YY1 Antibody

Western Blot: YY1 Antibody [NBP2-20933] - 30 ug 293T whole cell lysate/extract10 % SDS-PAGE YY1 antibody dilution: 1:1000.

Immunoprecipitation: YY1 Antibody [NBP2-20933] - Sample: 1000 ug HeLa whole cell lysate/extract A. 50 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of YY1protein by 2 ug of YY1 antibody 10% SDS-PAGE The immunoprecipitated YY1 protein was detected by YY1 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.

YY1 antibody detects YY1 protein by Western blot analysis.A. 30 ug 293T whole cell lysate/extract10 % SDS-PAGEYY1 antibody (NBP2-20933) dilution: 1:1000

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

YY1 Antibody Summary

• Description: Novus Biologicals Rabbit YY1 Antibody (NBP2-20933) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human YY1. The exact sequence is proprietary.
• Localization: Nucleus matrix
• Predicted Species: Mouse (98%), Rat (98%), Bovine (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: YY1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (80%), Chicken (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for YY1 Antibody

• Delta transcription factor
• DELTA
• INO80SYY-1
• NF-E1
• NF-E1INO80 complex subunit S
• transcriptional repressor protein YY1
• UCRBP
• Yin and Yang 1 protein
• Yin and yang 1
• YIN-YANG-1
• YY1 transcription factor
• YY1

Background

YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: YY1
• Uniprot:
  • P25490()