ZEB1 Antibody

Images

 
Western Blot: ZEB1 Antibody [NBP1-68930] - Lanes: 1. 45ug capan1 cell lysate 2. 45 ug HPAF cell lysate Primary, Antibody Dilution: 1 : 1000 Secondary Antibody: Anti-Rabbit HRP Secondary, Antibody Dilution: 1 : 5000 Gene ...read more
Immunohistochemistry: ZEB1 Antibody [NBP1-68930] - Human Capan1 cells (Pancreatic cancer cell line) Primary Antibody Dilution: 1 : 300 Secondary Antibody: Anti-rabbit-AlexaFluor-488 Secondary Antibody Dilution: 1 : ...read more
Western Blot: ZEB1 Antibody [NBP1-68930] - Lane 1: Control, Lane 2: ZEB1 morphants. Upper panel: ZEB1 blot; Bottom panel: tubulin blot
Western Blot: ZEB1 Antibody [NBP1-68930] - 293T cells lysate, concentration 0.2-1 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ZEB1 Antibody Summary

Immunogen
Synthetic peptides corresponding to ZEB1 (zinc finger E-box binding homeobox 1) The peptide sequence was selected from the N terminal of ZEB1. Peptide sequence NPRRNNVTNYNTVVETNSDSDDEDKLHIVEEESVTDAADCEGVPEDDLPT.
Marker
Mesenchymal Cells Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZEB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry
Application Notes
This is a rabbit polyclonal antibody against ZEB1 and was validated on Western blot.
Theoretical MW
124 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NBP1-68930 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS & 2% Sucrose.
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for ZEB1 Antibody

  • AREB6
  • AREB6MGC133261
  • BZP
  • delta-crystallin enhancer binding factor 1
  • DELTAEF1
  • FECD6
  • Negative regulator of IL2
  • NIL-2-A zinc finger protein
  • NIL2A
  • NIL-2-A
  • posterior polymorphous corneal dystrophy 3
  • PPCD3
  • TCF8
  • TCF-8
  • TCF8BZP
  • transcription factor 8 (represses interleukin 2 expression)
  • Transcription factor 8
  • ZEB
  • ZEB1
  • ZFHEP
  • ZFHX1A
  • zinc finger E-box binding homeobox 1
  • zinc finger E-box-binding homeobox 1
  • zinc finger homeodomain enhancer-binding protein

Background

ZEB1 is a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ZEB1 Antibody (NBP1-68930) (0)

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Review for ZEB1 Antibody (NBP1-68930) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-68930:
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Images Ratings Applications Species Date Details
Western Blot ZEB1 NBP1-68930
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reviewed by:
Samantha Garcia
WB Other 11/05/2013
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Summary

ApplicationWestern Blot
Sample Testedzebrafish embryonic trunk at 3 days post fertilization
SpeciesOther
Lotqc31060
CommentsBands observed at 130 kDa

Blocking

Blocking DetailsTBS, 5% BSA, 1 hour room temperature

Primary Anitbody

Dilution Ratio1:500 TBS, 1% BSA, O.N. 4C

Secondary Antibody

Secondary Descriptionanti rabbit IgG peroxidase
Secondary Manufacturer Cat#sigma A6154
Secondary Concentration1:5000, TBS, 0,3% tween, 1 hour room temperature

Details

Detection Noteschemiluminescent, luminata Forte Western HRP substrate following manufacturer’s instructions, from 30 sec to 5 minutes

Comments

CommentsBands observed at 130 kDa

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ZEB1 Antibody (NBP1-68930). (Showing 1 - 2 of 2 FAQ).

  1. In looking at the Zeb1 antibodies on your website, the molecular weights of Zeb1 detected by several antibodies are different. Why is this?
    • ZEB1 is a rather large protein and undergoes a lot of post translational modifications such as phosphorylation and glycosylation (please see: UniProt P37275). The reason band patterns are different in the images may be due to sample type and how highly modified the ZEB1 protein is that the antibody is detecting. The more post translational modifications the higher you will detect the protein.
  2. I'm looking for an antibody that recognize zeb1 (zinc finger E-box binding homeobox 1) in zebrafish. In particular, I am interested in the detection by WB of zeb1a (in zebrafish there are two different isoforms). Do you have an antibody that is at least predicted to be reactive in zebrafish?
    • Our ZEB1 antibody #NBP1-68930 would be the best choice for you as its immunogen sequence is 100% identical to Zebrafish Zeb1. Besides WB, our customers have reported the workability of this antibody in ICC-IF application also. If you would be interested in testing this novel species or this novel application, please take a look at our Innovators Reward Program.

Secondary Antibodies

 

Isotype Controls

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Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition
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CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

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Recent Reviews

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Samantha Garcia
11/05/2013
Application: WB
Species: Other

Bioinformatics

Gene Symbol ZEB1
Entrez
Uniprot