Goat anti-Porcine IgA Heavy Chain Secondary Antibody

• Reactivity: Po
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Goat anti-Porcine IgA Heavy Chain Secondary Antibody Summary

• Immunogen: Pig IgA
• Specificity: By immunoelectrophoresis and ELISA this reacts specifically with pig IgA. This may cross react with IgA from other species.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:30000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:2000
  • Immunohistochemistry 1:200-1:2000
  • Immunohistochemistry-Paraffin 1:200-1:2000
  • Western Blot 1:1000- 1:30000
• Application Notes: ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgA Antibody (NB724)(18)

Publications using NB724

• Wiarda JE, Shircliff AL, Becker SR et Al. Conserved B cell signaling, activation, and differentiation in porcine jejunal and ileal Peyer's patches despite distinct immune landscapes Mucosal Immunol 2024-08-13 [PMID: 39147277]
• Paolo Trevisi, Clara Negrini, Federico Correa, Sara Virdis, Luca Laghi, Mele Marcello, Giuseppe Conte, Maurizio Mazzoni, Diana Luise Insight into the long-term impact of birth weight on intestinal development, microbial settlement, and the metabolism of weaned piglets Journal of Animal Science 2023-01-01 [PMID: 38064718]
• Wiarda J, Shircliff A, Stasko J et al. Conserved B cell signaling, activation, and differentiation in porcine jejunal and ileal Peyer's patches despite distinct immune landscapes bioRxiv 2023-09-15 (IHC)
• Johannes Schregel, Johannes Schulze Holthausen, Quentin L Sciascia, Solvig Görs, Zeyang Li, Armin Tuchscherer, Elke Albrecht, Jürgen Zentek, Cornelia C Metges Acute and persistent effects of oral glutamine supplementation on growth, cellular proliferation, and tight junction protein transcript abundance in jejunal tissue of low and normal birthweight pre-weaning piglets. PloS one 2024-01-04 [PMID: 38165864]
• Moniruzzaman M, Kim D, Kim H et al. Evaluation of dietary curcumin nanospheres as phytobiotics on growth performance, serum biochemistry, nutritional composition, meat quality, gastrointestinal health, and fecal condition of finishing pigs Frontiers in veterinary science 2023-03-08 [PMID: 36968462] (IHC)
• J Schulze Ho, J Schregel, QL Sciascia, Z Li, A Tuchschere, W Vahjen, CC Metges, J Zentek Effects of Oral Glutamine Supplementation, Birthweight and Age on Colonic Morphology and Microbiome Development in Male Suckling Piglets Microorganisms, 2022-09-25;10(10):. 2022-09-25 [PMID: 36296176]
• Schregel J, Schulze Holthausen J, Sciascia QL et al. Effects of oral glutamine supplementation on jejunal morphology, development, and amino acid profiles in male low birth weight suckling piglets PloS one 2022-04-27 [PMID: 35476806]
• Luise D, Bertocchi M, Motta V et al. Bacillus sp. probiotic supplementation diminish the Escherichia coli F4ac infection in susceptible weaned pigs by influencing the intestinal immune response, intestinal microbiota and blood metabolomics J Anim Sci Biotechnol 2019-09-19 [PMID: 31528339] (IHC-P)
• Julian Trachsel, Cassidy Briggs, Nicholas K. Gabler, Heather K. Allen, Crystal L. Loving Dietary Resistant Potato Starch Alters Intestinal Microbial Communities and Their Metabolites, and Markers of Immune Regulation and Barrier Function in Swine Frontiers in Immunology 2019-06-19 [PMID: 31275319]
• Zhou Y, Chen C, Chen Y et al. Effect of route of inoculation on innate and adaptive immune responses to porcine epidemic diarrhea virus infection in suckling pigs. Sci. Adv. 2018-11-01 [PMID: 30593385] (ELISA)
• Galiazzo G, Giancola F, Stanzani A et al. Localization of cannabinoid receptors CB1, CB2, GPR55, and PPARalpha in the canine gastrointestinal tract. Histochem Cell Biol 2018-06-07 [PMID: 29882158]
• Vincent AL, Ma W, Lager KM et al. Live attenuated influenza vaccine provides superior protection from heterologous infection in pigs with maternal antibodies without inducing vaccine-associated enhanced respiratory disease. J Virol 2012-10-01 [PMID: 22811541]
• Razzuoli E, Faggionato E, Dotti S et al. Isolation and culture of pig tonsil lymphocytes. Vet Immunol Immunopathol 2012-08-01 [PMID: 22608094]
• Bestagno M, Sola I, Dallegno E et al. Recombinant dimeric small immunoproteins neutralize transmissible gastroenteritis virus infectivity efficiently in vitro and confer passive immunity in vivo. J Gen Virol 2007-01-01 [PMID: 17170451]
• Yuan L, Honma S, Ishida S et al. Species-specific but not genotype-specific primary and secondary isotype-specific NSP4 antibody responses in gnotobiotic calves and piglets infected with homologous host bovine (NSP4[A]) or porcine (NSP4[B]) rotavirus. Virology. [PMID: 15527837]
  
  Details:
  Citation using the HRP form of this antibody.
• Nally JE, Chantranuwat C, Wu XY et al. Alveolar septal deposition of immunoglobulin and complement parallels pulmonary hemorrhage in a guinea pig model of severe pulmonary leptospirosis Am J Pathol 2004-02-26 [PMID: 14982864] (IF/IHC)
• Buhi WC, Alvarez IM. Identification, characterization and localization of three proteins expressed by the porcine oviduct. Theriogenology 2003-07-01 [PMID: 12749936]
• Thacker EL, Thacker BJ, Young TF et al. Effect of vaccination on the potentiation of porcine reproductive and respiratory syndrome virus (PRRSV)-induced pneumonia by Mycoplasma hyopneumoniae. Vaccine. [PMID: 10649626]
  
  Details:
  Citation using the HRP form of this antibody.