ZNF143 Antibody (2B4)

Images

 
Western Blot: ZNF143 Antibody (2B4) [H00007702-M01] - ZNF143 monoclonal antibody (M01), clone 2B4 Analysis of ZNF143 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: ZNF143 Antibody (2B4) [H00007702-M01] - Analysis of monoclonal antibody to ZNF143 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: ZNF143 Antibody (2B4) [H00007702-M01] - Analysis of monoclonal antibody to ZNF143 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue. Antibody concentration 3 ...read more
ELISA: ZNF143 Antibody (2B4) [H00007702-M01] - Detection limit for recombinant GST tagged ZNF143 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, ChIP
Clone
2B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

ZNF143 Antibody (2B4) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
ZNF143 (AAH20219, 1 a.a. ~ 626 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTEFPGGGMEAQHVTLCLTEAVTVADGDNLENMEGVSLQAVTLADGSTAYIQHNSKDAKLIDGQVIQLEDGSAAYVQHVPIPKSTGDSLRLEDGQAVQLEDGTTAFIHHTSKDSYDQSALQAVQLEDGTTAYIHHAVQVPQSDTILAIQADGTVAGLHTGDATIDPDTISALEQYAAKVSIDGSESVAGTGMIGENEQEKKMQIVLQGHATRVTAKSQQSGEKAFRCEYDGCGKLYTTAHHLKVHERSHTGDRPYQCEHAGCGKAFATGYGLKSHVRTHTGEKPYRCSEDNCTKSFKTSGDLQKHIRTHTGERPFKCPFEGCGRSFTTSNIRKVHVRTHTGERPYYCTEPGCGRAFASATNYKNHVRIHTGEKPYVCTVPGCDKRFTEYSSLYKHHVVHTHSKPYNCNHCGKTYKQISTLVMHKRTAHNDTEPIEEEQEAFFEPPPGQGEDVLKGSQITYVTGVEGDDVVSTQVATVTQSGLSQQVTLISQDGTQHVNISQADMQAIGNTITMVTQDGTPITVPAHDAVISSAGTHSVAMVTAEGTEGQQVAIVAQDLAAFHTASSEMGHQQHSHHLVTTETRPLTLVATSNGTQIAVQLGEQPSLEEAIRIASRIQQGETPGLDD
Specificity
ZNF143 - zinc finger protein 143 (clone pHZ-1)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ZNF143
Purity
Ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
This antibody reactivity against cell lysate and recombinant protein for WB. IT has been used for IF, IHC-P, and ELISA. ChIP usage reported in scientific literature (PMID: 25645053).
Publications
Read Publications using
H00007702-M01 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ZNF143 Antibody (2B4)

  • hStaf
  • pHZ-1
  • SBF
  • SBFzinc finger protein 143 (clone pHZ-1)
  • Selenocysteine tRNA gene transcription-activating factor
  • SPH-binding factor
  • STAF
  • STAFpHZ-1
  • transcriptional activator Staf
  • zinc finger protein 143
  • ZNF143

Background

ZNF143 is a human homolog of Xenopus transcriptional activator staf that is involved in selenocystyl tRNA transcription which binds to the SPH motif of small nuclear RNA (snRNA) gene promoters.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZNF143 Antibody (H00007702-M01)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Chemotaxis, WB.


Filter By Application
Chemotaxis
(1)
WB
(1)
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Filter By Species
Human
(2)
All Species
Showing Publications 1 - 8 of 8.
Publications using H00007702-M01 Applications Species
Shuai L, Yaqiang C, Kairong C et al. Hi-TrAC reveals division of labor of transcription factors in organizing chromatin loops. Nat Commun. 2022-11-05 [PMID: 36335136]
Witkowski MT, Lee S, Wang E et al. NUDT21 limits CD19 levels through alternative mRNA polyadenylation in B cell acute lymphoblastic leukemia Nature Immunology 2022-10-01 [PMID: 36138187]
Cobo I, Tanaka TN, Chandra Mangalhara K et al. DNA methyltransferase 3 alpha and TET methylcytosine dioxygenase 2 restrain mitochondrial DNA-mediated interferon signaling in macrophages Immunity 2022-08-09 [PMID: 35931086]
Papageorgiou DN, Karkoulia E, Amaral-Psarris A et al. Distinct and overlapping DNMT1 interactions with multiple transcription factors in erythroid cells: Evidence for co-repressor functions. Biochim Biophys Acta 2016-12-01 [PMID: 27693117]
Vinckevicius A, Parker JB, Chakravarti D. Genomic Determinants of THAP11/ZNF143/HCFC1 Complex Recruitment to Chromatin. Mol Cell Biol. 2015-12-15 [PMID: 26416877]
Bailey SD, Zhang X, Desai K et al. ZNF143 provides sequence specificity to secure chromatin interactions at gene promoters Nat Commun. 2015-02-03 [PMID: 25645053] (Chemotaxis, Human) Chemotaxis Human
Lu W, Chen Z, Zhang H et al. ZNF143 transcription factor mediates cell survival through upregulation of the GPX1 activity in the mitochondrial respiratory dysfunction Cell Death Dis 2012-11-15 [PMID: 23152058] (WB, Human) WB Human
Chen X, Fang F, Liou YC et al. Zfp143 regulates Nanog through modulation of Oct4 binding. Stem Cells;26(11):2759-67. 2008-11-01 [PMID: 18687992]

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ZNF143
Entrez
OMIM
Uniprot