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TfR (Transferrin R) Antibody (DF1513)

Images

 
Western Blot: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Western Blot Analysis of Jurkat, Raji, and THP-1 cell lysate using TfR (Transferrin R) Antibody (DF1513).
Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Immunofluorescence Analysis of Human Jurkat cells labeling CD71 with TfR (Transferrin R) Antibody (DF1513) followed by Goat ...read more
Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Flow Cytometric Analysis of human Jurkat cells using TfR (Transferrin R) antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Orange); cells alone ...read more
Simple Western: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Simple Western lane view shows a specific band for TfR (Transferrin R) in 0.2 mg/ml of Jurkat (left) and K562 (right) lysate(s). This experiment was ...read more
Simple Western: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Electropherogram image of the corresponding Simple Western lane. TfR (Transferrin R) antibody was used at 10 ug/ml dilution of Jurkat and K562 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF
Clone
DF1513
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

TfR (Transferrin R) Antibody (DF1513) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34602)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.
Additional Information
Clone DF1513 was used by HLDA to establish CD designation.
Immunogen
KG1 acute myeloid leukemia cell line
Localization
Cell surface
Specificity
It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa (Workshop IV). This monoclonal antibody is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TFRC
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Simple Western 10 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-32945 in the following applications:

Publications
Read Publication using NBP2-32945.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for TfR (Transferrin R) Antibody (DF1513)

  • CD71 antigen
  • CD71
  • CD71TRFR
  • p90
  • T9
  • TfR (Transferrin R)
  • TfR (TransferrinR)
  • TfR
  • TFR1
  • TFRC
  • TR
  • transferrin receptor (p90, CD71)
  • transferrin receptor protein 1
  • TRFR

Background

Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TfR (Transferrin R) Antibody (NBP2-32945)(1)

Review for TfR (Transferrin R) Antibody (NBP2-32945) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Canine and Feline.

Reviews using NBP2-32945:
Filter by Applications
WB
(1)
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Canine and Feline
(1)
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Images Ratings Applications Species Date Details
Western Blot TfR (Transferrin R) NBP2-32945
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4
reviewed by:
Verified Customer
WB Canine and Feline 06/29/2017
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Summary

ApplicationWestern Blot
Sample TestedPancreas tissue,Skin tissue,Stomach tissue,Testis tissue
SpeciesCanine and Feline
Lot7037-1P160929

Comments

CommentsThis antibody will be used for IHC-P in both canine and feline tissue (results pending)

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TfR (Transferrin R) Antibody (NBP2-32945) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Blogs on TfR (Transferrin R).

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

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Recent Reviews

4
5
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4
1
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1
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Verified Customer
06/29/2017
Application: WB
Species: Canine and Feline

Bioinformatics

Gene Symbol TFRC
Uniprot