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ZBTB7A/Pokemon Antibody

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 (1 Review)
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Western Blot: ZBTB7A/Pokemon Antibody [NBP2-23673] - Analysis of Pokemon in MCF7 cell lysate. Lane 1 shows pre-immune sera and lane 2 shows Pokemon antibody tested on MCF7 at 1:1000 dilution.
Western Blot: ZBTB7A/Pokemon Antibody [NBP2-23673] - Analysis of Pokemon in MCF7 cell lysate. Lane 1 shows pre-immune sera and lane 2 shows Pokemon antibody tested on MCF7 at 1:1000 dilution.
Immunohistochemistry-Paraffin: ZBTB7A/Pokemon Antibody [NBP2-23673] - Analysis of human skin using Pokemon antibody at 1:100.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, Ma-Op, Pm, Bv, Pm, EqSpecies Glossary
Applications WB, IHC, IHC-P, Simple Western (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ZBTB7A/Pokemon Antibody Summary

Immunogen
A portion of amino acid 1-50 of human Pokemon was used as the immunogen for this antibody.
Predicted Species
Chimpanzee (100%), Bovine (95%), Equine (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ZBTB7A
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100
Reviewed Applications
Read 1 Review rated 5
using
NBP2-23673 in the following applications:
Publications
Read Publication using
NBP2-23673 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (86%).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.1 ml crude whole Rabbit sera with 50% Glycerol
Preservative
0.025% Sodium Azide
Purity
Unpurified

Alternate Names for ZBTB7A/Pokemon Antibody

  • DKFZp547O146
  • Factor binding IST protein 1
  • Factor that binds to inducer of short transcripts protein 1
  • FBI-1
  • FBI-1POK erythroid myeloid ontogenic factor
  • FBI1TTF-I-interacting peptide 21
  • HIV-1 inducer of short transcripts binding protein
  • Leukemia/lymphoma-related factor
  • LRF
  • LRFHIV-1 1st-binding protein 1
  • lymphoma related factor
  • MGC99631
  • Pokemon
  • TIP21
  • ZBTB7A
  • ZBTB7zinc finger and BTB domain containing 7
  • zinc finger and BTB domain containing 7A
  • zinc finger and BTB domain containing 7A, HIV-1 inducer of short transcriptsbinding protein
  • zinc finger and BTB domain-containing protein 7A
  • Zinc finger protein 857A
  • ZNF857APOZ and Krueppel erythroid myeloid ontogenic factor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: p19ARF/CDKN2A Antibody [NB200-106] - Antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
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Western Blot: PLOD1 Antibody [NBP2-38770] - Analysis in human cell line U-87 MG.
Western Blot: PLOD1 Antibody [NBP2-38770] - SC65 directly interacts with lysyl-hydroxylase 1 (LH1). Western blot of primary calvarial osteoblast and skin fibroblast lysates from WT and Sc65KO 3 day-old mice (N = 2) showing significantly decreased levels of LH1 protein in Sc65KO samples. Densitometric quantification of LH1 protein normalized to beta-actin from the western blot shown above (*p<0.05; error bars represent SD). All experiments were performed at least 3 times. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pgen.1006002), licensed under a CC-BY licence.
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Western Blot: BRD2 Antibody [NBP1-30475] - Detection of Human and Mouse BRD2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody NBP1-30475 used for WB at 0.04 ug/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunocytochemistry/Immunofluorescence: BRD2 Antibody [NBP1-30475] - Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti- BRD2 used at a dilution of 1:100 (2 ug/mL) (left) and rabbit anti-BRD2 recombinant monoclonal used at a dilution of 1:20 (right). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 conjugated.
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Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Western Blot: DAND5 Antibody (2H3) [NBP2-01223] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAND5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAND5.
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Flow Cytometry: DAND5 Antibody (2H3) [NBP2-01223] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DAND5 antibody, and then analyzed by flow cytometry.
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Western Blot: SP1 Antibody [NB600-232] - Detection of Human SP1 by Western Blot. Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-SP1 antibody (NB600-232) used for WB at 0.04 ug/mL. Detection by chemiluminescence with an exposure time of 3 minutes.
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Western Blot: MID1 Antibody [NBP3-05061] - analysis of extracts of HeLa cells, using MID1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.
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Western Blot: MID1 Antibody [NBP3-05061] - Analysis of extracts of various cell lines, using MID1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit
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Publications for ZBTB7A/Pokemon Antibody (NBP2-23673)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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(1)
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Showing Publication 1 - 1 of 1.
Publication using NBP2-23673 Applications Species
Zhang Ji Yeon, Sui Lihua, Chen Jie, Gu Zhangru. Pokemon expression and clinical significance in ovarian cancer. Journal of Clinical Oncology 2014 Feb 01 (IHC-P, Human)

Details:
Normal ovary, borderline ovarian tumors and ovarian cancer: Fig 1, Tables 1, 2. 		IHC-P Human

Review for ZBTB7A/Pokemon Antibody (NBP2-23673) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-23673:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot ZBTB7A/Pokemon NBP2-23673
Enlarge 		5
reviewed by:
Anonymous
 WB Mouse 09/25/2020
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Summary

ApplicationWestern Blot
Sample TestedLiver
SpeciesMouse
Lot02152488a-01

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Western Blot ZBTB7A/Pokemon NBP2-23673
Enlarge
 Anonymous
09/25/2020
Application: WB
Species: Mouse
read this review

Bioinformatics

Gene Symbol ZBTB7A