ZAP70 Antibody (RM408)

Western Blot: ZAP70 Antibody (RM408) [NBP2-89104] - Western Blot of Jurkat cell lysate using Anti-ZAP70 rabbit monoclonal antibody (Clone RM408) at a 1:500 dilution.

Immunohistochemistry-Paraffin: ZAP70 Antibody (RM408) [NBP2-89104] - Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using Anti-ZAP70 Rabbit Monoclonal Antibody (Clone RM408) at a 1:200 dilution.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: RM408
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

ZAP70 Antibody (RM408) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: A peptide corresponding to residues near the N-terminus of human ZAP70
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ZAP70
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 50% Glycerol/PBS, 1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for ZAP70 Antibody (RM408)

• 70 kDa zeta-associated protein
• EC 2.7.10
• EC 2.7.10.2
• mrtle
• mur
• SRK
• SRKFLJ17679
• STD
• STDFLJ17670
• Syk-related tyrosine kinase
• tyrosine-protein kinase ZAP-70
• TZK
• ZAP70
• ZAP-70
• zeta-chain (TCR) associated protein kinase (70 kD)
• zeta-chain (TCR) associated protein kinase 70kDa
• zeta-chain associated protein kinase, 70kD

Background

Zap-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation (1). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with activation of ZAP-70, a critical event in T cell activation (2). Following TCR engagement, ZAP-70 is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family tyrosine kinase, Lck. Phosphorylation of Tyr319 is required for full activation and increased positive downstream regulation by ZAP-70 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ZAP70