.1mg | |||
![Western Blot: NOD1 Antibody [NB100-56152] - Evo-induced apoptosis of HCC cells occurred via the NOD1 pathway in vivo. (A,B) The mice treated with or without 10 mg/kg of Evo. Levels of proteins in the NOD1 pathway in tumor tissues were detected by the Western blot method. Image collected and cropped by Citeab from the following publication (Evodiamine Induces Apoptosis in SMMC-7721 and HepG2 Cells by Suppressing NOD1 Signal Pathway. Int J Mol Sci (2018)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NOD1-Antibody-Western-Blot-NB100-56152-img0003.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: NOD1 Antibody [NB100-56152] - Evo-induced apoptosis of HCC cells occurred via the NOD1 pathway in vivo. (A,B) The mice treated with or without 10 mg/kg of Evo. Levels of proteins in the NOD1 pathway in tumor tissues were detected by the Western blot method. Image collected and cropped by Citeab from the following publication (Evodiamine Induces Apoptosis in SMMC-7721 and HepG2 Cells by Suppressing NOD1 Signal Pathway. Int J Mol Sci (2018)) licensed under a CC-BY license.")
![<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Immunocytochemistry/Immunofluorescence: NOD1 Antibody [NB100-56152] - Immunocytochemistry/Immunofluorescence: NOD1 Antibody [NB100-56152] - Representative images from the immunofluorescence method (x400). HepG2 and SMMC-7721 cells were incubated with or without Evo (0, 0.5, and 1 uM) for 24 h to detect levels of NOD1 and p-P65. Values are means and standard errors of three separate experiments (* p < 0.05 and ** p < 0.01 versus control). Image collected and cropped by Citeab from the following publication (Evodiamine Induces Apoptosis in SMMC-7721 and HepG2 Cells by Suppressing NOD1 Signal Pathway. Int J Mol Sci (2018)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NOD1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56152-img0005.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: NOD1 Antibody [NB100-56152] - Evo-induced apoptosis of HCC cells occurred via the NOD1 pathway in vivo. (A,B) The mice treated with or without 10 mg/kg of Evo. Levels of proteins in the NOD1 pathway in tumor tissues were detected by the Western blot method. Image collected and cropped by Citeab from the following publication (Evodiamine Induces Apoptosis in SMMC-7721 and HepG2 Cells by Suppressing NOD1 Signal Pathway. Int J Mol Sci (2018)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NOD1-Antibody-Western-Blot-NB100-56152-img0004.jpg)
![Immunohistochemistry-Paraffin: NOD1 Antibody [NB100-56152] - Section of human sebaceous gland (A) and colon (B) stained for NOD1 expression using NB100-56152 at 1:2000. Hematoxylin counterstain. 10X magnification.](http://images.novusbio.com/fullsize/NOD1-Antibody-Immunohistochemistry-Paraffin-NB100-56152-img0002.jpg)
![Immunohistochemistry-Paraffin: NOD1 Antibody [NB100-56152] - Sections of human colon (A, A1) and pancreas (B, B1) stained for NOD1 expression using a DAB chromogen and Hematoxylin counterstain. Magnification: A (20X), A1 (40X). B (10X), B1 (40X).](http://images.novusbio.com/fullsize/NOD1-Antibody-Immunohistochemistry-Paraffin-NB100-56152-img0001.jpg)
![Western Blot: NOD1 Antibody [NB100-56152] - Evo induces apoptotic cell death of HepG2 & SMMC-7721 cells via the NOD1-mediated apoptotic pathway in vitro. HepG2 & SMMC-7721 cells were treated with 10 µg/mL IE-DAP for 2 h before exposure to 1 µM Evo. Apoptosis (A), cycle arrest (B), apoptosis-related protein levels (C) & the proteins’ levels of NOD1 pathway (D) in treated & untreated cells were measured by 5-ethynyl-2′-deoxyuridine (EdU) (×40), cellular propidium iodide (PI) fluorescence & Western blot methods. The percentage of proliferating cells (EdU+) was quantitated using ImageJ software (National Institutes of Health, Bethesda, MD, USA). Values are means & standard errors of three separate experiments (* p < 0.05 & ** p < 0.01 versus control, #p < 0.05 & ##p < 0.01 versus Evo). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30384473), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56152_rabbit-polyclonal-nod1-antibody-31020241534319.jpg)
![Western Blot: NOD1 Antibody [NB100-56152] - Evo-induced apoptosis of HCC cells occurred via the NOD1 pathway in vivo. (A,B) The mice treated with or without 10 mg/kg of Evo. Levels of proteins in the NOD1 pathway in tumor tissues were detected by the Western blot method. (C) Representative images from the immunofluorescence method. Expression of NOD1 (×400) & p-P65 (×200) in tumor tissues was analyzed by the immunofluorescence method. Values are means & standard errors of five separate experiments (* p < 0.05 & ** p < 0.01 versus control). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30384473), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56152_rabbit-polyclonal-nod1-antibody-310202415175228.jpg)
![Western Blot: NOD1 Antibody [NB100-56152] - Evo decreases expression of NOD1 resulting in suppression of nuclear factor-kappa B (NF-kappa B) & mitogen-activated protein kinase (MAPK) activation in vitro. (A) Expression of NOD1 in normal hepatocyte HL-7702, HepG2, & SMMC-7721 cells were detected by qRT-PCR & Western blot assays. (B) HepG2 & SMMC-7721 cells were incubated with 1 µM Evo for 0, 3, 6, & 12 h. The Western blot assay was performed to detect levels of proteins in the NOD1 pathway. (C) Representative images from the immunofluorescence method (×400). HepG2 & SMMC-7721 cells were incubated with or without Evo (0, 0.5, & 1 µM) for 24 h to detect levels of NOD1 & p-P65. Values are means & standard errors of three separate experiments (* p < 0.05 & ** p < 0.01 versus control). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30384473), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56152_rabbit-polyclonal-nod1-antibody-310202415293347.jpg)
![Western Blot: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - Total protein from human skin, pancreas and kidney and mouse kidney was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Melan-A in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Western-Blot-NBP2-46603-img0005.jpg "Western Blot: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - Total protein from human skin, pancreas and kidney and mouse kidney was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Melan-A in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - Analysis of FFPE tissue section of Human Skin Cancer/Melanoma using Melan-A/MART-1 antibody (A103) at 1:100 dilution. The antibody depicted very specific staining which was cytoplasmic with strong punctate appearance in peri-nuclear regions (ER and Golgi) of most of the cancerous cells and membranous-cytoplasmic pattern in some necrotic areas.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Immunohistochemistry-Paraffin-NBP2-46603-img0003.jpg)
![Flow Cytometry: Melan-A/MART-1 Antibody (A103) - BSA Free [NBP2-46603] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 Antibody (A103) NBP2-46603AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-BSA-Free-Flow-Cytometry-NBP2-46603-img0007.jpg)
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - Analysis of FFPE tissue section of Human Skin Cancer/Melanoma using Melan-A/MART-1 antibody (A103) at 1:100 dilution. The antibody depicted very specific staining which was cytoplasmic with strong punctate appearance in peri-nuclear regions (ER and Golgi) of most of the cancerous cells. The tumor stroma cells did not display any Melan-A/MART-1 expression signal.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Immunohistochemistry-Paraffin-NBP2-46603-img0002.jpg)
![Flow Cytometry: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 [A103] Antibody NBP2-46603AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Flow-Cytometry-NBP2-46603-img0004.jpg)
![Flow Cytometry: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 Antibody [A103] NBP2-46603F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Melan-A-MART-1-Antibody-A103-Flow-Cytometry-NBP2-46603-img0006.jpg)
![Flow Cytometry: TLR4 Antibody [NBP1-78427] - An intracellular stain was performed on RH-30 cells with NBP1-78427AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/TLR4-Antibody-Flow-Cytometry-NBP1-78427-img0008.jpg "Flow Cytometry: TLR4 Antibody [NBP1-78427] - An intracellular stain was performed on RH-30 cells with NBP1-78427AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Western Blot: TLR4 Antibody [NBP1-78427] - Analysis of TLR4 in Raw 264.7 cell lysate.](http://images.novusbio.com/fullsize/TLR4-Antibody-Western-Blot-NBP1-78427-img0003.jpg)
![Immunohistochemistry: TLR4 Antibody [NBP1-78427] - Analysis of TLR4 in mouse smooth muscle using DAB wtih hematoxylin counterstain.](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunohistochemistry-NBP1-78427-img0007.jpg)
![Flow Cytometry: TLR4 Antibody [NBP1-78427] - An intracellular stain was performed on RH-30 cells with TLR4 Antibody NBP1-78427AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/TLR4-Antibody-Flow-Cytometry-NBP1-78427-img0009.jpg)
![Immunohistochemistry: TLR4 Antibody [NBP1-78427] - Analysis of TLR4 in mouse spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/TLR4-Antibody-Immunohistochemistry-NBP1-78427-img0006.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [NB100-296] - HIF-1 antibody was tested in human ovarian cancer tumor xenograft using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-HA111-Immunohistochemistry-Paraffin-NB100-296-img0001.jpg "Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [NB100-296] - HIF-1 antibody was tested in human ovarian cancer tumor xenograft using DAB with hematoxylin counterstain.")
![Western Blot: HIF-1 alpha Antibody (HA111) [NB100-296] - Detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-HA111-Western-Blot-NB100-296-img0004.jpg)
![Flow Cytometry: F4/80 Antibody (BM8) [NBP1-60140] - Splenocytes isolated from a IL1R-/- mouse (IL1b receptor KO) were stained with and CD45-e450 (1:100 in FACS buffer) for 30 minutes. Cells were then washed and analyzed by flow cytometry. Left panel shows the isotype control. This image was submitted via a customer review. Image from the PE version of this antibody.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Flow-Cytometry-NBP1-60140-img0006.jpg "Flow Cytometry: F4/80 Antibody (BM8) [NBP1-60140] - Splenocytes isolated from a IL1R-/- mouse (IL1b receptor KO) were stained with and CD45-e450 (1:100 in FACS buffer) for 30 minutes. Cells were then washed and analyzed by flow cytometry. Left panel shows the isotype control. This image was submitted via a customer review. Image from the PE version of this antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Frozen: F4/80 Antibody (BM8) [NBP1-60140] - Red: F4/80, Green: CD31/PECAM-1, Blue: DAPI+ nuclei. Frozen mouse lung tissues of WT B6 and IL1R--/- (knockout) mice were blocked with 1% BSA in PBS and were double stained with PE-conjugated F4/80 and Alex647-conjugated CD31/PECAM-1 antibody (MEC 7.46) for 2 hours at the room temperature. This image was submitted via a customer review. Image from the PE version of this antibody.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Immunohistochemistry-Frozen-NBP1-60140-img0007.jpg)
![Immunohistochemistry: F4/80 Antibody (BM8) [NBP1-60140] - Expression on macrophages in mouse liver. Staining of frozen tissue section with antibody BM8.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Immunohistochemistry-NBP1-60140-img0003.jpg)
![Flow Cytometry: F4/80 Antibody (BM8) [NBP1-60140] - Analysis using the FITC conjugate of NBP1-60140. Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with BM8 FITC.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Flow-Cytometry-NBP1-60140-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of an infarcted porcine heart with anti-CD105 (MEM-229; green); cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-229-Immunofluorescence-NB110-58718-img0001.jpg "Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of an infarcted porcine heart with anti-CD105 (MEM-229; green); cell nuclei stained with DAPI (blue).")
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of CD105 on Huvec cells with anti-CD105 (MEM-229) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-229-Flow-Cytometry-NB110-58718-img0006.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Analysis of the APC conjugate of this antibody. A cell surface stain was performed on THP-1 cells with Endoglin antibody NB110-58718APC (blue) along with a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at RT.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-229-Flow-Cytometry-NB110-58718-img0002.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 antibody (MEM-229) NB110-58718PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-229-Flow-Cytometry-NB110-58718-img0003.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of CD105 on Huvec cells with anti-CD105 (MEM-229) PE.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-229-Flow-Cytometry-NB110-58718-img0004.jpg)
![Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of CD105 on Huvec cells with anti-CD105 (MEM-229) biotin.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-MEM-229-Flow-Cytometry-NB110-58718-img0005.jpg)
![Western Blot: CXCR4 Antibody - Azide Free [NBP2-24862] - Western blot shows lysates of Jurkat human T lymphocyte parental cell line and CXCR4 knockout (KO) Jurkat cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CXCR4 Polyclonal Antibody (Catalog # NB100-56437) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CXCR4 at approximately 38 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in the knockout Jurkat cell line. This experiment was conducted under reducing conditions. Image using the standard format of this product.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Western-Blot-NBP2-24862-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody - Azide Free [NBP2-24862] - Western blot shows lysates of Jurkat human T lymphocyte parental cell line and CXCR4 knockout (KO) Jurkat cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CXCR4 Polyclonal Antibody (Catalog # NB100-56437) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CXCR4 at approximately 38 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in the knockout Jurkat cell line. This experiment was conducted under reducing conditions. Image using the standard format of this product.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CXCR4 Antibody - Azide Free [NBP2-24862] - Jurkat cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-CXCR4 (NB100-56437) at a 1:25 dilution for 1 hour at room temperature and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the standard format of this product.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-24862-img0002.jpg)
![Immunohistochemistry: CXCR4 Antibody - Azide Free [NBP2-24862] - CXCR4 was detected in immersion sections of human tonsil using rabbit anti-human antibody (Catalog # NB100-56437) at 1:300 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-rabbit HRP polymer detection reagent with DAB chromogen (brown) and counterstained with hematoxylin (blue). Image using the standard format of this product.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Immunohistochemistry-NBP2-24862-img0003.jpg)
![Western Blot: CXCR4 Antibody - Azide Free [NBP2-24862] - Analysis of 15 ug of whole cell lysate from HeLa cells with anti-CXCR4 at 1 ug/ml.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Western-Blot-NBP2-24862-img0001.jpg)
![Immunohistochemistry: Influenza A H1N1 Nucleoprotein Antibody [NB100-56570] - CA/07 and NL/602 induced qualitatively similar expression of nucleoprotein in airway epithelial cells but distinct recruitment of F4/80+ cells and CD11b+ cells. Frozen lung sections from naive mice stained with anti-influenza nucleoprotein (blue), anti-F4/80 antigen (green) or anti-CD11b (red). Scale bars represent 100um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0076708) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Influenza-A-H1N1-Nucleoprotein-Antibody-Immunohistochemistry-NB100-56570-img0006.jpg "Immunohistochemistry: Influenza A H1N1 Nucleoprotein Antibody [NB100-56570] - CA/07 and NL/602 induced qualitatively similar expression of nucleoprotein in airway epithelial cells but distinct recruitment of F4/80+ cells and CD11b+ cells. Frozen lung sections from naive mice stained with anti-influenza nucleoprotein (blue), anti-F4/80 antigen (green) or anti-CD11b (red). Scale bars represent 100um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0076708) licensed under a CC-BY license.")
![Western Blot: Influenza A H1N1 Nucleoprotein Antibody [NB100-56570] - analysis of Influenza A in 50 ngs of recombinant protein using this antibody.](http://images.novusbio.com/fullsize/Influenza-A-H1N1-Nucleoprotein-Antibody-Western-Blot-NB100-56570-img0003.jpg)
![ELISA: Influenza A H1N1 Nucleoprotein Antibody [NB100-56570] - Influenza A standard curve. Influenza A monclonal antibody, clone 2F205 was used as the capture antibody. Influenza A polyclonal antibody (NB100-56570) was used as the detection antibody. Recombinant Influenza A Nucleoprotein was used as the protein standard. Researchers should empirically determine the optimal antibody concentrations for their ELISA assays.](http://images.novusbio.com/fullsize/Influenza-A-H1N1-Nucleoprotein-Antibody-ELISA-NB100-56570-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Influenza A H1N1 Nucleoprotein Antibody [NB100-56570] - CA/07 & NL/602 induced qualitatively similar expression of nucleoprotein in airway epithelial cells but distinct recruitment of F4/80+ cells & CD11b+ cells.Frozen lung sections collected two (B) & four (C) days post infection were stained with anti-influenza nucleoprotein (blue), anti-F4/80 antigen (green) or anti-CD11b (red). Both CA/07 & NL/602 induced expression of viral nucleoprotein prominently on bronchiole epithelial cells & modest expression on alveolar epithelial cells. F4/80+ & CD11b+ cells were detected in greater abundance upon infection with NL/602 compared to naïve lung. In contrast, CA/07 infection yielded substantially greater recruitment of CD11b+ cells while F4/80+ cells were minimally detected. Insets from CA/07 & NL/602 shown in 40X objective (D). Scale bars represent 100µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0076708), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56570_rabbit-polyclonal-influenza-a-h1n1-nucleoprotein-antibody-310202415535167.jpg)
![Immunocytochemistry/ Immunofluorescence: Influenza A H1N1 Nucleoprotein Antibody [NB100-56570] - CA/07 & NL/602 induced qualitatively similar expression of nucleoprotein in airway epithelial cells but distinct recruitment of F4/80+ cells & CD11b+ cells.Frozen lung sections collected two (B) & four (C) days post infection were stained with anti-influenza nucleoprotein (blue), anti-F4/80 antigen (green) or anti-CD11b (red). Both CA/07 & NL/602 induced expression of viral nucleoprotein prominently on bronchiole epithelial cells & modest expression on alveolar epithelial cells. F4/80+ & CD11b+ cells were detected in greater abundance upon infection with NL/602 compared to naïve lung. In contrast, CA/07 infection yielded substantially greater recruitment of CD11b+ cells while F4/80+ cells were minimally detected. Insets from CA/07 & NL/602 shown in 40X objective (D). Scale bars represent 100µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0076708), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56570_rabbit-polyclonal-influenza-a-h1n1-nucleoprotein-antibody-3102024165714.jpg)
![Immunocytochemistry/ Immunofluorescence: Influenza A H1N1 Nucleoprotein Antibody [NB100-56570] - CA/07 & NL/602 induced qualitatively similar expression of nucleoprotein in airway epithelial cells but distinct recruitment of F4/80+ cells & CD11b+ cells.Frozen lung sections collected two (B) & four (C) days post infection were stained with anti-influenza nucleoprotein (blue), anti-F4/80 antigen (green) or anti-CD11b (red). Both CA/07 & NL/602 induced expression of viral nucleoprotein prominently on bronchiole epithelial cells & modest expression on alveolar epithelial cells. F4/80+ & CD11b+ cells were detected in greater abundance upon infection with NL/602 compared to naïve lung. In contrast, CA/07 infection yielded substantially greater recruitment of CD11b+ cells while F4/80+ cells were minimally detected. Insets from CA/07 & NL/602 shown in 40X objective (D). Scale bars represent 100µm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0076708), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56570_rabbit-polyclonal-influenza-a-h1n1-nucleoprotein-antibody-3102024155455.jpg)
![Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - 100 ug of Mouse IgG (Lane 2) was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS 30 min at 20C, Antibody was used at 1:1000 in blocking buffer for Fluorescent Western blotting. Molecular weight markers are in lane 1. Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Western-Blot-NBP1-72739-img0005.jpg "Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - 100 ug of Mouse IgG (Lane 2) was run on a 4-20% gel and transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS 30 min at 20C, Antibody was used at 1:1000 in blocking buffer for Fluorescent Western blotting. Molecular weight markers are in lane 1. Image from the DyLight 488 version of this antibody.")
![Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - DyLight and ATTO dye conjugated antibodies provide high signal and low background for confocal microscopy and high resolution Stimulated Emission Depletion (STED) Microscopy. Both Dylight and Atto conjugated secondary antibodies maintained robust, intense signal during repeated laser excitation and de-excitation used during STED microscopy. Shown here are: A. (Green) Mouse anti NuP (NuP=Nuclear Pore Protein) detected with Dylight 488 Goat anti mouse B. (Red) Rabbit Anti Ezh1/2 Pab (Ezh=enhancer of zeste homology) with detection by ATTO 425 conjugated Goat anti Rabbit C. (Red and Green) Images combined. Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-72739-img0002.jpg)
![Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - Staining of paraffin embedded mouse tissue. Goat anti-Mouse DyLight 488 was used as a secondary antibody. Image from verified customer review. Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunohistochemistry-Paraffin-NBP1-72739-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - DyLight and ATTO dye conjugated antibodies provide high signal and low background for confocal microscopy (upper images) and high resolution Stimulated Emission Depletion (STED) Microscopy (lower images). Both Dylight and ATTO conjugated secondary antibodies maintained robust, intense signal during repeated laser excitation and de-excitation used during STED microscopy. Shown here are: A. (Green) Mouse anti NuP (NuP=Nuclear Pore Protein) detected with Dylight 488 Goat anti mouse B. (Red) Rabbit Anti Ezh1/2 Pab (Ezh=enhancer of zeste homology) with detection by ATTO 425 conjugated Goat anti Rabbit (Red and Green) Images combined. Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunocytochemistry-Immunofluorescence-NBP1-72739-img0001.jpg)
![Immunohistochemistry-Frozen: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - MDA-MB-231 cells were stained. Goat anti-Mouse DyLight 488 was used as a secondary antibody and DRAQ5 was used for nuclear stain. Image from verified customer review. Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Immunohistochemistry-Frozen-NBP1-72739-img0003.jpg)
![Dot Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - Dot Blot of Goat anti-Mouse IgG antibody Alkaline Phosphatase Conjugated Min X Bv Ch Gt GP Ham Hs Hu Rb, Rt, Sh Serum Proteins. Antigen: Mouse IgG. Load: 200 ng, 66.7 ng, 22.2 ng, 7.41 ng, or 2.47 ng as indicated.Primary antibody: Anti-Mouse IgG antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at 4C.Secondary antibody: N/A.Block: Blocking Buffer for Fluorescent Western Blotting for 60 min at RT. Image using the Alkaline Phosphatase format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Dot-Blot-NBP1-72739-img0006.jpg)
![Dot Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - A three-fold serial dilution of Mouse IgG starting at 200ug was spotted onto 0.45 um nitrocellulose. After blocking in 5% Blotto 1 Hour at 20C, Antibody was used at 1:1000 in blocking buffer for Fluorescent Western blotting. Image using the FITC format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-Dot-Blot-NBP1-72739-img0009.jpg)
![ELISA: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72739] - ELISA results of purified Goat anti-Mouse IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed) tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 ug of Mouse IgG red line) Human IgG (green line). The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using blocking buffer, Gt-anti-Donkey HRP conjugate 616-1302, and TMB substrate.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-Pre-adsorbed-ELISA-NBP1-72739-img0011.jpg)
![Western Blot: TLR4 Antibody [NB100-56579] - Analysis of TLR4 using this antibody on (A) 1 ug/lane recombinant mouse TLR4 protein (tested at 2 ug/mL), (B) 20 ug/lane human intestine, and (C) 20 ug/lane mouse intestine (tested at 5 ug/mL on intestine lysates).](http://images.novusbio.com/fullsize/TLR4-Antibody-Western-Blot-NB100-56579-img0002.jpg "Western Blot: TLR4 Antibody [NB100-56579] - Analysis of TLR4 using this antibody on (A) 1 ug/lane recombinant mouse TLR4 protein (tested at 2 ug/mL), (B) 20 ug/lane human intestine, and (C) 20 ug/lane mouse intestine (tested at 5 ug/mL on intestine lysates).")
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (SPM130) [NBP2-32831] - Formalin-fixed, paraffin-embedded human tonsil (20X) stained with CD68 MAb (SPM130).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-SPM130-Immunohistochemistry-Paraffin-NBP2-32831-img0003.jpg "Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (SPM130) [NBP2-32831] - Formalin-fixed, paraffin-embedded human tonsil (20X) stained with CD68 MAb (SPM130).")
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (SPM130) [NBP2-32831] - Formalin-fixed, paraffin-embedded human tonsil (10X) stained with CD68 MAb (SPM130).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-SPM130-Immunohistochemistry-Paraffin-NBP2-32831-img0004.jpg)
![Immunohistochemistry-Frozen: CD68/SR-D1 Antibody (SPM130) [NBP2-32831] - The antibody was incubated with feline spleen section at 1:100 and the slide was further stained with Alexa secondary antibody. Image was captured with epifluorescence microscope. This image was submitted via customer review.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-SPM130-Immunohistochemistry-Frozen-NBP2-32831-img0007.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (SPM130) [NBP2-32831] - Flow Cytometry: CD68/SR-D1 Antibody (SPM130) [Biotin] [NBP2-34736B] - Mouse peripheral blood cells were unstained (left) or stained (right) with CD68/SR-D1 antibody and anti-biotin alexa-fluor 488 secondary antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-SPM130-Flow-Cytometry-NBP2-32831-img0008.jpg)
![Immunohistochemistry: CD68/SR-D1 Antibody (SPM130) [NBP2-32831] - Intrapulmonary heterogeneity of SARS-CoV-2 host response. Selection of ROIs. Left) SARS-CoV-2 RNA-ISH staining was used to guide ROI selection of viral positive and viral negative regions. (Scale bar = 2 mm). Right) multi-color immunofluorescence staining for CD45/red (NBP2-34527AF647), CD68/yellow (NBP2-34736AF594), PanCK/green, and DNA/blue were used in parallel to select ROIs. (Scale bar = 2 mm). Example ROIs are shown in insets. (Scale bar = 100 um). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33298930/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-32831_mouse-monoclonal-cd68-sr-d1-antibody-spm130-17720241044628.jpg)
![Western Blot: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 expression in U-2 OS (Cat # L022V1).](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-Western-Blot-H00022943-M11-img0012.jpg "Western Blot: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 expression in U-2 OS (Cat # L022V1).")
![Immunohistochemistry-Paraffin: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of monoclonal antibody to DKK1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-Immunohistochemistry-Paraffin-H00022943-M11-img0009.jpg)
![Immunoprecipitation: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 transfected lysate using anti-DKK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with DKK1 rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-Immunoprecipitation-H00022943-M11-img0010.jpg)
![ELISA: Dkk-1 Antibody (2A5) [H00022943-M11] - Detection limit for recombinant GST tagged DKK1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Dkk-1-Antibody-2A5-ELISA-H00022943-M11-img0013.jpg)