ZNF217 Antibody

Immunohistochemistry-Paraffin: ZNF217 Antibody [NB100-41378] - 3.8ug/ml staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ZNF217 Antibody Summary

• Description: Novus Biologicals Goat ZNF217 Antibody (NB100-41378) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-ZNF217 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KNAADDSADKVNKN corresponding to internal region according to NP_006517.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ZNF217
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1:100 - 1:2000
• Application Notes: WB: Preliminary experiments gave an approx. 75 kDa band in human prostate, spleen, testis and thymus lysates after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 115 kDa band according to NP_006517.1. The 75 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human prostate shows nuclear staning in select secretory cells.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZNF217 Antibody

• DKFZp781O2147
• KIAA0335
• MGC125707
• MGC125710
• zinc finger protein 518
• zinc finger protein 518A
• ZNF518

Background

ZNF217 binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473'

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZNF217 Antibody (NB100-41378)(1)

Publication using NB100-41378

• Huang G, Krig S, Kowbel D et al. ZNF217 suppresses cell death associated with chemotherapy and telomere dysfunction. Hum Mol Genet 2005-11-01 [PMID: 16203743]

Bioinformatics

• Gene Symbol: ZNF217
• Entrez:
  • 7764(Human)
• Uniprot:
  • NP_006517.1(Human)