ZMYM3 Antibody (PCRP-ZMYM3-2F10)

Immunocytochemistry/Immunofluorescence: ZMYM3 Antibody (PCRP-ZMYM3-2F10) [NBP3-07551] - Immunofluorescence Analysis of PFA-fixed HeLa cells using ZMYM3 Mouse Monoclonal Antibody (PCRP-ZMYM3-2F10) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).

Immunohistochemistry-Paraffin: ZMYM3 Antibody (PCRP-ZMYM3-2F10) [NBP3-07551] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with ZMYM3 Mouse Monoclonal Antibody (PCRP-ZMYM3-2F10).

Flow Cytometry: ZMYM3 Antibody (PCRP-ZMYM3-2F10) [NBP3-07551] - Flow Cytometric Analysis of PFA-fixed HeLa cells. ZMYM3 Mouse Monoclonal Antibody (PCRP-ZMYM3-2F10) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).

Flow Cytometry: ZMYM3 Antibody (PCRP-ZMYM3-2F10) [NBP3-07551] - Flow Cytometric Analysis of PFA-fixed Raji cells. ZMYM3 Mouse Monoclonal Antibody (PCRP-ZMYM3-2F10) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).

Protein Array: ZMYM3 Antibody (PCRP-ZMYM3-2F10) [NBP3-07551] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ZMYM3 Mouse Monoclonal Antibody (PCRP-ZMYM3-2F10).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, MA
• Clone: PCRP-ZMYM3-2F10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ZMYM3 Antibody (PCRP-ZMYM3-2F10) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08875)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human ZMYM3 protein (Uniprot: Q14202)
• Localization: Nucleus.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZMYM3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 152 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for ZMYM3 Antibody (PCRP-ZMYM3-2F10)

• DXS6673EZinc finger protein 261KIAA0385XFIM
• MYM
• zinc finger, MYM-type 3
• ZNF198L2
• ZNF261zinc finger MYM-type protein 3

Background

Zinc-finger protein 261(ZNF261) is a candidate gene for X-linked mental retardation. ZNF261 is a component of a multi-protein HDAC1,2 complex and associates with BHC110. ZNF261 may be a DNA binding subunit for the histone deacetylase and transcriptional repressing activity of the HDAC1,2/BHC110 complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZMYM3
• Uniprot:
  • Q14202(Human)