ZAP70 Antibody (ZAP70/4040R)

Immunohistochemistry-Paraffin: ZAP70 Antibody (ZAP70/4040R) [NBP3-07223] - Formalin-fixed, paraffin-embedded human tonsil stained with ZAP70 Recombinant Rabbit Monoclonal Antibody (ZAP70/4040R).

Immunohistochemistry-Paraffin: ZAP70 Antibody (ZAP70/4040R) [NBP3-07223] - Formalin-fixed, paraffin-embedded human tonsil stained with ZAP70 Recombinant Rabbit Monoclonal Antibody (ZAP70/4040R).

• Reactivity: Hu
• Applications: ELISA, IHC
• Clone: ZAP70/4040R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

ZAP70 Antibody (ZAP70/4040R) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 1 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08968)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide corresponding to residues withinaa200-350 of ZAP70 was used as an immunogen. (Uniprot: P43403 )
• Localization: Cell surface and cytoplasm
• Marker: Chronic Lymphocytic Leukemia Marker
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ZAP70
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 2-4 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
  Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 1 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for ZAP70 Antibody (ZAP70/4040R)

• 70 kDa zeta-associated protein
• EC 2.7.10
• EC 2.7.10.2
• mrtle
• mur
• SRK
• SRKFLJ17679
• STD
• STDFLJ17670
• Syk-related tyrosine kinase
• tyrosine-protein kinase ZAP-70
• TZK
• ZAP70
• ZAP-70
• zeta-chain (TCR) associated protein kinase (70 kD)
• zeta-chain (TCR) associated protein kinase 70kDa
• zeta-chain associated protein kinase, 70kD

Background

Zap-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation (1). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with activation of ZAP-70, a critical event in T cell activation (2). Following TCR engagement, ZAP-70 is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family tyrosine kinase, Lck. Phosphorylation of Tyr319 is required for full activation and increased positive downstream regulation by ZAP-70 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZAP70
• Uniprot:
  • P43403()
  • P43403()