Synthetic peptides corresponding to ZEB1 (zinc finger E-box binding homeobox 1) The peptide sequence was selected from the N terminal of ZEB1. Peptide sequence NPRRNNVTNYNTVVETNSDSDDEDKLHIVEEESVTDAADCEGVPEDDLPT.
Mesenchymal Cells Marker
Immunogen affinity purified
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Please see the vial label for concentration. If unlisted please contact technical services.
Immunogen affinity purified
Western Blot 1:100-1:2000
This is a rabbit polyclonal antibody against ZEB1 and was validated on Western blot.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 3 using NBP1-68930 in the following applications:
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
ZEB1 is a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for ZEB1 Antibody (NBP1-68930). (Showing 1 - 2 of 2 FAQ).
In looking at the Zeb1 antibodies on your website, the molecular weights of Zeb1 detected by several antibodies are different. Why is this?
ZEB1 is a rather large protein and undergoes a lot of post translational modifications such as phosphorylation and glycosylation (please see: UniProt P37275). The reason band patterns are different in the images may be due to sample type and how highly modified the ZEB1 protein is that the antibody is detecting. The more post translational modifications the higher you will detect the protein.
I'm looking for an antibody that recognize zeb1 (zinc finger E-box binding homeobox 1) in zebrafish. In particular, I am interested in the detection by WB of zeb1a (in zebrafish there are two different isoforms). Do you have an antibody that is at least predicted to be reactive in zebrafish?
Our ZEB1 antibody #NBP1-68930 would be the best choice for you as its immunogen sequence is 100% identical to Zebrafish Zeb1. Besides WB, our customers have reported the workability of this antibody in ICC-IF application also. If you would be interested in testing this novel species or this novel application, please take a look at our Innovators Reward Program.