XRN2 Antibody

Western Blot: XRN2 Antibody [NB100-57541] - Detection of human XRN2 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN2 antibody NB100-57541 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry-Paraffin: XRN2 Antibody [NB100-57541] - Sample: FFPE sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-XRN2 (NB100-57541). Detection: DAB

Western Blot: XRN2 Antibody [NB100-57541] - Detection of mouse XRN2 by western blot. Samples: Whole cell lysate (15 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XRN2 antibody NB100-57541 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry-Paraffin: XRN2 Antibody [NB100-57541] - Sample: FFPE sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-XRN2 (NB100-57541). Detection: DAB

Immunoprecipitation: XRN2 Antibody [NB100-57541] - Detection of human XRN2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-XRN2 antibody NB100-57541 used for IP at 3 ug per reaction. XRN2 was also immunoprecipitated by another rabbit anti-XRN2 antibody. For blotting immunoprecipitated XRN2, NB100-57541 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

XRN2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 900 and 950 human 5'-3' exoribonuclease 2 using the numbering given in entry NP_036387.2 (GeneID 22803).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: XRN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:10000 - 1:25000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for XRN2 Antibody

• 5'-3' exoribonuclease 2
• Dhm1-like protein (mouse homolog)
• DHM1-like protein
• DHP protein
• EC 3.1.13
• EC 3.1.13.-

Background

XRN2 is a 5'-to-3' exoribonuclease involved in 3'-end mRNA processing. XRN2 is important for transcription termination and is responsible for degrading the downstream 3'-end-cleaved RNA. Recent studies show that XRN2 is recruited to 3'-end processing machinery by p54nrb/PSF. Alternate names for XRN2 include 5'-3' exoribonuclease 2, DHM1-like protein, and DHP protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for XRN2 Antibody (NB100-57541)(2)

Publications using NB100-57541

• Cottrell KA, Ryu S, Pierce JR et Al. Induction of Viral Mimicry Upon Loss of DHX9 and ADAR1 in Breast Cancer Cells Cancer Res Commun 2024-04-01 [PMID: 38530197]
• Barnhart MD, Moon SL, Emch AW et al. Changes in Cellular mRNA Stability, Splicing, and Polyadenylation through HuR Protein Sequestration by a Cytoplasmic RNA Virus. Cell Rep. 2013-11-06 [PMID: 24210824] (WB, Human)

Review for XRN2 Antibody (NB100-57541) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Stephanie Moon WB Human 01/07/2014

Summary

• Application: Western Blot
• Species: Human

Bioinformatics

• Gene Symbol: XRN2
• Entrez:
  • 22803(Human)
• Uniprot:
  • Q9H0D6(Human)