ZAP70 [p Tyr494] Antibody - BSA Free

Immunoprecipitation: ZAP70 [p Tyr494] Antibody [NBP1-18898] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-ZAP70 antibody NB100-97826 was used at 3 mcg/mg lysate to immunoprecipitate ZAP70. The immunoprecipites were either treated with phosphatase (+) or mock (-) treated

Immunoprecipitation: ZAP70 [p Tyr494] Antibody [NBP1-18898] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide. Antibodies: Affinity purified rabbit anti-Phospho ZAP70 antibodies NBP1-18896 (for phosphorylation at Y319), NBP1-18897 (for phosphorylation at Y493), and NBP1-18898 (for phosphorylation at Y493/Y494) used for IP at 3 mcg/mg lysate.

• Reactivity: Hu
• Applications: IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ZAP70 [p Tyr494] Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ZAP70 [p Tyr494] Antibody - BSA Free (NBP1-18898) is a polyclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region including tyrosines 493 and 494 (when they are phosphorylated) of human zeta-chain (TCR) associated protein kinase 70kDa using the numbering given in entry NP_001070.2 (GeneID 7535).
• Modification: p Tyr494
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ZAP70
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
• Application Notes: It is not recommended for western analysis.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZAP70 [p Tyr494] Antibody - BSA Free

• 70 kDa zeta-associated protein
• EC 2.7.10
• EC 2.7.10.2
• mrtle
• mur
• SRK
• SRKFLJ17679
• STD
• STDFLJ17670
• Syk-related tyrosine kinase
• tyrosine-protein kinase ZAP-70
• TZK
• ZAP70
• ZAP-70
• zeta-chain (TCR) associated protein kinase (70 kD)
• zeta-chain (TCR) associated protein kinase 70kDa
• zeta-chain associated protein kinase, 70kD

Background

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZAP70
• Entrez:
  • 7535(Human)
• Uniprot:
  • NP_001070.2(Human)