ZEB1 Antibody

Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody [NBP2-20942] - ZEB1 antibody [N2C1], Internal detects ZEB1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ZEB1 protein stained by ZEB1 antibody [N2C1], Internal (NBP2-20942) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar = 10 um.

Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [N2C1], Internal (NBP2-20942) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody (NBP2-20942) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [N2C1], Internal (NBP2-20942) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-ZEB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to LAMC2 promotor.

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [N2C1], Internal (NBP2-20942) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

ZEB1 antibody immunoprecipitates ZEB1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of ZEB1 protein by 2 ug of ZEB1 antibody (NBP2-20942) 7.5% SDS-PAGE The immunoprecipitated ZEB1 protein was detected by ZEB1 antibody (NBP2-20942) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IP, ChIP, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ZEB1 Antibody Summary

• Description: Novus Biologicals Rabbit ZEB1 Antibody (NBP2-20942) is a polyclonal antibody validated for use in WB, ICC/IF, IP and ChIP. Anti-ZEB1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ZEB1. The exact sequence is proprietary.
• Localization: Nucleus
• Marker: Mesenchymal Cells Marker
• Predicted Species: Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZEB1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) Assay dependent
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockdown Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 124 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for ZEB1 Antibody

• AREB6
• AREB6MGC133261
• BZP
• delta-crystallin enhancer binding factor 1
• DELTAEF1
• FECD6
• Negative regulator of IL2
• NIL-2-A zinc finger protein
• NIL2A
• NIL-2-A
• posterior polymorphous corneal dystrophy 3
• PPCD3
• TCF8
• TCF-8
• TCF8BZP
• transcription factor 8 (represses interleukin 2 expression)
• Transcription factor 8
• ZEB
• ZEB1
• ZFHEP
• ZFHX1A
• zinc finger E-box binding homeobox 1
• zinc finger E-box-binding homeobox 1
• zinc finger homeodomain enhancer-binding protein

Background

ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZEB1 Antibody (NBP2-20942)(1)

Publication using NBP2-20942

• Deleuze V, Garcia L, Rouaisnel B et al. Efficient genome editing in erythroid cells unveils novel MYB target genes and regulatory functions iScience 2023-09-15 [PMID: 37670779]

FAQs for ZEB1 Antibody (NBP2-20942). (Showing 1 - 1 of 1 FAQ).

1. In looking at the Zeb1 antibodies on your website, the molecular weights of Zeb1 detected by several antibodies are different. Why is this?
   • ZEB1 is a rather large protein and undergoes a lot of post translational modifications such as phosphorylation and glycosylation (please see: UniProt P37275). The reason band patterns are different in the images may be due to sample type and how highly modified the ZEB1 protein is that the antibody is detecting. The more post translational modifications the higher you will detect the protein.

Bioinformatics

• Gene Symbol: ZEB1
• Uniprot:
  • P37275()