ZEB2 Antibody (923328) [Unconjugated]

Images

 
A549 human lung carcinoma cell line was stained with Mouse Anti-Human ZEB2/SIP1 Monoclonal Antibody (Catalog # MAB73782, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed ...read more
ZEB2/SIP1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human ZEB2/SIP1 Monoclonal Antibody (Catalog # MAB73782) at 15 µg/mL overnight at 4 °C. Tissue was stained using the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, CyTOF-ready, Flow
Clone
923328
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

ZEB2 Antibody (923328) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human ZEB2/SIP1
Asn363-Lys537
Accession # O60315
Specificity
Detects human ZEB2/SIP1 in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ZEB2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunohistochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ZEB2 Antibody (923328) [Unconjugated]

  • HSPC082
  • KIAA0569FLJ42816
  • SIP1
  • SIP1HSPC082
  • Smad-interacting protein 1
  • SMADIP1
  • SMADIP1ZFHX1BSIP-1
  • ZEB2
  • ZFHX1B
  • ZFX1B
  • zinc finger E-box binding homeobox 2
  • zinc finger E-box-binding homeobox 2
  • zinc finger homeobox 1b
  • Zinc finger homeobox protein 1b

Background

SIP1 (Smad-interacting protein 1), also called ZEB2 (Zinc finger E-box-binding homeobox 2), is a nuclear transcription factor. SIP1 contains seven C2H2-type zinc finger domains within amino acids (aa) 211-334 and 999-1076, a Smad-MHZ binding domain (aa 437-487), an atypical homeobox domain (aa 644-703), and phosphorylation, sumoylation and acetylation sites. The 1214 aa SIP1 gives a calculated molecular weight of 136 kDa, but may actually appear closer to 200 kDa due to modifications. A 1190 aa isoform lacks aa 111-134. Within aa 363-537, human SIP1 shares 98% and 97% aa sequence identity with mouse and rat SIP1, respectively. SIP1 is highly expressed in postmitotic neocortical cells and influences cell fate decisions in embryonic brain development. Point mutations causing underexpression of SIP1 are associated with Mowat-Wilson syndrome (MWIS), also known as Hirschprung disease mental retardation syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ZEB2
Uniprot