TfR (Transferrin R) Antibody (DF1513)

Western Blot: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Western Blot Analysis of Jurkat, Raji, and THP-1 cell lysate using TfR (Transferrin R) Antibody (DF1513).

Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Immunofluorescence Analysis of Human Jurkat cells labeling CD71 with TfR (Transferrin R) Antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)

Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Flow Cytometric Analysis of human Jurkat cells using TfR (Transferrin R) antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Orange); cells alone (Blue); Isotype Control (Red).

Simple Western: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Simple Western lane view shows a specific band for TfR (Transferrin R) in 0.2 mg/ml of Jurkat (left) and K562 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Electropherogram image of the corresponding Simple Western lane. TfR (Transferrin R) antibody was used at 10 ug/ml dilution of Jurkat and K562 lysates(s) respectively.

• Reactivity: Hu
• Applications: WB, Simple Western, Flow, ICC/IF
• Clone: DF1513
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

TfR (Transferrin R) Antibody (DF1513) Summary

• Additional Information: Clone DF1513 was used by HLDA to establish CD designation.
• Immunogen: KG1 acute myeloid leukemia cell line
• Localization: Cell surface
• Specificity: It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa (Workshop IV). This monoclonal antibody is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TFRC
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Simple Western 10 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34602)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for TfR (Transferrin R) Antibody (DF1513)

• CD71 antigen
• CD71
• CD71TRFR
• p90
• T9
• TfR (Transferrin R)
• TfR (TransferrinR)
• TfR
• TFR1
• TFRC
• TR
• transferrin receptor (p90, CD71)
• transferrin receptor protein 1
• TRFR

Background

Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TfR (Transferrin R) Antibody (NBP2-32945)(1)

Publication using NBP2-32945

• Ng O. Iron, microbiota and colorectal cancer. Wien Med Wochenschr. 2016-08-30 [PMID: 27577249]
  
  Details:
  This citation used the Alexa Fluor 647 version of this antibody.

Review for TfR (Transferrin R) Antibody (NBP2-32945) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Canine and Feline.

reviewed by: Michael Rosser WB Canine and Feline 06/29/2017

Summary

• Application: Western Blot
• Sample Tested: Pancreas tissue,Skin tissue,Stomach tissue,Testis tissue
• Species: Canine and Feline
• Lot: 7037-1P160929

Comments

• Comments: This antibody will be used for IHC-P in both canine and feline tissue (results pending)

Bioinformatics

• Gene Symbol: TFRC
• Uniprot:
  • P02786(Human)