| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-106SS | |||
| Azide and BSA Free | NBP2-80905 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-493SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB500-179SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1335SS | |||
| Azide and BSA Free | NBP2-80745 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-103SS | |||
| Azide and BSA Free | NBP2-80898 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47339SS | |||
| Azide and BSA Free | NBP2-80748 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-106SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-109SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-662SS | |||
| Azide and BSA Free | NBP2-80932 | |||
![Simple Western: PCNA Antibody (PC10) [NB500-106] - Image shows a specific band for PCNA in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Simple-Western-NB500-106-img0008.jpg "Simple Western: PCNA Antibody (PC10) [NB500-106] - Image shows a specific band for PCNA in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - NIH3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-PCNA Antibody (PC10) NB500-106 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Immunocytochemistry-Immunofluorescence-NB500-106-img0015.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (PC10) [NB500-106] - Analysis of paraffin-embedded human colon carcinoma, showing nuclear localization.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Immunohistochemistry-Paraffin-NB500-106-img0007.jpg)
![Western Blot: PCNA Antibody (PC10) [NB500-106] - Analysis of human (HeLa lysate), murine (SV-T2 lysate), bovine (BAEC lysate), and porcine (PAE lysate) cell extracts.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Western-Blot-NB500-106-img0006.jpg)
![Western Blot: PCNA Antibody (PC10) [NB500-106] - Western blot of PCNA levels across different stages of the cell cycle. U-2 OS cells were synchronised at G1/S with 2 mM thymidine an released for 10 hours for G2/M or re-arrested in G2 for extended duration with 10uM RO-3306. Antibody at 1:2000. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Western-Blot-NB500-106-img0014.jpg)
![Immunohistochemistry-Frozen: PCNA Antibody (PC10) [NB500-106] - Paraformaldehyde fixed frozen section of brain from murine embryo using PCNA antibody clone PC10 (green), an IB4 antibody (red) and DAPI. Image provided by Dr. Siegenthaler via product review.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Immunohistochemistry-Frozen-NB500-106-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - PC12 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-PCNA Antibody (PC10) NB500-106 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Immunocytochemistry-Immunofluorescence-NB500-106-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - HeLa cells were fixed and permeabilized for 10 minutes using cold (-20C) MeOH. The cells were incubated with anti-PCNA [PC10] at 5 ug/mL overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Immunocytochemistry-Immunofluorescence-NB500-106-img0010.jpg)
![Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Development of the germinal zones in the tree shrew neocortex. Immunofluorescence for PCNA (yellow) and DAPI staining (blue) on 30 um-cryosections of E32-P1 tree shrew neocortex. The top margin of the image corresponds to the transition zone SVZ/intermediate zone. Scale bars, 50 um. VZ, ventricular zone; SVZ, subventricular zone; iSVZ, inner SVZ; oSVZ, outer SVZ. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fnana.2018.00029/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Immunohistochemistry-NB500-106-img0013.jpg)
![Flow Cytometry: PCNA Antibody (PC10) [NB500-106] - An intracellular stain was performed on HepG2 cells with PCNA Antibody (PC10) NB500-106G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Flow-Cytometry-NB500-106-img0012.jpg)
![Western Blot: PCNA Antibody (PC10) [NB500-106] - HeLa cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Western-Blot-NB500-106-img0005.jpg)
![Western Blot: PCNA Antibody (PC10) [NB500-106] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Western-Blot-NB500-106-img0011.jpg)
![Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Analysis of PCNA in mouse cornea. Image courtesy of product review by Bo-Yie Chen.](http://images.novusbio.com/fullsize/PCNA-Antibody-PC10-Immunohistochemistry-NB500-106-img0001.jpg)
![Chromatin Immunoprecipitation: PCNA Antibody (PC10) [NB500-106] - Events taking place during a transient telomere dysfunction. All strains were cdc13–1, incubated for 160 min at 36°C to induce telomere uncapping, followed by 90 min at 23°C to allow telomere re-capping. Nocodazole & BrdU were added to the cultures at time 0. (A) Dynamics of ssDNA loss in sub-telomeres. (B) BrdU incorporation in sub-telomeres, minus incorporation at ‘CEN’ (e.g. a centromere-proximal locus, in this case ERG26). (C–F) Dynamics of the protein association with sub-telomeres, measured for Rap1 & major DNA synthesis factors (indicated above each graph). ChIP (%) was calculated as the fraction of immunoprecipitated sub-telomeric DNA, minus the fraction precipitated at ‘CEN’ (G) Dynamics of ssDNA loss at YER188W. (H) BrdU incorporation at YER188W, minus incorporation at ‘CEN’. (I–L) The protein association with YER188W was analyzed as in C–F. (M) ssDNA at ‘CEN’. (N) BrdU incorporation at ‘CEN’. (O–R) Association of proteins with ‘CEN’. (S) Growth of serial dilution of wild-type (first row) & cdc13–1 cells with or without additional mutations (indicated on the left of each row) at temperatures indicated above each plate. The plate shown on the right was cycled three times between 4 h at 36°C (to accumulate ssDNA) & 4 h at 21°C (to re-synthesize DNA), followed by incubation at 21°C for another two days. Image collected & cropped by CiteAb from the following publication (//academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkw071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-106_mouse-monoclonal-pcna-antibody-pc10-310202416154647.jpg)
![Immunocytochemistry/ Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - Development of the germinal zones in the tree shrew neocortex. (A–D) Immunofluorescence for PCNA (yellow) & DAPI staining (blue) on 30 μm-cryosections of E32–P1 tree shrew neocortex. The top margin of the image corresponds to the transition zone SVZ/intermediate zone. Scale bars, 50 μm. VZ, ventricular zone; SVZ, subventricular zone; iSVZ, inner SVZ; oSVZ, outer SVZ. (E) Quantification of the VZ thickness of the E32–P1 tree shrew neocortex. (F) Quantification of the SVZ thickness of the E32–P1 tree shrew neocortex. (G) Quantification of the VZ & SVZ thickness of the E32–P1 tree shrew neocortex, expressed as percentage of the sum of VZ & SVZ. (H) Quantification of the iSVZ & oSVZ thickness of the E37 tree shrew neocortex, expressed as percentage of the sum of iSVZ & oSVZ. (E–H) Data represent mean ± SD & were obtained from two consecutive sections of two brains each. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29725291), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-106_mouse-monoclonal-pcna-antibody-pc10-31020241615468.jpg)
![Western Blot: PINK1 Antibody [NB100-493] - Detection of murine PINK1 using NB100-493. Lane 1: molecular weight marker. Lane 2. MES cell Mitochondria (20 ug) with a band at the observed molecular weight of 63 kDa. Lane 3. MES cytosol (20 ug). Lane 4. MES nuclear (20 ug) as negative control.Lane 5. Purified human cytochrome C (0.1 ug) as PINK1 negative control.](http://images.novusbio.com/fullsize/PINK1-Antibody-Western-Blot-NB100-493-img0003.jpg "Western Blot: PINK1 Antibody [NB100-493] - Detection of murine PINK1 using NB100-493. Lane 1: molecular weight marker. Lane 2. MES cell Mitochondria (20 ug) with a band at the observed molecular weight of 63 kDa. Lane 3. MES cytosol (20 ug). Lane 4. MES nuclear (20 ug) as negative control.Lane 5. Purified human cytochrome C (0.1 ug) as PINK1 negative control.")
![Immunocytochemistry/Immunofluorescence: TPX2 Antibody [NB500-179] - Analysis of TPX2 at the mitotic spindle microtubules and poles in a HeLa metaphase cell. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TPX2-Antibody-Immunocytochemistry-Immunofluorescence-NB500-179-img0004.jpg "Immunocytochemistry/Immunofluorescence: TPX2 Antibody [NB500-179] - Analysis of TPX2 at the mitotic spindle microtubules and poles in a HeLa metaphase cell. ICC/IF image submitted by a verified customer review.")
![Flow Cytometry: TPX2 Antibody [NB500-179] - RHAMM is not a cell surface but an intracellular cytoskeletal protein in mouse embryonic stem (ES) cells. (C) Alcohol permeabilized mouse ES cells, however, were strongly positive for both RHAMM and the intracellular positive control protein TPX2 (right panel). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0073548) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TPX2-Antibody-Flow-Cytometry-NB500-179-img0005.jpg)
![Western Blot: TPX2 Antibody [NB500-179] - Analysis of TPX2 expression in 1) HeLa, 2) Ntera2, 3) K-562 and 4) Raji whole cell lysates using NB500-179.](http://images.novusbio.com/fullsize/TPX2-Antibody-Western-Blot-NB500-179-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TPX2 Antibody [NB500-179] - Staining of HeLa cells fixed in 3.5% paraformaldehyde using NB 500-179 (1:1,000). Nuclear staining during interphase and spindle staining during mitosis.](http://images.novusbio.com/fullsize/TPX2-Antibody-Immunocytochemistry-Immunofluorescence-NB500-179-img0003.jpg)
![Simple Western: TPX2 Antibody [NB500-179] - Lane view shows a specific band for TPX2 using HeLa cell lysate and antibody at 1:250. Electropherogram image of corresponding Simple Western lane view. Image reported by internal validation.](http://images.novusbio.com/fullsize/TPX2-Antibody-Simple-Western-NB500-179-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: TPX2 Antibody [NB500-179] - Depletion of TPX2 or Aurora-A reduces cell viability of BRCA2-deficient breast cancer cells. a HCC1806-shBRCA2dox, HCC38-shBRCA2dox, SUM149-shBRCA2dox, & MB231-shBRCA2dox were grown on coverslips & treated with doxycycline (3 days) and/or irradiated (IR, 5 Gy) as indicated. Subsequently, cells were stained for RAD51 & gamma H2AX. Scale bars represent 5 μm. b Quantification of results from a. Percentages of cells with ≥5 RAD51 foci per nucleus are indicated (n ≥ 31). c Percentages of cell survival of doxycycline-treated cells vs untreated cells, transfected with indicated siRNAs. Unpaired two-tailed t tests were used to test for statistical significance (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001). d BT-549 cells were transfected with siTPX2 or control siRNA (CTR). Cells were grown on coverslips for 3 days after which they were incubated with EdU conjugated to azide-Alexa 488 (10 μM) for 15 min. Subsequently, cells were fixed & stained for 53BP1 & gamma H2AX. Amounts of 53BP1 & gamma H2AX foci per cell of at least 30 EdU-positive cells were counted. Means & standard deviations are depicted. Mann–Whitney U tests were used to analyze statistical significance (*p ≤ 0.05, ** = p ≤ 0.01, ***p ≤ 0.001, ns not significant). e BT-549 cells were transfected as in d, irradiated (IR, 5 Gy), & fixated 0.5 or 6 h after irradiation. Amounts of 53BP1 & gamma H2AX foci per cell were counted. Means & standard deviations are depicted. Mann–Whitney U tests were used to analyze statistical significance (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ns = not significant) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30177840), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-179_rabbit-polyclonal-tpx2-antibody-31020241616051.jpg)
![Immunocytochemistry/ Immunofluorescence: TPX2 Antibody [NB500-179] - TPX2 depletion preferentially affects cell viability in BRCA2-deficient cancer cells. a BT-549-shBRCA2dox cells were left untreated or were treated with doxycycline (2 or 4 days), & subsequently harvested for western blotting for BRCA2 & actin. b BT-549-shBRCA2dox cells were treated as in panel A, & mRNA expression levels of BRCA2 were analyzed relative to GAPDH using qRT-PCR. c BT-549-shBRCA2dox cells were grown on coverslips & treated with doxycycline (3 days) and/or irradiated (IR, 5 Gy) as indicated. At 3 h after irradiation, cells were fixed & analyzed for RAD51 & gamma H2AX foci formation. Scale bars represent 5 μm. d Percentages of cells with ≥5 RAD51 foci per nucleus are indicated. (n ≥ 50 per condition). e BT-549-shBRCA2dox cells were treated with doxycycline (3 days) & were subsequently transfected with indicated siRNAs. A total of 30,000 cells were plated 48 h following transfection. Viable cells were counted 5 days later. Percentages of cell survival of doxycycline-treated vs untreated cells are depicted. Error bars indicate standard deviations of two experimental replicates. Unpaired two-tailed t tests were used to test for statistical significance (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30177840), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb500-179_rabbit-polyclonal-tpx2-antibody-310202416171435.jpg)
![Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - Biological and molecular analysis of cutaneous wound healing in p73+/+ and p73-/- mice. Representative micrographs of immunohistochemistry (IHC) staining for Histone H2AX [p Ser139] in skin specimens from p73+/+ and p73-/- mice 10 days after wounding. All scale bars represent 50 um. Regions of the skin are labeled as: IFE, HF, epidermal wound edge (WE), and newly-formed epidermis of the wound (W); and the dotted line indicates the border between the WE and W. *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0218458), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-NB100-2280-img0022.jpg "Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - Biological and molecular analysis of cutaneous wound healing in p73+/+ and p73-/- mice. Representative micrographs of immunohistochemistry (IHC) staining for Histone H2AX [p Ser139] in skin specimens from p73+/+ and p73-/- mice 10 days after wounding. All scale bars represent 50 um. Regions of the skin are labeled as: IFE, HF, epidermal wound edge (WE), and newly-formed epidermis of the wound (W); and the dotted line indicates the border between the WE and W. *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0218458), licensed under a CC-BY license.")
![Simple Western: Histone H2AX [p Ser139] Antibody [NB100-2280] - Simple Western lane view shows a specific band for Histone H2AX [p Ser139] in 0.2 mg/ml of Jurkat lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Simple-Western-NB100-2280-img0008.jpg)
![Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - Telomere dysfunctional induced foci (TIF) in HBV-related multistep hepatocarcinogenesis and the correlations thereof with stathmin and elongation factor 1alpha (EF1alpha) expression. A. Representative features of colocalization of Histone H2AX [p Ser139] and telomeric DNA in defined lesions of human multistep hepatocarcinogenesis. TIF are indicated by colored arrowheads: blue, DAPI; green, gamma H2AX; red, telomeres; yellow, TIF. Image collected and cropped by CiteAb from the following publication (//translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-12-154) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-NB100-2280-img0021.jpg)
![Immunohistochemistry-Paraffin: Histone H2AX [p Ser139] Antibody [NB100-2280] - Detection of human Histone H2AX [p Ser139] antibody by immunohistochemistry. Sample: FFPE section of human seminoma. Antibodies: Affinity purified rabbit Histone H2AX [p Ser139] antibody used at a dilution of 1:500. Detection: DAB.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-Paraffin-NB100-2280-img0023.jpg)
![Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - Detection of Mouse Histone H2AX [p Ser139] by Immunohistochemistry. Sample: FFPE section of mouse colon carcinoma CT26. Antibodies: Affinity purified rabbit Histone H2AX [p Ser139] antibody. Detection: DAB.](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Immunohistochemistry-NB100-2280-img0018.jpg)
![Simple Western: Histone H2AX [p Ser139] Antibody [NB100-2280] - Electropherogram image(s) of corresponding Simple Western lane view. Histone H2AX [p Ser139] antibody was used at 1:20 dilution on Jurkat lysate(s).](http://images.novusbio.com/fullsize/gamma-H2AX-[p-Ser139]-Antibody-Simple-Western-NB100-2280-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody [NB100-2280] - Treatment with anti-thyroid drug protected RPE & photoreceptors from oxidative damage induced by NaIO3.RPE & retinal oxidative damage were evaluated by immunofluorescence labeling of p-gamma H2AX & 8-OHdG on the RPE whole mounts & retinal sections at 3 days post-NaIO3 injection. a Shown are representative images of p-gamma H2AX immunofluorescence labeling on the RPE whole mounts. b Shown are representative images of p-gamma H2AX & 8-OHdG immunofluorescence labeling on the retinal sections, & corresponding quantitative analysis for p-gamma H2AX labeling. ONL, outer nuclear layer; INL, inner nuclear layer. Data represented the mean ± SEM for 5 mice per group (*p < 0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31932580), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-310202415175243.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody [NB100-2280] - Treatment with anti-thyroid drug protected RPE & photoreceptors from oxidative damage induced by NaIO3.RPE & retinal oxidative damage were evaluated by immunofluorescence labeling of p-gamma H2AX & 8-OHdG on the RPE whole mounts & retinal sections at 3 days post-NaIO3 injection. a Shown are representative images of p-gamma H2AX immunofluorescence labeling on the RPE whole mounts. b Shown are representative images of p-gamma H2AX & 8-OHdG immunofluorescence labeling on the retinal sections, & corresponding quantitative analysis for p-gamma H2AX labeling. ONL, outer nuclear layer; INL, inner nuclear layer. Data represented the mean ± SEM for 5 mice per group (*p < 0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31932580), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-310202415171325.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody [NB100-2280] - RES rescued DXR-induced apoptosis through DNA-damage-P63-Caspase3 pathway in mouse oocytes. (A) Representative immunofluorescence images showing the expression of gamma -H2AX in mouse oocytes. Green, gamma -H2AX, Blue, DNA, Bar = 20 μm. (B) The relative immunofluorescence intensity of gamma -H2AX was measured in control, DXR-treated & RES-supplemented oocytes. Experiments were repeated at least 3 times with more than 30 oocytes examined for each group. Data were presented as means ± S.E.M of three independent experiments. **means P < 0.01, *** means P < 0.001. (C) Protein levels of gamma -H2AX, P63 & Active-Caspase3 were examined by Western blotting in control, DXR-treated & RES-supplemented oocytes. GAPDH was used as a loading control. The clean backgrounds for the active-Caspase-3, gamma -H2AX & GAPDH is due to the exposure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32352929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-3102024165725.jpg)
![Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - Hepatic activation of FOXO3 induces oxidative damage & Akt activation. a Expression of Bcl2l11 (Bim), Sesn2 & Sesn3 by qPCR in livers from 9-week-old control & FOXO3CAHep transgenic mice (n = 4). b Representative IHC staining for 8-OHdG (bar = 100 μm) & quantification of 8-OHdG-positive cells as a percentage of total hepatocyte cells in livers of patients in area of small cells (SC) & large cells (LC). c Representative IHC staining for phospho-gamma H2AX (bar = 100 μm) & quantification of phospho-gamma H2AX-positive cells as a percentage of total hepatocyte cells in livers of patients in area of small cells & large cells. d Expression of Msh2 by qPCR (upper panel), Western blot analysis for MSH2 (middle panel) & densitometric quantification (lower panel) in livers from 9-week-old control & FOXO3CAHep transgenic mice (n = 4). e Western blot analysis in livers from 9-week-old control & FOXO3CAHep mice for serine-473 phospho-Akt, with Akt & GAPDH as loading control (left panel), as well as densitometric quantification (right panel). f Representative IHC staining for phospho-Akt in livers from 9-week-old control & FOXO3CAHep mice (bar = 100 μm). g Western blot analysis in livers from 9-week-old control & FOXO3CAHep mice for Rictor & GAPDH as loading control (left panel), as well as densitometric quantification (right panel) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31488102), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-310202415541940.jpg)
![Western Blot: Histone H2AX [p Ser139] Antibody [NB100-2280] - RES rescued DXR-induced apoptosis through DNA-damage-P63-Caspase3 pathway in mouse oocytes. (A) Representative immunofluorescence images showing the expression of gamma -H2AX in mouse oocytes. Green, gamma -H2AX, Blue, DNA, Bar = 20 μm. (B) The relative immunofluorescence intensity of gamma -H2AX was measured in control, DXR-treated & RES-supplemented oocytes. Experiments were repeated at least 3 times with more than 30 oocytes examined for each group. Data were presented as means ± S.E.M of three independent experiments. **means P < 0.01, *** means P < 0.001. (C) Protein levels of gamma -H2AX, P63 & Active-Caspase3 were examined by Western blotting in control, DXR-treated & RES-supplemented oocytes. GAPDH was used as a loading control. The clean backgrounds for the active-Caspase-3, gamma -H2AX & GAPDH is due to the exposure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32352929), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-31020241554196.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody [NB100-2280] - Oocyte-specific deletion of Gsk-3 beta disrupted early folliculogenesis in mice. aGsk-3 beta efficiently & specifically deleted in the oocytes in cKO mouse ovary. Immunofluorescence staining for GSK-3 beta (green) & DDX4 (red) on 1 dpp ovary. The nucleus stained by Hoechst (blue). b Control & cKO ovaries at the indicated developmental stages. Oocytes stained w/ DDX4 (green). The nucleus stained by Hoechst (blue). c Statistical analysis showed that the total number of primordial follicle decreased significantly in 7 dpp cKO ovaries (Additional file 8: Individual data values). d Apoptotic cells increased in 1 dpp cKO ovaries compared w/ the control ovaries. TUNEL signals (green) marked apoptotic cells. The nucleus stained by Hoechst (blue). e DSBs persisted in the oocytes of 1 dpp cKO ovaries. The sections stained w/ gamma -H2AX (green) & DDX4 (red). The nucleus stained by Hoechst (blue). f Ectopic RAD51 expression in the oocytes of 1 dpp cKO ovaries. The sections stained w/ RAD51 (green) & DDX4 (red). The nucleus stained by Hoechst (blue). g, h beta -catenin accumulated in the cytoplasm & translocated into nucleus of the oocytes in cKO mice. g The sections stained w/ beta -catenin (green) & DDX4 (red). The nucleus stained by Hoechst (blue). Arrowheads indicated oocytes showing nuclear beta -catenin accumulation. h The statistic analysis demonstrated that the percentage of oocytes showing beta -catenin accumulation per section increased significantly in cKO mice (Additional file 8: Individual data values). The data are presented as mean ± s.d. The asterisk (*) denotes a statistically significant difference between the control & treatment groups. *P < 0.05, **P < 0.01, & ***P < 0.001 (t test). Scale bars, 200 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30866939), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-3102024165746.jpg)
![Immunohistochemistry: Histone H2AX [p Ser139] Antibody [NB100-2280] - IDO1 inhibitor enhances the efficacy of radiotherapy in vivo. 1 × 105 of SiHa tumorsphere cells were subcutaneously injected to nude mice for tumor growth. After the tumor volume reached 50 mm3, the mice were divided into four groups of non-treated (Ctrl), INCB-024360 treated (INCB), radiotherapy (Rx), or INCB-024360 plus radiotherapy (INCB + Rx). For the INCB or INCB + Rx group, mice were injected once with 50 mg/kg INCB-024360 intraperitoneally before radiotherapy. For the Rx or INCB + Rx group, mice received 2 Gy radiation per day for total 10 Gy. Mice were sacrificed at day 30 after the last radiation treatment & the xenografted tumors were taken out for picturing (A) & weighting (B). The expression of Ki-67 or p-gamma H2AXser139 was determined by paraffin section followed by immunohistochemical staining (C). The inserted bars indicated 50 μM. The quantification results were performed by TissueFAX software (D). * p < 0.05; ** p < 0.01. The experiments were repeated two times & data from one experiment were presented. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32545442), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-31020241612641.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2AX [p Ser139] Antibody [NB100-2280] - Premature upregulation of TAp63 in GSK-3 beta -inhibited ovary. a Expression pattern of gamma -H2AX in fetal & neonatal mouse ovary in vivo. Mouse ovaries from 13.5 dpc, 15.5 dpc, 17.5 dpc, & 1 dpp immunostained for gamma -H2AX (green) & DDX4 (red). Nucleus stained by Hoechst (blue). gamma -H2AX displayed intensive expression in germ cell nucleus from 15.5 to 17.5 dpc. b Expression pattern of TAp63 in fetal & neonatal mouse ovary in vivo. Mouse ovaries from 13.5 dpc, 15.5 dpc, 17.5 dpc, 18.5 dpc, & 1 dpp immunostained for TAp63 (green) & DDX4 (red). Nucleus stained by Hoechst (blue). TAp63 protein located w/in somatic cells in fetal ovary & began to express in germ cell nucleus from 18.5 dpc afterward. c–e TAp63 expression upregulated in fetal ovary & displayed premature localization w/in oocyte nucleus following GSK-3 beta inhibition. Before examination, ovaries of 14.5 dpc cultured in vitro w/ DMSO or BIO for 3 days. c qRT-PCR analysis of TAp63 mRNA level following GSK-3 beta inhibition (normalized to beta -actin) (Additional file 8: Individual data values). d WB analysis of TAp63 protein level following GSK-3 beta inhibition. GAPDH used as internal control. e Immunofluorescence staining for TAp63 (green) & DDX4 (red). The nucleus stained by Hoechst (blue). TAp63 showed premature expression w/in oocyte nucleus following GSK-3 beta inhibition (arrowhead). f qRT-PCR results showed that relative mRNA expression level of p21, Bad, & Noxa increased significantly in GSK-3 beta -inhibiting ovaries (Additional file 8: Individual data values). data are presented as mean ± s.d. The asterisk (*) denotes a statistically significant difference between the control & treatment groups. *P < 0.05, **P < 0.01, & ***P < 0.001 (t test). Scale bars, 200 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30866939), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2280_rabbit-polyclonal-histone-h2ax-p-ser139-antibody-31020241612651.jpg)
![Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Ethanol feeding increases CHOP expression. Image from verified customer review.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-9C8-Western-Blot-NB600-1335-img0011.jpg "Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Ethanol feeding increases CHOP expression. Image from verified customer review.")
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) [NB600-1335] - FFPE tissue section of mouse brain using 1:100 dilution of GADD153/CHOP antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. The antibody generated a cytoplasmic and nuclear staining of CHOP in various cell types in the tested section.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-9C8-Immunohistochemistry-Paraffin-NB600-1335-img0010.jpg)
![Flow Cytometry: GADD153/CHOP Antibody (9C8) [NB600-1335] - An intracellular stain was performed on SK-MEL-28 cells with GADD153/CHOP Antibody (9C8) NB600-1335 (blue) and a matched isotype control MAB004 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-9C8-Flow-Cytometry-NB600-1335-img0012.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - GADD153/CHOP expression in HeLa cells treated with 2.5 ug/mL tunicamycin for 4 hours (Lane 1) and untreated (Lane 2).](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-9C8-Western-Blot-NB600-1335-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Analysis of endogenous CHOP/GADD153 from primary human fibroblasts using NB600-1335. Lane 1: Untreated cells, Lane 2: Cells treated with tunicamycin for 10 hours.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-9C8-Western-Blot-NB600-1335-img0004.jpg)
![Western Blot: GADD153/CHOP Antibody (9C8) [NB600-1335] - Analysis of CHOP in rat heart tissue lysate. Image courtesy of product review submitted by Lee Hsiao-Wei.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-9C8-Western-Blot-NB600-1335-img0005.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (9C8) [NB600-1335] - FFPE tissue section of mouse brain using 1:100 dilution of GADD153/CHOP antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. The antibody generated a cytoplasmic and nuclear staining of CHOP in various cell types in the tested section.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-9C8-Immunohistochemistry-Paraffin-NB600-1335-img0008.jpg)
![Simple Western: GADD153/CHOP Antibody (9C8) [NB600-1335] - Image shows a specific band for CHOP/GADD153 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-9C8-Simple-Western-NB600-1335-img0007.jpg)
![Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/p53-Antibody-PAb-240-Western-Blot-NB200-103-img0001.jpg "Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.")
![Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/p53-Antibody-PAb-240-Immunocytochemistry-Immunofluorescence-NB200-103-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/p53-Antibody-PAb-240-Immunohistochemistry-Paraffin-NB200-103-img0005.jpg)
![Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 Antibody [PAb 240] NB200-103 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/p53-Antibody-PAb-240-Flow-Cytometry-NB200-103-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/p53-Antibody-PAb-240-Immunocytochemistry-Immunofluorescence-NB200-103-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/p53-Antibody-PAb-240-Immunocytochemistry-Immunofluorescence-NB200-103-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - Neuro2a cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/p53-Antibody-PAb-240-Immunocytochemistry-Immunofluorescence-NB200-103-img0012.jpg)
![Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by APC-conjugated anti-mouse IgG secondary antibody F0101B.](http://images.novusbio.com/fullsize/p53-Antibody-PAb-240-Flow-Intracellular-NB200-103-img0003.jpg)
![Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/p53-Antibody-PAb-240-Flow-Cytometry-NB200-103-img0006.jpg)
![Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/p53-Antibody-PAb-240-Flow-Cytometry-NB200-103-img0007.jpg)
![Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on MCF7 cells with p53 [PAb 240] Antibody NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/p53-Antibody-PAb-240-Flow-Cytometry-NB200-103-img0008.jpg)
![Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on Neuro2a cells with p53 Antibody [PAb 240] NB200-103 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/p53-Antibody-PAb-240-Flow-Cytometry-NB200-103-img0009.jpg)
![Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-193821) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/fullsize/NDC80-Antibody-9G3-23-Western-Blot-NB100-338-img0016.jpg "Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-193821) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.")
![Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - WB analysis of NDC80 in U2OS Whole Cell Lysate. Verified customer review from 1DegreeBio.](http://images.novusbio.com/fullsize/NDC80-Antibody-9G3-23-Western-Blot-NB100-338-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - Mouse anti-Hec1 and rabbit anti-Hec1 (phospho Ser165) antibodies were used to co-stain MCF10A cells. Insets show kinetochores of misaligned chromosomes. DAPI staining shows chromatin.](http://images.novusbio.com/fullsize/NDC80-Antibody-9G3-23-Immunocytochemistry-Immunofluorescence-NB100-338-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - IF analysis of NDC80 in fixed human cell lines of RPE1, H4, and 42MGBA. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/NDC80-Antibody-9G3-23-Immunocytochemistry-Immunofluorescence-NB100-338-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - IF analysis of NDC80 in PTK1 cells.Verified customer review from 1DegreeBio.](http://images.novusbio.com/fullsize/NDC80-Antibody-9G3-23-Immunocytochemistry-Immunofluorescence-NB100-338-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nb100-338_mouse-monoclonal-ndc80-antibody-9g3-23-9102024205614.jpg)
![Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nb100-338_mouse-monoclonal-ndc80-antibody-9g3-23-91020242024619.jpg)
![Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:500.Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nb100-338_mouse-monoclonal-ndc80-antibody-9g3-23-9102024200109.jpg)
![Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nb100-338_mouse-monoclonal-ndc80-antibody-9g3-23-910202420134060.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NDC80 antibody [9G3.23] (NB100-338) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nb100-338_mouse-monoclonal-ndc80-antibody-9g3-23-western-blot-1411202518334521.jpg)
![Western Blot: GAPDH Antibody (1A10) [NBP1-47339] - Analaysis using anti-GAPDH mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate. Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Western-Blot-NBP1-47339-img0006.jpg "Western Blot: GAPDH Antibody (1A10) [NBP1-47339] - Analaysis using anti-GAPDH mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate. Theoretical molecular weight: 36 kDa.")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1A10) [NBP1-47339] - Analysis of methanol-fixed HepG2 (left) and HeLa (right) cells using anti-GAPDH mAb (green), showing cytoplasmic localization. DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Immunocytochemistry-Immunofluorescence-NBP1-47339-img0004.jpg)
![Flow Cytometry: GAPDH Antibody (1A10) [NBP1-47339] - An intracellular stain was performed on U-87 cells with GAPDH [1A10] Antibody NBP1-47339AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Flow-Cytometry-NBP1-47339-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1A10) [NBP1-47339] - GAPDH was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 2 ug/mL of mouse anti-GAPDH monoclonal (NB300-328), 2 ug/mL mL of mouse anti- GAPDH monoclonal (NBP1-47339). Cells were stained using the appropriate secondary antibody donkey anti-mouse IgG-NL557 (NL007) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Immunocytochemistry-Immunofluorescence-NBP1-47339-img0008.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody (1A10) [NBP1-47339] - Analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using anti-GAPDH mAb with DAB staining.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Immunohistochemistry-Paraffin-NBP1-47339-img0005.jpg)
![Simple Western: GAPDH Antibody (1A10) [NBP1-47339] - Lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted 36 kDa molecular weight.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Simple-Western-NBP1-47339-img0007.jpg)
![Simple Western: GAPDH Antibody [NB300-322] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted molecular weight of 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Simple-Western-NB300-322-img0005.jpg "Simple Western: GAPDH Antibody [NB300-322] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted molecular weight of 36 kDa.")
![Western Blot: GAPDH Antibody [NB300-322] - Detection of Human and Mouse GAPDH (theoretical molecular weight: 36 kDa) by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-322 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-322-img0007.jpg)
![Western Blot: GAPDH Antibody [NB300-322] - Detection of Human and Mouse GAPDH (theoretical molecular weight: 36 kDa) by Western Blot. Samples: Whole cell lysate from mouse NIH3T3 (5, 15 and 50 ug) and human HeLa (H; 50 ug) cells. Antibody: Affinity purified rabbit anti-GAPDH antibody used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-322-img0006.jpg)
![Immunohistochemistry: GAPDH Antibody [NB300-322] - Detection of human GAPDH by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-GAPDH (NB300-322). Detection: DAB](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-NB300-322-img0008.jpg)
![Western Blot: GAPDH Antibody [NB300-322] - c-Src is required for efficient translation of PRC2 component mRNAs. Immunoblot of PRC2 components in control and c-Src-deficient tumors. Bar chart shows quantification of fluorescent immunoblot data normalized to the loading control (Gapdh). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-10681-4), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-322-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-322] - GAPDH detection in HeLa cells with ICC-IF application using NB300-322, visualized with DyLight Fluor 488.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunocytochemistry-Immunofluorescence-NB300-322-img0003.jpg)
![Immunohistochemistry: GAPDH Antibody [NB300-322] - Detection of mouse GAPDH by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-GAPDH (NB300-322). Detection: DAB](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-NB300-322-img0009.jpg)
![Western Blot: GAPDH Antibody [NB300-322] - Mouse DRG stained at 1:2000 dilution. Image provided by verified customer review.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-322-img0004.jpg)
![Western Blot: GAPDH Antibody [NB300-322] - Gem expression is sufficient to increase chemokinesis and chemotaxis. Western blot analyses were performed on 70 ug of cellular extracts from MOLT4 cells transduced by Lenti-control or Lenti-GEM viral particles. Membranes were probed with anti-Gem (1:2,000) or anti-GAPDH (1:1,000) antibody. ***: significantly different, p 0.1), Fgenotype(1,30) = 30.7 (p Int J Mol Sci (2024). Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-322_rabbit-polyclonal-gapdh-antibody-12220251513483.jpg)
![Simple Western: GAPDH Antibody [NB300-322] - Astroglial expression in the plasma membrane evoked by ripple burst and fast ripple burst stimulations in wild type and S286L-TG. Panel (A) indicates expression of pannexin1 in the astroglial plasma membrane fraction. Ordinates indicate the mean ± SD (n = 6) of relative expression of pannexin1 per GAPDH. Gray and green bars indicate pannexin1 expression in astrocytes of wild type and S286L-TG after chronic fast ripple-evoked stimulation, respectively. * p 0.1), Fgenotype*age(1,20) = 5.4 (p Int J Mol Sci (2024). Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-322_rabbit-polyclonal-gapdh-antibody-12220251513482.jpg)
![Western Blot: p19ARF/CDKN2A Antibody [NB200-106] - Analysis of p19ARF/CDKN2A in (A) NIH/3T3 and (B) MEF cells.](http://images.novusbio.com/fullsize/p19ARF-CDKN2A-Antibody-Western-Blot-NB200-106-img0010.jpg "Western Blot: p19ARF/CDKN2A Antibody [NB200-106] - Analysis of p19ARF/CDKN2A in (A) NIH/3T3 and (B) MEF cells.")
![Immunocytochemistry/Immunofluorescence: p19ARF/CDKN2A Antibody [NB200-106] - Antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/p19ARF-Antibody-Immunocytochemistry-Immunofluorescence-NB200-106-img0004.jpg)
![Immunohistochemistry: p19ARF/CDKN2A Antibody [NB200-106] - Adult mouse kidney (glomerulus). Image from verified customer review.](http://images.novusbio.com/fullsize/p19ARF-Antibody-Immunocytochemistry-Immunofluorescence-NB200-106-img0006.jpg)
![Immunohistochemistry-Paraffin: p19ARF/CDKN2A Antibody [NB200-106] - Analysis of FFPE mouse spleen using p19ARF/CDKN2A antibody at 10 ug/mL on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining in the germinal centers of spleen was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/p19ARF-Antibody-Immunohistochemistry-Paraffin-NB200-106-img0005.jpg)
![Western Blot: MTH1 Antibody [NB100-109] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and MTH1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:500 of Rabbit Anti-Human MTH1 Polyclonal Antibody (Catalog # NB100-109) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for MTH1 at approximately 18 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/MTH1-Antibody-Knockout-Validated-NB100-109-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: MTH1 Antibody [NB100-109] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and MTH1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:500 of Rabbit Anti-Human MTH1 Polyclonal Antibody (Catalog # NB100-109) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for MTH1 at approximately 18 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Simple Western: MTH1 Antibody [NB100-109] - Simple Western lane view shows a specific band for MTH1 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/MTH1-Antibody-Simple-Western-NB100-109-img0007.jpg)
![Western Blot: MTH1 Antibody [NB100-109] - The cells from esophageal cancer cell lines: KYSE-450, EC109 and EC9706 (d, e), liver cancer cell lines: SMMC-7721, HepG2 and ZIP177 (f, g), gastric cancer cell lines: MGC-803, HGC-27, SGC-7901 and MKN45 (h, i), as well as the corresponding normal cell lines: Het-1A (d, e), L02 (f, g) and GES-1 (h, i) were cultured and lysed. The MTH1 protein levels were determined by Western Blot. GAPDH was used as a loading control. At least three independent experiments were performed for each group. j The cells indicated above were lysed and the total mRNA was extracted. The mRNA level of MTH1 was determined by RT-PCR. GAPDH was used as a control. At least three independent experiments were performed for each group. Image collected and cropped by Citeab from the following publication (Potent and specific MTH1 inhibitors targeting gastric cancer. Cell Death Dis (2019)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MTH1-Antibody-Western-Blot-NB100-109-img0018.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: MTH1 Antibody [NB100-109] - The antioxidant pathways are activated during EBV infection and are required for growth transformation. Freshly isolated B-lymphocytes infected with the transforming B95-8 strain of EBV were cultured for up to 2 weeks in the presence or absence of MTH1 inhibitors. Protein expression was monitored by western blots, cell proliferation and activation of the DDR were assessed by 3H-Thy incorporation and staining for I3H2AX, respectively. Representative western blots illustrating the expression of MTH1, MUTYH and OGG1 in ex vivo untreated B-cell and freshly EBV infected and SAC induced B blasts cultured for comparable times and showing similar levels of cell proliferation. Image collected and cropped by Citeab from the following publication (The Epstein-Barr virus nuclear antigen-1 upregulates the cellular antioxidant defense to enable B-cell growth transformation and immortalization. Oncogene (2020)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MTH1-Antibody-Western-Blot-NB100-109-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: MTH1 Antibody [NB100-109] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MTH1 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MTH1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-109-img0009.jpg)
![Western Blot: MTH1 Antibody [NB100-109] - EBNA1 promotes the upregulation of oxidative DNA damage repair pathways in EBV converted cell lines and EBV positive BLs. Densitometry quantification of the specific bands. The intensity of the specific band in EBV positive cells relative the EBV-negative parental is shown. Image collected and cropped by Citeab from the following publication (The Epstein-Barr virus nuclear antigen-1 upregulates the cellular antioxidant defense to enable B-cell growth transformation and immortalization. Oncogene (2020)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MTH1-Antibody-Western-Blot-NB100-109-img0012.jpg)
![Western Blot: MTH1 Antibody [NB100-109] - MTH1 antibody was tested in the labeled lanes.](http://images.novusbio.com/fullsize/MTH1-Antibody-Western-Blot-NB100-109-img0004.jpg)
![Western Blot: MTH1 Antibody [NB100-109] - Analysis of MTH1 in 1. Ntera2, 2. MCF7 cell lysate, 3. A431 cell lysate and 4. COS7 cell lysate.](http://images.novusbio.com/fullsize/MTH1-Antibody-Western-Blot-NB100-109-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MTH1 Antibody [NB100-109] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-MTH1 (NB100-109) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MTH1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-109-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MTH1 Antibody [NB100-109] - MTH1 suppression reduces the two gastric cancer cell survival. MGC-803, HGC-27 and GES-1 cells were treated by MTH1 specific siRNA 1 and #2 for 72h. Non-targeting siRNA(NT) treatment was used as control. Western Blot analysis of the protein levels of MTH1, MUTYH, OGG1, p21, ATMpS1981 and p53pS15, after the cells were treated with siRNA#2. Densitometry shows relative protein expression normalized by GAPDH. Image collected and cropped by Citeab from the following publication (Potent and specific MTH1 inhibitors targeting gastric cancer. Cell Death Dis (2019)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MTH1-Antibody-Knockdown-Validated-NB100-109-img0013.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MTH1 Antibody [NB100-109] - Simultaneous suppression of MTH1 and MYH was efficiently achieved using a two-vector system. Steps of cell line establishment are illustrated here. Top 15% of cells expressing GFP and RFP670 were sorted to improve the knockdown efficiency. Expression levels of MYH and MTH1 were analyzed after 96h of treatment with shRNA set2. Data were double normalized with GAPDH and NT-shRNA control samples and expressed as percentage. Image collected and cropped by Citeab from the following publication (hMYH and hMTH1 cooperate for survival in mismatch repair defective T-cell acute lymphoblastic leukemia. Oncogenesis (2016)) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MTH1-Antibody-Knockdown-Validated-NB100-109-img0017.jpg)
![Western Blot: MTH1 Antibody - BSA Free [NB100-109] - Compound MI-743 causes intracellular 8-oxo-dG accumulation & DNA damage.a MGC-803, HGC-27 & GES-1 cells were treated with DMSO or 5 µM MI-743 for 48 h. Intracellular 8-oxo-dG was stained with Cy3-conjugated avidin. DNA was counterstained with 4,6-diamidino-2-phenylindole (DAPI). Images were acquired at ×100 magnification by a Nikon Eclipse TE 2000-S fluorescence microscope. At least three independent experiments were performed for each group. b MGC-803, HGC-27 & GES-1 cells were treated with DMSO or 10 µM MI-743 for 48 h & run in alkaline comet assay. Pictures were originally captured at ×40 magnification. H2O2 was used as positive control. c Tail moment was calculated by CometScore software. Three individual experiments were performed for each group. d–f Western blot analysis of the protein levels of MTH1, MUTYH, OGG1, p21, ATMpS1981 & p53pS15 in MGC-803, HGC-27 & GES-1 cells, treated with increasing concentrations of MI-743 (0, 1, 2, 4, 8 & 12 µM). g, h Densitometry shows relative protein expression normalized for GAPDH. Data are representative of three independent experiments. i–k Western Blot analysis of the protein levels of MTH1, MUTYH, OGG1, p21, ATMpS1981 & p53pS15 of protein lysates, isolated from MGC-803, HGC-27 & GES-1 cells, which were treated with 12 µM MI-743 for 0, 12, 24, 36 & 48 h. l, m Densitometry shows relative protein expression normalized for GAPDH. Data are presented as means ± SD. Three individual experiments were performed for each group. *P < 0.05, **P < 0.01, ***P < 0.001 as compared with the controls Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31164636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-109_rabbit-polyclonal-mth1-antibody-310202415532071.jpg)
![Western Blot: MTH1 Antibody - BSA Free [NB100-109] - Compound MI-743 causes intracellular 8-oxo-dG accumulation & DNA damage.a MGC-803, HGC-27 & GES-1 cells were treated with DMSO or 5 µM MI-743 for 48 h. Intracellular 8-oxo-dG was stained with Cy3-conjugated avidin. DNA was counterstained with 4,6-diamidino-2-phenylindole (DAPI). Images were acquired at ×100 magnification by a Nikon Eclipse TE 2000-S fluorescence microscope. At least three independent experiments were performed for each group. b MGC-803, HGC-27 & GES-1 cells were treated with DMSO or 10 µM MI-743 for 48 h & run in alkaline comet assay. Pictures were originally captured at ×40 magnification. H2O2 was used as positive control. c Tail moment was calculated by CometScore software. Three individual experiments were performed for each group. d–f Western blot analysis of the protein levels of MTH1, MUTYH, OGG1, p21, ATMpS1981 & p53pS15 in MGC-803, HGC-27 & GES-1 cells, treated with increasing concentrations of MI-743 (0, 1, 2, 4, 8 & 12 µM). g, h Densitometry shows relative protein expression normalized for GAPDH. Data are representative of three independent experiments. i–k Western Blot analysis of the protein levels of MTH1, MUTYH, OGG1, p21, ATMpS1981 & p53pS15 of protein lysates, isolated from MGC-803, HGC-27 & GES-1 cells, which were treated with 12 µM MI-743 for 0, 12, 24, 36 & 48 h. l, m Densitometry shows relative protein expression normalized for GAPDH. Data are presented as means ± SD. Three individual experiments were performed for each group. *P < 0.05, **P < 0.01, ***P < 0.001 as compared with the controls Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31164636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-109_rabbit-polyclonal-mth1-antibody-310202415525293.jpg)
![Western Blot: MTH1 Antibody - BSA Free [NB100-109] - Characteristic expression of MTH1 in human gastric cancer tissues & ten digestive tract cancer cell lines.a The total RNA from fresh human gastric cancer tissues was isolated by RNApure Tissue&Cell Kit. The mRNA level of MTH1 in human gastric cancer (Increased, n = 21, Decreased, n = 6 & Unchanged, n = 8) & adjacent normal tissues (Con, n = 35) was determined by RT-PCR. The protein levels of MTH1 in human gastric (GC) & adjacent normal (GN) tissue sections were determined by immuohistochemistry (IHC) staining. The integral optical densities (IOD) were analyzed by Image-Pro Plus 6.0 software. b Representative IHC pictures of GC & GN. Scale bars, 50 μm. c IODs of GC & GN. n = 10 for each group. The cells from esophageal cancer cell lines: KYSE-450, EC109 & EC9706 (d, e), liver cancer cell lines: SMMC-7721, HepG2 & ZIP177 (f, g), gastric cancer cell lines: MGC-803, HGC-27, SGC-7901 & MKN45 (h, i), as well as the corresponding normal cell lines: Het-1A (d, e), L02 (f, g) & GES-1 (h, i) were cultured & lysed. The MTH1 protein levels were determined by Western Blot. GAPDH was used as a loading control. At least three independent experiments were performed for each group. j The cells indicated above were lysed & the total mRNA was extracted. The mRNA level of MTH1 was determined by RT-PCR. GAPDH was used as a control. At least three independent experiments were performed for each group. Data are presented as means ± SD. The symbol *, ** or *** stands for P < 0.05, P < 0.01 or P < 0.001 compared with the controls or normal cell groups Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31164636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-109_rabbit-polyclonal-mth1-antibody-310202415533819.jpg)
![Western Blot: MTH1 Antibody - BSA Free [NB100-109] - Compound MI-743 causes intracellular 8-oxo-dG accumulation & DNA damage.a MGC-803, HGC-27 & GES-1 cells were treated with DMSO or 5 µM MI-743 for 48 h. Intracellular 8-oxo-dG was stained with Cy3-conjugated avidin. DNA was counterstained with 4,6-diamidino-2-phenylindole (DAPI). Images were acquired at ×100 magnification by a Nikon Eclipse TE 2000-S fluorescence microscope. At least three independent experiments were performed for each group. b MGC-803, HGC-27 & GES-1 cells were treated with DMSO or 10 µM MI-743 for 48 h & run in alkaline comet assay. Pictures were originally captured at ×40 magnification. H2O2 was used as positive control. c Tail moment was calculated by CometScore software. Three individual experiments were performed for each group. d–f Western blot analysis of the protein levels of MTH1, MUTYH, OGG1, p21, ATMpS1981 & p53pS15 in MGC-803, HGC-27 & GES-1 cells, treated with increasing concentrations of MI-743 (0, 1, 2, 4, 8 & 12 µM). g, h Densitometry shows relative protein expression normalized for GAPDH. Data are representative of three independent experiments. i–k Western Blot analysis of the protein levels of MTH1, MUTYH, OGG1, p21, ATMpS1981 & p53pS15 of protein lysates, isolated from MGC-803, HGC-27 & GES-1 cells, which were treated with 12 µM MI-743 for 0, 12, 24, 36 & 48 h. l, m Densitometry shows relative protein expression normalized for GAPDH. Data are presented as means ± SD. Three individual experiments were performed for each group. *P < 0.05, **P < 0.01, ***P < 0.001 as compared with the controls Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31164636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-109_rabbit-polyclonal-mth1-antibody-310202415541950.jpg)
![Western Blot: MTH1 Antibody - BSA Free [NB100-109] - Characteristic expression of MTH1 in human gastric cancer tissues & ten digestive tract cancer cell lines.a The total RNA from fresh human gastric cancer tissues was isolated by RNApure Tissue&Cell Kit. The mRNA level of MTH1 in human gastric cancer (Increased, n = 21, Decreased, n = 6 & Unchanged, n = 8) & adjacent normal tissues (Con, n = 35) was determined by RT-PCR. The protein levels of MTH1 in human gastric (GC) & adjacent normal (GN) tissue sections were determined by immuohistochemistry (IHC) staining. The integral optical densities (IOD) were analyzed by Image-Pro Plus 6.0 software. b Representative IHC pictures of GC & GN. Scale bars, 50 μm. c IODs of GC & GN. n = 10 for each group. The cells from esophageal cancer cell lines: KYSE-450, EC109 & EC9706 (d, e), liver cancer cell lines: SMMC-7721, HepG2 & ZIP177 (f, g), gastric cancer cell lines: MGC-803, HGC-27, SGC-7901 & MKN45 (h, i), as well as the corresponding normal cell lines: Het-1A (d, e), L02 (f, g) & GES-1 (h, i) were cultured & lysed. The MTH1 protein levels were determined by Western Blot. GAPDH was used as a loading control. At least three independent experiments were performed for each group. j The cells indicated above were lysed & the total mRNA was extracted. The mRNA level of MTH1 was determined by RT-PCR. GAPDH was used as a control. At least three independent experiments were performed for each group. Data are presented as means ± SD. The symbol *, ** or *** stands for P < 0.05, P < 0.01 or P < 0.001 compared with the controls or normal cell groups Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31164636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-109_rabbit-polyclonal-mth1-antibody-310202415532076.jpg)
![Western Blot: MTH1 Antibody - BSA Free [NB100-109] - The antioxidant pathways are activated during EBV infection & are required for growth transformation. Freshly isolated B-lymphocytes infected with the transforming B95-8 strain of EBV were cultured for up to 2 weeks in the presence or absence of MTH1 inhibitors. Protein expression was monitored by western blots, cell proliferation & activation of the DDR were assessed by 3H-Thy incorporation & staining for gamma H2AX, respectively. a representative western blots illustrating the parallel increase of MTH1 & EBNA1 expression following EBV infection & mean ± SE of the intensity of the MTH1 specific band in five independent experiments. b Representative western blots illustrating the expression of MTH1, MUTYH & OGG1 in ex vivo untreated B-cell & freshly EBV infected & SAC induced B blasts cultured for comparable times & showing similar levels of cell proliferation. c Quantification of the specific bands. Relative expression is the ration between the intensity in the treated cells versus freshly harvested cells. The mean ± SE of three to four independent experiments is shown. d Inhibition of MTH1 prevents the establishment of EBV transformed lymphoblastoid cell lines. 3H-Thy incorporation was measured after culture of freshly EBV infected B-lymphocytes in the presence of the indicated amounts of MTH1 inhibitors. Depending on the condition of the cultures harvesting was done after ten to fifteen days. e Inhibition of MTH1 strongly enhances the induction of DNA damage in freshly EBV infected cells. DNA damage was detected by gamma H2AX staining. f Mean ± SE of the % gamma H2AX positive cells in three independent experiments. *P < 0.05; **P < 0.01 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31511648), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-109_rabbit-polyclonal-mth1-antibody-31020241612671.jpg)
![Western Blot: MTH1 Antibody - BSA Free [NB100-109] - EBNA1 expression is associated with upregulation of MTH1 & oxidative damage repair pathways. The expression of MTH1, OGG1, & MUTYH was investigated by western blots & qPCR in BJAB-tTAE1 cells upon addition & withdrawal of doxycycline. a Representative western blots illustrating the correlation between the reversible down- & upregulation of MTH1 & EBNA1 in BJAB-tTAE1 cells upon addition & withdrawal of doxycycline. GAPDH was used as loading control. b Densitometry quantification of MTH1 & EBNA1 expression in BJAB-tTAE1 cells. The mean intensity of the MTH1 & EBNA1 specific bands relative to GAPDH in three independent experiments is shown in the figure. c Regression analysis of the relationship between expression levels of MTH1 & EBNA1. The data from three independent experiments were used for the plot. d Representative western blots illustrating the expression of MTH1, MUTYH, & OGG1 in BJAB-tTAE1 cells cultured for two weeks in the presence or absence of doxycycline. e Fold change is the ratio between the intensity of the specific band in cells cultured without or with doxycycline. The mean ± SD of four independent experiments is shown. f qPCR analysis of the levels of MTH1, OGG1, & MUTYH transcripts in BJAB-tTAE1 cells cultured for 2 weeks in the presence or absence of doxycycline. The mean ± SE of the fold change in six independent experiments each performed in triplicate is shown Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31511648), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-109_rabbit-polyclonal-mth1-antibody-310202415535142.jpg)
![Western Blot: MTH1 Antibody - BSA Free [NB100-109] - MTH1 suppression reduces the two gastric cancer cell survival.MGC-803, HGC-27 & GES-1 cells were treated by MTH1 specific siRNA#1 & #2 for 72 h. Non-targeting siRNA(NT) treatment was used as control. a Levels of MTH1 protein then were determined by western blot. b The colony formations were recorded after the cells were treated with MTH1 siRNA#1 & #2 for 7 days. c Western Blot analysis of the protein levels of MTH1, MUTYH, OGG1, p21, ATMpS1981 & p53pS15, after the cells were treated with siRNA#2. f–h Densitometry shows relative protein expression normalized by GAPDH. d The Alkaline comets assay was performed after the cells were transfected with MTH1 siRNA#2 for 72 h. Pictures were originally captured at ×40 magnification by fluorescence microscope. Non-targeting siRNA(NT) or H2O2 treatment was used as a negative or a positive control, respectively. e Tail moment was calculated by CometScore software. Three individual experiments were performed for each group. i Relative cell viability was determined by MTT assay, after the cells were treated with MTH1 siRNA#1 & #2 with or without MI-743 combined with 100uM H2O2. Data are presented as means ± SD. Three individual experiments were performed for each group. The symbols * & #, ** & ## or ***and ### stand for P < 0.05, P < 0.01 or P < 0.001 compared with the controls Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31164636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-109_rabbit-polyclonal-mth1-antibody-31020241682184.jpg)
![Western Blot: MTH1 Antibody - BSA Free [NB100-109] - Characteristic expression of MTH1 in human gastric cancer tissues & ten digestive tract cancer cell lines.a The total RNA from fresh human gastric cancer tissues was isolated by RNApure Tissue&Cell Kit. The mRNA level of MTH1 in human gastric cancer (Increased, n = 21, Decreased, n = 6 & Unchanged, n = 8) & adjacent normal tissues (Con, n = 35) was determined by RT-PCR. The protein levels of MTH1 in human gastric (GC) & adjacent normal (GN) tissue sections were determined by immuohistochemistry (IHC) staining. The integral optical densities (IOD) were analyzed by Image-Pro Plus 6.0 software. b Representative IHC pictures of GC & GN. Scale bars, 50 μm. c IODs of GC & GN. n = 10 for each group. The cells from esophageal cancer cell lines: KYSE-450, EC109 & EC9706 (d, e), liver cancer cell lines: SMMC-7721, HepG2 & ZIP177 (f, g), gastric cancer cell lines: MGC-803, HGC-27, SGC-7901 & MKN45 (h, i), as well as the corresponding normal cell lines: Het-1A (d, e), L02 (f, g) & GES-1 (h, i) were cultured & lysed. The MTH1 protein levels were determined by Western Blot. GAPDH was used as a loading control. At least three independent experiments were performed for each group. j The cells indicated above were lysed & the total mRNA was extracted. The mRNA level of MTH1 was determined by RT-PCR. GAPDH was used as a control. At least three independent experiments were performed for each group. Data are presented as means ± SD. The symbol *, ** or *** stands for P < 0.05, P < 0.01 or P < 0.001 compared with the controls or normal cell groups Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31164636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-109_rabbit-polyclonal-mth1-antibody-31020241555213.jpg)
![Western Blot: MTH1 Antibody - BSA Free [NB100-109] - Generation of MTH1/HRASV12 BEAS2B cell lines used in this study(A). MTH1 levels are elevated in nonsmall cell lung tumors relative to adjacent normal tissue. Samples are part of a tissue array obtained from US Biomax. Tissue sections were photographed at 200X. MTH1 expression is seen as brown staining. (B). Schematic showing how cell lines for this study were generated. (C). Western blotting confirming high or low levels of HRASV12 expression in MTH1-overexpressing (pBp.MTH1) & control (pBp) BEAS2B cells. Actin is shown as a loading control. Samples were harvested & lysed approximately 19 days following transduction. Quantitation of HRAS fold-changes (pBp values set to 1) among the samples is shown below the immunoblot images. Fold changes were calculated using loading-normalized signal intensities determined from three independently run blots. (D). Western blotting confirming high & low levels of HRASV12 expression in MTH1-suppressed (shMTH1) & control (shGFP) BEAS2B cells. Actin is shown as a loading control. Samples were harvested & lysed approximately 19 days following transduction. Quantitation of HRAS fold-changes (shGFP values set to 1) among the samples is shown below the immunoblot images & was calculated using loading-normalized signal intensities determined from three independently run blots. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.3447), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-109_rabbit-polyclonal-mth1-antibody-310202415541967.jpg)
![Western Blot: MTH1 Antibody - BSA Free [NB100-109] - Compound MI-743 causes intracellular 8-oxo-dG accumulation & DNA damage.a MGC-803, HGC-27 & GES-1 cells were treated with DMSO or 5 µM MI-743 for 48 h. Intracellular 8-oxo-dG was stained with Cy3-conjugated avidin. DNA was counterstained with 4,6-diamidino-2-phenylindole (DAPI). Images were acquired at ×100 magnification by a Nikon Eclipse TE 2000-S fluorescence microscope. At least three independent experiments were performed for each group. b MGC-803, HGC-27 & GES-1 cells were treated with DMSO or 10 µM MI-743 for 48 h & run in alkaline comet assay. Pictures were originally captured at ×40 magnification. H2O2 was used as positive control. c Tail moment was calculated by CometScore software. Three individual experiments were performed for each group. d–f Western blot analysis of the protein levels of MTH1, MUTYH, OGG1, p21, ATMpS1981 & p53pS15 in MGC-803, HGC-27 & GES-1 cells, treated with increasing concentrations of MI-743 (0, 1, 2, 4, 8 & 12 µM). g, h Densitometry shows relative protein expression normalized for GAPDH. Data are representative of three independent experiments. i–k Western Blot analysis of the protein levels of MTH1, MUTYH, OGG1, p21, ATMpS1981 & p53pS15 of protein lysates, isolated from MGC-803, HGC-27 & GES-1 cells, which were treated with 12 µM MI-743 for 0, 12, 24, 36 & 48 h. l, m Densitometry shows relative protein expression normalized for GAPDH. Data are presented as means ± SD. Three individual experiments were performed for each group. *P < 0.05, **P < 0.01, ***P < 0.001 as compared with the controls Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31164636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-109_rabbit-polyclonal-mth1-antibody-31020241612661.jpg)
![Western Blot: MTH1 Antibody - BSA Free [NB100-109] - Characteristic expression of MTH1 in human gastric cancer tissues & ten digestive tract cancer cell lines.a The total RNA from fresh human gastric cancer tissues was isolated by RNApure Tissue&Cell Kit. The mRNA level of MTH1 in human gastric cancer (Increased, n = 21, Decreased, n = 6 & Unchanged, n = 8) & adjacent normal tissues (Con, n = 35) was determined by RT-PCR. The protein levels of MTH1 in human gastric (GC) & adjacent normal (GN) tissue sections were determined by immuohistochemistry (IHC) staining. The integral optical densities (IOD) were analyzed by Image-Pro Plus 6.0 software. b Representative IHC pictures of GC & GN. Scale bars, 50 μm. c IODs of GC & GN. n = 10 for each group. The cells from esophageal cancer cell lines: KYSE-450, EC109 & EC9706 (d, e), liver cancer cell lines: SMMC-7721, HepG2 & ZIP177 (f, g), gastric cancer cell lines: MGC-803, HGC-27, SGC-7901 & MKN45 (h, i), as well as the corresponding normal cell lines: Het-1A (d, e), L02 (f, g) & GES-1 (h, i) were cultured & lysed. The MTH1 protein levels were determined by Western Blot. GAPDH was used as a loading control. At least three independent experiments were performed for each group. j The cells indicated above were lysed & the total mRNA was extracted. The mRNA level of MTH1 was determined by RT-PCR. GAPDH was used as a control. At least three independent experiments were performed for each group. Data are presented as means ± SD. The symbol *, ** or *** stands for P < 0.05, P < 0.01 or P < 0.001 compared with the controls or normal cell groups Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31164636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-109_rabbit-polyclonal-mth1-antibody-310202415541912.jpg)
![Western Blot: MTH1 Antibody - BSA Free [NB100-109] - Compound MI-743 causes intracellular 8-oxo-dG accumulation & DNA damage.a MGC-803, HGC-27 & GES-1 cells were treated with DMSO or 5 µM MI-743 for 48 h. Intracellular 8-oxo-dG was stained with Cy3-conjugated avidin. DNA was counterstained with 4,6-diamidino-2-phenylindole (DAPI). Images were acquired at ×100 magnification by a Nikon Eclipse TE 2000-S fluorescence microscope. At least three independent experiments were performed for each group. b MGC-803, HGC-27 & GES-1 cells were treated with DMSO or 10 µM MI-743 for 48 h & run in alkaline comet assay. Pictures were originally captured at ×40 magnification. H2O2 was used as positive control. c Tail moment was calculated by CometScore software. Three individual experiments were performed for each group. d–f Western blot analysis of the protein levels of MTH1, MUTYH, OGG1, p21, ATMpS1981 & p53pS15 in MGC-803, HGC-27 & GES-1 cells, treated with increasing concentrations of MI-743 (0, 1, 2, 4, 8 & 12 µM). g, h Densitometry shows relative protein expression normalized for GAPDH. Data are representative of three independent experiments. i–k Western Blot analysis of the protein levels of MTH1, MUTYH, OGG1, p21, ATMpS1981 & p53pS15 of protein lysates, isolated from MGC-803, HGC-27 & GES-1 cells, which were treated with 12 µM MI-743 for 0, 12, 24, 36 & 48 h. l, m Densitometry shows relative protein expression normalized for GAPDH. Data are presented as means ± SD. Three individual experiments were performed for each group. *P < 0.05, **P < 0.01, ***P < 0.001 as compared with the controls Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31164636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-109_rabbit-polyclonal-mth1-antibody-310202415541993.jpg)
![Western Blot: MTH1 Antibody - BSA Free [NB100-109] - Screening of MTH1 inhibitors in vitro, MD simulations & binding free energy calculation of compound MI-743 & MTH1.a The structures, IC50 values & (b) percentage of MTH1 inhibition rate of the potential inhibitors MI-743, MI-401 & negative compound MI-929. c MGC-803 cells were treated with 50 µM compounds MI-743, MI-401, (S)-crizotinib or MI-929, respectively, collected & heated for 10 min from 43 to 61 °C. The cells were lysed & the protein levels of MTH1, OGG1, MUTYH or MTH2 were determined by western Blot. At least three independent experiments were performed for each group. Data are presented as means ± SD. d Predicted binding mode of compound MI-743 in MTH1 binding pocket (PDB code: 4N1U). Compound MI-743 is shown as green stick. The residues of MTH1 associated with MI-743 are shown in white. Hydrogen bonds are shown in magenta dash lines & the corresponding distances are given in Å. The RMSD value plots of the protein (MTH1) (e) & ligand (MI-743) (f) in the mutation & wild-type complex systems after 30 ns MD simulations. Binding models of compound MI-743 in MTH1 D119A (g), D120A (h), N33A (i) & W117A (j) mutations. k MM/PBSA binding free energy, estimated with the MTH1 wild-type & mutant systems obtained from the last 5 ns stable MD trajectory. l Flow diagram for the principle of detecting MTH1 activity. Since MTH1 can convert dGTP to dGMP & pyrophosphate, & the latter of which can be hydrolyzed to inorganic pyrophosphate by inorganic pyrophosphatase, the formed inorganic pyrophosphate can react with malachite green & ammonium molybdate to produce the green associated complex, the absorbance of which can be measured at 630 nm by PerkinElmer Envision microplate reader, indicating the activity of MTH1 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31164636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-109_rabbit-polyclonal-mth1-antibody-3102024155377.jpg)
![Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - HeLa cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with rRNA Antibody [Y10b] conjugated to Biotin (NB100-662B) at 5ug/ml overnight at 4C and detected with Streptavidin conjugated to DyLight 550. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-Immunocytochemistry-Immunofluorescence-NB100-662-img0015.jpg "Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - HeLa cells were fixed and permeabilized for 10 minutes with -20C MeOH. The cells were incubated with rRNA Antibody [Y10b] conjugated to Biotin (NB100-662B) at 5ug/ml overnight at 4C and detected with Streptavidin conjugated to DyLight 550. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Flow Cytometry: rRNA Antibody (Y10b) [NB100-662] - An intracellular stain was performed on HeLa cells with rRNA Antibody [Y10b] NB100-662B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-Flow-Cytometry-NB100-662-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - rRNA antibody was tested in Ntera-2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-Immunocytochemistry-Immunofluorescence-NB100-662-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - Staining of rRNA in vero cells.](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-Immunocytochemistry-Immunofluorescence-NB100-662-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: rRNA Antibody (Y10b) [NB100-662] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-rRNA (Y10b) conjugated to DyLight 488 [NB100-662G] at 5ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-Immunocytochemistry-Immunofluorescence-NB100-662-img0009.jpg)
![Immunocytochemistry: rRNA Antibody (Y10b) [NB100-662] - Immunohistochemical staining of mouse neuronal progenitor culture using rRNA antibody (green). Image from verified customer review.](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-Immunocytochemistry-NB100-662-img0005.jpg)
![Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - Analysis using Alexa Fluor (R) 488 conjugate of NB100-662. An intracellular stain was performed on HeLa cells with rRNA antibody (Y10b) NB100-662 (blue) and an isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-Flow-Intracellular-NB100-662-img0006.jpg)
![Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - Analysis of Alexa Fluor (R) 647 conjugate of NB100-662. An intracellular stain was performed on HeLa cells with rRNA (Y10b) antibody NB100-662AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1%](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-Flow-Intracellular-NB100-662-img0007.jpg)
![Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - An intracellular stain was performed on RAW 246.7 cells with rRNA Antibody (Y10b) NB100-662APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-Flow-Intracellular-NB100-662-img0008.jpg)
![Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - An intracellular stain was performed on A549 cells with NB100-662AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-Flow-Intracellular-NB100-662-img0011.jpg)
![Flow (Intracellular): rRNA Antibody (Y10b) [NB100-662] - An intracellular stain was performed on SH-SY5Y cells with rRNA (Y10b) antibody NB100-662AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-Flow-Intracellular-NB100-662-img0012.jpg)
![CyTOF-reported: rRNA Antibody (Y10b) [NB100-662] - Human bone marrow after RBC lysis, stained with anti-CD16, fixed, permeabilized with methanol, stained with anti-lactoferrin 1C6 NB120-10109 and anti-rRNA Y10b NB100-662, and gated on myeloid cells per Nat Med. 2020. 26:408-417. CyTOF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/rRNA-Antibody-Y10b-CyTOF-reported-NB100-662-img0014.jpg)
