CD34 Antibody (ICO-115)

Images

 
Flow Cytometry: CD34 Antibody (ICO-115) [NBP2-29455] - Analysis using the PE conjugate of NBP2-29455. Surface Staining of CD34 on KG-1 cells using CD34 antibody (red) and isotype control (green) at 5 ul/0.5 ug/10^6 ...read more
Flow Cytometry: CD34 Antibody (ICO-115) [NBP2-29455] - Surface flow cytometric analysis of CD34 on KG-1 cells using CD34 antibody (red) and isotype control (green) at 0.5 ug/10^6 cells.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
ICO-115
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

CD34 Antibody (ICO-115) Summary

Immunogen
Blast cells from a chronic myeloid leukemia patient were used as the immunogen for the CD34 antibody.
Localization
Cell surface
Marker
Hematopoietic Stem Cell Marker
Specificity
This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD34
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5ug/million cells
  • Immunocytochemistry/Immunofluorescence
Application Notes
Human Chromosome Location: 1q32.2
Mol. Weight of Antigen: 90 - 110kDa. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30488141).
Reviewed Applications
Read 1 Review rated 5
using
NBP2-29455 in the following applications:

Publications
Read Publications using
NBP2-29455 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for CD34 Antibody (ICO-115)

  • CD34 antigenhematopoietic progenitor cell antigen CD34
  • CD34 molecule
  • CD34
  • HPCA1

Background

CD34 a type 1 transmembrane protein, belonging to the sialomucin family, has important roles in adhesion which remain to be fully elucidated. CD34 has an intracellular cytoplasmic domain containing consensus sites for serine, theronine, tryrosine and active protein kinase C (PKC) phosphorylation thereby implicating a role for the protein in signal transduction processes. CD34 expression, as identified by antibody, is a hallmark for identifying pluripotential hematopoietic stem or progenitor cells. CD34 antibody positive populations expand and differentiate into the various lymphohematopoietic lineages. During differentiation, lineages lose CD34 expression and become CD34 antibody negative.

CD34 is also expressed on vascular endothelium, bone marrow stroma, embryonic fibroblasts, and neurons and antibody to CD34 is also useful in analyzing these populations. Positive staining has also been described in a number of malignancies including various sarcomas and fibromas, preB-ALL, peripheral nerve sheath tumors, and papillary thyroid carcinoma. If certain kinds of tumors are characteristically positive for CD34 antibody staining such as Ewing's sarcoma, a CD34 antibody negative result may potentially rule out that particular tumor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD34 Antibody (NBP2-29455)(4)

Review for CD34 Antibody (NBP2-29455) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NBP2-29455:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen CD34 NBP2-29455
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reviewed by:
Anonymous
IHC-Fr Rat 09/20/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedBrain tissue
SpeciesRat
Lot947-1p170111

Comments

CommentsTissue Processing:The brain tissue was embedded in OCT and put in a glass beaker containing isopentane. The beaker was quickly placed in liquid nitrogen and the tissue was frozen. Removed tissue into -80 ° C for storage; Remove out the tissue from -80 ° C and equilibrate in the freezing microtome for one hour.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD34 Antibody (NBP2-29455). (Showing 1 - 1 of 1 FAQs).

  1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
    • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-33076AF350
Alexa Fluor 405 NBP2-33076AF405
Alexa Fluor 488 NBP2-33076AF488
Alexa Fluor 532 NBP2-33076AF532
Alexa Fluor 594 NBP2-33076AF594
Alexa Fluor 647 NBP2-33076AF647
Alexa Fluor 700 NBP2-33076AF700
Alexa Fluor 750 NBP2-33076AF750
Allophycocyanin NBP2-33076APC
Allophycocyanin/Cy7 NBP2-33076APCCY7
Biotin NBP2-33076B
DyLight 350 NBP2-33076UV
DyLight 405 NBP2-33076V
DyLight 488 NBP2-33076G
DyLight 550 NBP2-33076R
DyLight 594 NBP2-33076DL594
DyLight 650 NBP2-33076C
DyLight 680 NBP2-33076FR
DyLight 755 NBP2-33076IR
FITC NBP2-33076F
HRP NBP2-33076H
Janelia Fluor 549 NBP2-33076JF549
Janelia Fluor 646 NBP2-33076JF646
PE NBP2-33076PE
PE/Atto594 NBP2-33076PEATT594
PE/Cy5.5 NBP2-33076PECY55
PE/Cy7 NBP2-33076PECY7
PerCP NBP2-33076PCP

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Blogs on CD34.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

CD68 (Cluster of differentiation 68, GP110, LAMP4, SCARD1)
CD68 belongs to a growing family of hematopoietic mucin-like molecules known as lysosomal/endosomal-associated membrane glycoproteins (LAMPs). Other LAMP family members included leukosialin, stem cell antigen CD34, and GlyCAM-1. CD68 encodes a 110-kD...  Read full blog post.

CD34 (Cluster of differentiation 34, hematopoietic progenitor cell antigen)
CD34 is a cell-surface glycoprotein type 1 transmembrane protein that belongs to the sialomucin family. CD34 comprises of an intracellular cytoplasmic domain with consensus sites for serine, threonine, tyrosine and active protein kinase C (PKC). Becau...  Read full blog post.

CD34 Serves as an Important Marker in Disease Research
CD34 is a membrane protein that aids cells in cell-cell adhesion. Although little is known about its function, CD34 is an important marker for hematopoietic stem cells (HSCs), muscle satellite cells, and endothelial cells. HSCs can be found in bone ma...  Read full blog post.

CD34 Serves as an Important Marker in Disease Research
CD34 is a cell surface glycoprotein that aids cells in cell-cell adhesion. It is expressed on endothelial cells where it is known to bind L-selectin and may aid in migration of T-cells. Moreover, it is expressed on hematopoietic stem cells (HSC), musc...  Read full blog post.

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Recent Reviews

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1
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Anonymous
09/20/2017
Application: IHC-Fr
Species: Rat

Bioinformatics

Gene Symbol CD34
Uniprot