ZKSCAN3 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: ZKSCAN3 Antibody [NBP1-31566] - PLX4720 induces JNK2/p38 MAPK-dependent cytoplasmic translocation of ZKSCAN3. Representative confocal images of subcellular translocation of endogenous TFEB (green) and ZKSCAN3 (red) in A375 cells treated with PLX4720 (1 i1/4M, 12 h). n = 3 independent experiments. Image collected and cropped by Citeab from the following publication (Transcriptional regulation of autophagy-lysosomal function in BRAF-driven melanoma progression and chemoresistance. Nat Commun (2019) licensed under a CC-BY license.

Western Blot: ZKSCAN3 Antibody [NBP1-31566] - Western Blot: ZKSCAN3 Antibody [NBP1-31566] - Immunoblots for ZKSCAN3 in cytoplasmic and nuclear fractions of A375 cells treated with DMSO, PLX4720 (1 uM, 12 h), or Torin1 (1 uM, 3 h). Lamin B1 serves as the control for the nuclear fractions, whereas LAMP1 and Tubulin are the control of the cytoplasmic fractions. Note that PLX4720 induces cytoplasmic enrichment of ZKSCAN3, whereas Torin1 has no effect on its nucleocytoplasmic redistribution. Image collected and cropped by Citeab from the following publication (Transcriptional regulation of autophagy-lysosomal function in BRAF-driven melanoma progression and chemoresistance. Nat Commun (2019) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: ZKSCAN3 Antibody [NBP1-31566] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human lung using ZKSCAN3 antibody at 1:200 dilution. The primary antibody binding to ZKSCAN3 was detected using HRP conjugated anti-rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. Strong nuclear reactivity was observed.

Western Blot: ZKSCAN3 Antibody [NBP1-31566] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZKSCAN3 antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: ZKSCAN3 Antibody [NBP1-31566] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-ZKSCAN3 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ZKSCAN3 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human ZKSCAN3. The exact sequence is proprietary.
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZKSCAN3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZKSCAN3 Antibody - BSA Free

• dJ874C20.1
• FLJ33906
• ZF47
• Zfp47
• zfp-47
• Zinc finger and SCAN domain-containing protein 13
• Zinc finger protein 306KIAA0426
• Zinc finger protein 309ZNF306ZFP306
• Zinc finger protein 47 homolog
• zinc finger protein with KRAB and SCAN domains 3
• zinc finger protein zfp47
• zinc finger with KRAB and SCAN domains 3
• ZNF309
• ZSCAN13

Background

ZKSCAN3 may be involved in transcriptional regulation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for ZKSCAN3 Antibody (NBP1-31566)(2)

Publications using NBP1-31566

• Li, S;Song, Y;Quach, C;Guo, H;Jang, GB;Maazi, H;Zhao, S;Sands, NA;Liu, Q;In, GK;Peng, D;Yuan, W;Machida, K;Yu, M;Akbari, O;Hagiya, A;Yang, Y;Punj, V;Tang, L;Liang, C; Transcriptional regulation of autophagy-lysosomal function in BRAF-driven melanoma progression and chemoresistance Nat Commun 2019-04-12 [PMID: 30979895] (ICC/IF, Human)
• Chi Y, Xu H, Wang F et al. ZKSCAN3 promotes breast cancer cell proliferation, migration and invasion. Biochem. Biophys. Res. Commun. 2018-07-23 [PMID: 30049438] (IF/IHC, WB, Human)

Bioinformatics

• Gene Symbol: ZKSCAN3
• Entrez:
  • 80317(Human)
• Uniprot:
  • Q9BRR0(Human)