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ZEB2 Antibody (2H5)

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Western Blot: ZEB2 Antibody (2H5) [H00009839-M02] - ZFHX1B monoclonal antibody (M02), clone 2H5. Analysis of ZFHX1B expression in HepG2.
Western Blot: ZEB2 Antibody (2H5) [H00009839-M02] - ZFHX1B monoclonal antibody (M02), clone 2H5. Analysis of ZFHX1B expression in HepG2.
Western Blot: ZEB2 Antibody (2H5) [H00009839-M02] - ZFHX1B monoclonal antibody (M02), clone 2H5. Analysis of ZFHX1B expression in human ovarian cancer.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
2H5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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ZEB2 Antibody (2H5) Summary

Immunogen
ZFHX1B (NP_055610.1, 1115 a.a. ~ 1214 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SITPQGYSDSEERESMPRDGESEKEHEKEGEDGYGKLGRQDGDEEFEEEEEESENKSMDTDPETIRDEEETGDHSMDDSSEDGKMETKSDHEEDNMEDGM
Specificity
ZFHX1B - zinc finger homeobox 1b (2H5)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ZEB2
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate, tissue lysate and recombinant protein for western blot. It has also been used for ELISA.
Reviewed Applications
Read 1 Review rated 4
using
H00009839-M02 in the following applications:
Publications
Read Publication using
H00009839-M02 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ZEB2 Antibody (2H5)

  • HSPC082
  • KIAA0569FLJ42816
  • SIP1
  • SIP1HSPC082
  • Smad-interacting protein 1
  • SMADIP1
  • SMADIP1ZFHX1BSIP-1
  • ZEB2
  • ZFHX1B
  • ZFX1B
  • zinc finger E-box binding homeobox 2
  • zinc finger E-box-binding homeobox 2
  • zinc finger homeobox 1b
  • Zinc finger homeobox protein 1b

Background

The SMADIP1 gene (also known as SIP1) is a member of the delta-EF1 (ZEB1; MIM 189909)/Zfh1 family of 2-handed zinc finger/homeodomain proteins. SMADIP1 interacts with receptor-mediated, activated full-length SMADs (see MIM 605568) (Verschueren et al., 1999 [PubMed 10400677]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ZEB1 Antibody
NBP1-05987
Immunoprecipitation: ZEB1 Antibody [NBP1-05987] - Detection of human ZEB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZEB1 antibody NBP1-05987 used for IP at 3 ug per reaction. ZEB1 was also immunoprecipitated by another rabbit anti-ZEB1 antibody. For blotting immunoprecipitated ZEB1, NBP1-05987 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: ZEB1 Antibody [NBP1-05987] - Immunohistochemical staining results of EMT regulators in HG-PanIN: SNAIL, SLUG, TWIST1, and ZEB1. We categorized and defined three grades: grade 0, <10% positive staining; grade 1, 10%-50% positive; grade 2, 50%< positive Image collected and cropped by CiteAb from the following publication (https://onlinelibrary.wiley.com/doi/abs/10.1002/cam4.2016) licensed under a CC-BY licence.
Western Blot: ZEB1 Antibody [NBP1-05987] - Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZEB1 antibody (NBP1-05987) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
(6 Reviews)
(79 Publications)
Species: Hu, Mu, Rt
Applications: ChIP, GS, ICC/IF, IHC, IHC-P, IP, KD, MiAr, Simple Western, Single-Cell Western, WB
E-Cadherin
Antibody [Unconjugate
AF748
E‑Cadherin was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human/MouseE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
E‑Cadherin was detected in perfusion fixed frozen sections of mouse skin using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes in keratinocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
E‑Cadherin was detected in immersion fixed mouse intestinal organoids using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL001) and counterstained with DAPI (blue). Magnification shown at 100X (upper panel) and 40X (lower panel). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(55 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
SLC22A3 Antibody
NBP1-83976
Immunohistochemistry-Paraffin: SLC22A3 Antibody [NBP1-83976] - Staining of human prostate shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: SLC22A3 Antibody [NBP1-83976] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: SLC22A3 Antibody [NBP1-83976] - Staining of human fallopian tube shows moderate membranous and cytoplasmic positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
ITK Antibody (5G6)
NBP2-37578
ELISA: ITK Antibody (5G6) [NBP2-37578] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Western Blot: ITK Antibody (5G6) [NBP2-37578] - Analysis using ITK mAb against human ITK (AA: 2-120) recombinant protein. (Expected MW is 39.7 kDa)
Western Blot: ITK Antibody (5G6) [NBP2-37578] - Analysis using ITK mouse mAb against Jurkat cell lysate.
Species: Hu
Applications: ELISA, Flow, ICC/IF, WB
Slug Antibody
(OTI1A6)
NBP2-03886
Flow Cytometry: SLUG Antibody (1A6) [NBP2-03886] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SLUG antibody, and then analyzed by flow cytometry.
Western Blot: SLUG Antibody (1A6) [NBP2-03886] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLUG (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLUG.
Immunohistochemistry-Paraffin: SLUG Antibody (1A6) [NBP2-03886] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SLUG mouse monoclonal antibody.
(1 Review)
(7 Publications)
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
GEMIN2 Antibody
(OTI4C11)
NBP2-45831
Western Blot: SIP1 Antibody (4C11) [NBP2-45831] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GEMIN2 .
Immunohistochemistry: GEMIN2 Antibody (4C11) [NBP2-45831] - SIP1 Antibody (4C11) [NBP2-45831] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: GEMIN2 Antibody (4C11) [NBP2-45831] - SIP1 Antibody (4C11) [NBP2-45831] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Species: Hu, Mu, Rt
Applications: IHC, WB
Vimentin Antibody
NB300-223
Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Analysis of HeLa cell culture. Antibody at 1:10,000 in green, and costained with mouse mAb to actin, dilution 1:500 in red. The blue is DAPI staining of nuclear DNA. The vimentin antibody stains the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.
Immunohistochemistry-Frozen: Vimentin Antibody [NB300-223] - 10 um Cryosections from PFA fixed murine brain acute slices stained with Vimentin antibody (1:600) and Alexa Fluor 488 anti chicken IgG. Antibody showed specific staining, also of blood vessels. IHC-Fr image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Human A7 cells. Cells were fixed with 10% formalin and permeabilized with 0.1% TritonX-100. After blocking, cells were incubated with primary antibody (1:600) at 4C O/N followed with Alexa Fluor 488 labeled secondary antibody and counterstained with DAPI and Phalloidin for nuclei and F-actin respectively. Imaged with Leica TCS SP5 confocal microscope. ICC/IF image submitted by a verified customer review.
(6 Reviews)
(55 Publications)
Species: Bv, Ca, Ch, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
Twist-1 Antibody
(10E4E6) - BSA Free
NBP2-37364
Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on HeLa cells with Twist-1 Antibody (10E4E6)NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550.
Flow (Intracellular): Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on HeLa cells with Twist-1 Antibody (10E4E6)NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550. Image from the DyLight 550 version of this antibody.
Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on SH-SY5Y cells with Twist-1 Antibody (10E4E6) NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550.
(1 Review)
(6 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-P, KD, WB
Snail Antibody
[Unconjugated]
AF3639
Western blot shows lysates of A549 human lung carcinoma cell line and JEG‑3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Snail Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3639) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Snail at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
A549 human lung carcinoma cell line was stained with Goat Anti-Human Snail Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3639, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Fluorescein-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0109). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Snail/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human Snail Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3639) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The E-Cadherin promoter was detected by standard PCR.
(12 Publications)
Species: Hu
Applications: ChIP, ICC, ICFlow, WB
SMN2 Antibody
(2B11-2A9)
H00006607-M01
Immunocytochemistry/Immunofluorescence: SMN2 Antibody (2B11-2A9) [H00006607-M01] - Analysis of monoclonal antibody to SMN2 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: SMN2 Antibody (2B11-2A9) [H00006607-M01] - Analysis of monoclonal antibody to SMN2 on formalin-fixed paraffin-embedded human heart tissue. Antibody concentration 1 ~ 10 ug/ml.
ELISA: SMN2 Antibody (2B11-2A9) [H00006607-M01] - Detection limit for recombinant GST tagged SMN2 is approximately 0.3ng/ml as a capture antibody.
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
SMN Antibody (2B1)
NB100-1936
Immunohistochemistry: SMN Antibody (2B1) [NB100-1936] - Analysis of SMN on mouse brain using NB100-1936.
Western Blot: SMN Antibody (2B1) [NB100-1936] - Analysis of SMN expression in 1) HeLa, 2) NTera2, 3) HepG2, 4) MCF7, 5) NIH 3T3, 6) PC12, and 7) COS7 whole cell lysates.
Immunohistochemistry: SMN Antibody (2B1) [NB100-1936] - CBs lose their colocalization with SMN nuclear foci in diabetic mice. Normal and pathological distribution of SMN proteins in sensory neurons. SMN proteins were found throughout the cytoplasm and as nuclear foci in control sensory neurons. SMN nuclear foci localized within CBs (coilin) in controls (upper panels, white arrows), whereas in the diabetic nucleus the numerous CBs were present but lost their colocalization with SMN nuclear foci (lower panels, yellow arrows). Arrowheads indicate sensory neurons magnified in the insets. Scale bars: 20 um, 10 um in insets. Image collected and cropped by CiteAb from the following publication (http://dmm.biologists.org/lookup/doi/10.1242/dmm.028225), licensed under a CC-BY licence.
(22 Publications)
Species: Hu, Mu, Pm, Rt, Xp, Ze
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, IP, WB
EDNRB/Endothelin R
Type B Antibody - BSA ...
NLS54
Immunohistochemistry-Paraffin: EDNRB/Endothelin R Type B Antibody [NLS54] - Brain, Dementia, Senile Plaque
Immunohistochemistry-Paraffin: EDNRB/Endothelin R Type B Antibody [NLS54] - Artery, smooth muscle
Immunohistochemistry-Paraffin: EDNRB/Endothelin R Type B Antibody [NLS54] - Brain, Alzheimer's senile plaque
(2 Publications)
Species: Ca, Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
GEMIN6 Antibody
H00079833-B01P
Immunocytochemistry/Immunofluorescence: GEMIN6 Antibody [H00079833-B01P] - Analysis of purified antibody to GEMIN6 on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: GEMIN6 Antibody [H00079833-B01P] - Analysis of GEMIN6 expression in transfected 293T cell line by GEMIN6 polyclonal antibody. Lane 1: GEMIN6 transfected lysate(18.37 KDa). Lane 2: Non-transfected lysate.
Western Blot: GEMIN6 Antibody [H00079833-B01P] - Analysis of GEMIN6 expression in HeLa.
Species: Hu
Applications: ICC/IF, WB
SNRPN Antibody
NBP2-20439
Immunohistochemistry-Paraffin: SNRPN Antibody [NBP2-20439] - SNRPN antibody detects SNRPN protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. SNRPN antibody dilution: 1:500.
Immunohistochemistry-Paraffin: SNRPN Antibody [NBP2-20439] - SNRPN antibody detects SNRPN protein at cytosol on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. SNRPN antibody dilution: 1:500.
Western Blot: SNRPN Antibody [NBP2-20439] - Sample (30 ug of whole cell lysate) A: JurKat 12% SDS PAGE gel, diluted at 1:1000.
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
MMP-2 [HRP]
MMP200
(88 Publications)
Species: Ca, Hu, Mu, Po, Rt
Applications: ELISA
TGF-beta 1
[Unconjugated]
7754-BH/CF
Recombinant human TGF-beta 1 (7754-BH/CF) inhibits recombinant mouse IL-4 induced proliferation in the HT-2 mouse T cell line. The ED50 for this effect is 0.04-0.2 ng/mL.
(21 Publications)
Species: Hu
Applications: BA
N-Cadherin
Antibody (13A9)
NBP1-48309
Flow (Intracellular): N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa with NBP1-48309 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody.
Flow Cytometry: N-Cadherin Antibody (13A9) [NBP1-48309] - An intracellular stain was performed on HeLa cells with NBP1-48309AF488 and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (13A9) [NBP1-48309] - HEK 293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti- at 5 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
(2 Reviews)
(37 Publications)
Species: Hu, Mu, Rt
Applications: Flow-IC, Flow, ICC, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
ZEB2 Antibody (2H5)
H00009839-M02
ZEB2 Antibody (2H5)
Species: Hu
Applications: WB, ELISA

Publications for ZEB2 Antibody (H00009839-M02)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Review for ZEB2 Antibody (H00009839-M02) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00009839-M02:
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Images Ratings Applications Species Date Details
Western Blot ZEB2 H00009839-M02
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reviewed by:
Verified Customer
 WB Human 06/30/2014
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Summary

ApplicationWestern Blot
Sample TestedHUVEC
SpeciesHuman
Lot11361-2H5

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Western Blot ZEB2 H00009839-M02
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 Verified Customer
06/30/2014
Application: WB
Species: Human
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Bioinformatics

Gene Symbol ZEB2
Entrez
Uniprot