ZEB2 Antibody (923328) [Alexa Fluor® 647]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
923328
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 647

Order Details

ZEB2 Antibody (923328) [Alexa Fluor® 647] Summary

Immunogen
E. coli-derived recombinant human ZEB2/SIP1
Asn363-Lys537
Accession # O60315
Specificity
Detects human ZEB2/SIP1 in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ZEB2 Antibody (923328) [Alexa Fluor® 647]

  • HSPC082
  • KIAA0569FLJ42816
  • SIP1
  • SIP1HSPC082
  • Smad-interacting protein 1
  • SMADIP1
  • SMADIP1ZFHX1BSIP-1
  • ZEB2
  • ZFHX1B
  • ZFX1B
  • zinc finger E-box binding homeobox 2
  • zinc finger E-box-binding homeobox 2
  • zinc finger homeobox 1b
  • Zinc finger homeobox protein 1b

Background

SIP1 (Smad-interacting protein 1), also called ZEB2 (Zinc finger E-box-binding homeobox 2), is a nuclear transcription factor. SIP1 contains seven C2H2-type zinc finger domains within amino acids (aa) 211-334 and 999-1076, a Smad-MHZ binding domain (aa 437-487), an atypical homeobox domain (aa 644-703), and phosphorylation, sumoylation and acetylation sites. The 1214 aa SIP1 gives a calculated molecular weight of 136 kDa, but may actually appear closer to 200 kDa due to modifications. A 1190 aa isoform lacks aa 111-134. Within aa 363-537, human SIP1 shares 98% and 97% aa sequence identity with mouse and rat SIP1, respectively. SIP1 is highly expressed in postmitotic neocortical cells and influences cell fate decisions in embryonic brain development. Point mutations causing underexpression of SIP1 are associated with Mowat-Wilson syndrome (MWIS), also known as Hirschprung disease mental retardation syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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