ZFP91 Antibody (8C3D5) - BSA Free

Western Blot: ZFP91 Antibody (8C3D5) [NBP2-61847] - Analysis using ZFP91 mAb against HEK293 (1) and ZFP91 (AA: 162-304)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: ZFP91 Antibody (8C3D5) [NBP2-61847] - Analysis of Hela cells using ZFP91 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry: ZFP91 Antibody (8C3D5) [NBP2-61847] - Analysis of paraffin-embedded rectum cancer tissues using ZFP91 mouse mAb with DAB staining.

Western Blot: ZFP91 Antibody (8C3D5) [NBP2-61847] - Analysis using ZFP91 mAb against human ZFP91 (AA: 162-304) recombinant protein. (Expected MW is 43 kDa)

Western Blot: ZFP91 Antibody (8C3D5) [NBP2-61847] - Analysis using ZFP91 mouse mAb against Jurkat (1), A431 (2), HepG2 (3), HEK293 (4), A549 (5), and PC-3 (6) cell lysate.

Immunocytochemistry/Immunofluorescence: ZFP91 Antibody (8C3D5) [NBP2-61847] - Analysis of Hela cells using ZFP91 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunohistochemistry: ZFP91 Antibody (8C3D5) [NBP2-61847] - Analysis of paraffin-embedded ovarian cancer tissues using ZFP91 mouse mAb with DAB staining.

ELISA: ZFP91 Antibody (8C3D5) [NBP2-61847] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

ELISA: ZFP91 Antibody (8C3D5) [NBP2-61847] - Analysis of Hela cells using ZFP91 mouse mAb (green) and negative control (red).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 8C3D5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ZFP91 Antibody (8C3D5) - BSA Free Summary

• Description: Novus Biologicals Mouse ZFP91 Antibody (8C3D5) - BSA Free (NBP2-61847) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-ZFP91 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human ZFP91 (AA: 162-304) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ZFP91
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:100- 1:500
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 44.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for ZFP91 Antibody (8C3D5) - BSA Free

• FLJ57065
• ZFP-91
• zinc finger protein 91 homolog (mouse)
• zinc finger protein homologous to Zfp91 in mouse

Background

The protein encoded by this gene is a member of the zinc finger family of proteins. The gene product contains C2H2-type domains, which are the classical zinc finger domains found in numerous nucleic acid-binding proteins. This protein functions as a regulator of the non-canonical NF-kappaB pathway in lymphotoxin-beta receptor signaling. Alternative splicing results in multiple transcript variants. A read-through transcript variant composed of ZFP91 and the downstream CNTF gene sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. A ZFP91-related pseudogene has also been identified on chromosome 2.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZFP91 Antibody (NBP2-61847)(1)

Publication using NBP2-61847

• Xing Y, Wang JY, Li MY et al. Convallatoxin inhibits IL-1 beta production by suppressing zinc finger protein 91-mediated pro-IL-1 beta ubiquitination and caspase-8 inflammasome activity British journal of pharmacology 2021-11-26 [PMID: 34825365] (ICC/IF)

Bioinformatics

• Gene Symbol: ZFP91