XPV/DNA polymerase eta Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: XPV/DNA polymerase eta Antibody [NBP1-87165] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: XPV/DNA polymerase eta Antibody [NBP1-87165] - Staining of human lymph node shows moderate cytoplasmic and nuclear positivity in germinal center cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

XPV/DNA polymerase eta Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit XPV/DNA polymerase eta Antibody - BSA Free (NBP1-87165) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: PPLTMLFLCATKFSASAPSSSTDITSFLSSDPSSLPKVPVTSSEAKTQGSGPAVTATKKATTSLESFFQKAAERQKVKEASLSSLTAPTQAPMSNSPSKPSLPFQTSQSTGTE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  XPV/DNA polymerase eta Protein (NBP1-87165PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for XPV/DNA polymerase eta Antibody - BSA Free

• DNA polymerase eta
• DNA Polymerase, eta
• EC 2.7.7.7
• POLH
• polymerase (DNA directed), eta
• RAD30 homolog A
• RAD30
• RAD30A
• RAD30AFLJ16395
• Xeroderma pigmentosum variant type protein
• XPV
• XP-V
• XPVFLJ21978

Background

DNA polymerase eta is specifically involved in DNA repair. It plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. It also plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. It targets POLI to replication foci (referenced from swissprot).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLH