ZEB1 Antibody - BSA Free

Western Blot: ZEB1 Antibody [NBP1-77178] - HeLa cell lysate with ZEB1 antibody at 1ug/mL.

Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody - BSA Free [NBP1-77178] - Immunocytochemistry of ZEB1 in K562 cells with ZEB1 antibody at 2.5 u/ml.

Immunohistochemistry: ZEB1 Antibody - BSA Free [NBP1-77178] - Immunohistochemistry of ZEB1 in human liver tissue with ZEB1 antibody at 5 u/mL.

Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody - BSA Free [NBP1-77178] - Immunocytochemistry of ZEB1 in HeLa cells with ZEB1 antibody at 20 u/mL.

Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody - BSA Free [NBP1-77178] - Immunofluorescence of ZEB1 in Hela cells with ZEB1 antibody at 20 ug/mL.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ZEB1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ZEB1 Antibody - BSA Free (NBP1-77178) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide near the center of human ZEB1. The immunogen is located within amino acids 530 - 580 of ZEB1. Amino Acid Squence: HYDLKQPTQPPPLPA
• Marker: Mesenchymal Cells Marker
• Specificity: Multiple isoforms of ZEB1 are known to exist.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZEB1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1 ug/ml
• Theoretical MW: 130 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  ZEB1 Peptide (NBP1-77178PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for ZEB1 Antibody - BSA Free

• AREB6
• AREB6MGC133261
• BZP
• delta-crystallin enhancer binding factor 1
• DELTAEF1
• FECD6
• Negative regulator of IL2
• NIL-2-A zinc finger protein
• NIL2A
• NIL-2-A
• posterior polymorphous corneal dystrophy 3
• PPCD3
• TCF8
• TCF-8
• TCF8BZP
• transcription factor 8 (represses interleukin 2 expression)
• Transcription factor 8
• ZEB
• ZEB1
• ZFHEP
• ZFHX1A
• zinc finger E-box binding homeobox 1
• zinc finger E-box-binding homeobox 1
• zinc finger homeodomain enhancer-binding protein

Background

ZEB1, initially identified as the delta-crystallin enhancer binding protein delta EF1, is a DNA-binding protein that binds to a modified E-box sequence and has been implicated in postgastrulation embryogenesis. ZEB1 binds to the promoter of several hemapoietic genes, including interleukin-2, CD4, GATA-3, and alpha(4)-integrin, and mice in which ZEB1 has been targeted show thymic atrophy, and severe defects in lymphocyte differentiation. Recent evidence suggests that ZEB1 also regulates the accumulation of adipose tissue and may play a role in obesity. Mutations in this gene have been associated with late-onset Fuchs endothelial corneal dystrophy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for ZEB1 Antibody (NBP1-77178). (Showing 1 - 1 of 1 FAQ).

1. In looking at the Zeb1 antibodies on your website, the molecular weights of Zeb1 detected by several antibodies are different. Why is this?
   • ZEB1 is a rather large protein and undergoes a lot of post translational modifications such as phosphorylation and glycosylation (please see: UniProt P37275). The reason band patterns are different in the images may be due to sample type and how highly modified the ZEB1 protein is that the antibody is detecting. The more post translational modifications the higher you will detect the protein.

Bioinformatics

• Gene Symbol: ZEB1
• Uniprot:
  • CAI12550(Human)