ZAG Antibody Pair [Biotin]

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin
• Suitable Sample Type: Cell Culture Supernatant, Plasma, Serum
• Standard Curve Range: 0.0156 - 1 ng/ml
• Sensitivity: 100 pg/ml

ZAG Antibody Pair [Biotin] Summary

• Description: Sandwich ELISA for the quantitative determination of Human ZAG
• Specificity: Detects human ZAG. Does not detect mouse ZAG.
• Standard Curve Range: 0.0156 - 1 ng/ml
• Sensitivity: 100 pg/ml
• Assay Type: Sandwich ELISA
• Sample Volume: 100 ul
• Gene: AZGP1

Applications/Dilutions

• Dilutions:
  • Sandwich ELISA
• Application Notes: 1. Dilute the desired amount of Coating Antibody (COAT) (1mg/ml) (mouse monoclonal antibody) to 2ug/ml in PBS without carrier protein and use it fresh.
  2. Dilute the desired amount of Detection Antibody (DET) (0.1mg/ml) (biotinylated mouse monoclonal antibody) to 0.1ug/ml in ELISA Buffer and use it fresh.
  3. Reconstitute the Streptavidin-HRP (STREP) with 100ul ELISA Buffer. After reconstitution, prepare aliquots and store the reconstituted STREP at -20C. Avoid freeze/thaw cycles. Dilute the reconstituted Streptavidin-HRP to the working concentration by adding 5ul in 10ml of ELISA Buffer (1:2000).
  4. Reconstitute the Standard Protein (STD) (recombinant human ZAG) with 100ul ELISA Buffer to obtain a concentration of 10ug/ml. After reconstitution, prepare aliquots and store the reconstituted standard at -20C. Avoid freeze/thaw cycles. Suggested standard points are 1ng/ml, 0.5ng/ml, 0.25 ng/ml, 0.125 ng/ml, 0.062 ng/ml, 0.031 ng/ml, 0.0156ng/ml and 0 ng/ml.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Alternate Names for ZAG Antibody Pair [Biotin]

• alpha-2-glycoprotein 1, zinc
• alpha-2-glycoprotein 1, zinc-binding
• AZGP1
• ZA2G
• ZAG
• ZAGzn-alpha-2-GP
• zinc
• zinc-alpha-2-glycoprotein
• zn-alpha-2-glycoprotein
• ZNGP1

Background

Zinc Alpha 2 Glycoprotein is a versatile protein which has several important functions such as fertilization and lipid metabolism. It has also been associated with causing fat loss with some cancer types. This protein is known to have interaction with ITGAV, USP53, STK11, PRKAB2, and PRKAB1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: AZGP1