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XPF Antibody (XPF 3F2/3) [FITC]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, ICC/IF
Clone
XPF 3F2/3
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP1-27881AF405
Alexa Fluor 488
NBP1-27881AF488
Alexa Fluor 647
NBP1-27881AF647
Alexa Fluor 700
NBP1-27881AF700
Biotin
NBP1-27881B
DyLight 350
NBP1-27881UV
DyLight 405
NBP1-27881V
DyLight 488
NBP1-27881G
DyLight 550
NBP1-27881R
DyLight 650
NBP1-27881C
DyLight 680
NBP1-27881FR
DyLight 755
NBP1-27881IR
HRP
NBP1-27881H
Janelia Fluor 549
NBP1-27881JF549-0.2ml
Janelia Fluor 646
NBP1-27881JF646-0.2ml
Unconjugated
NBP1-27881
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XPF Antibody (XPF 3F2/3) [FITC] Summary

Immunogen
A recombinant His-tagged fragment of human XPF protein produced in E. coli
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ERCC4
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for XPF Antibody (XPF 3F2/3) [FITC]

  • DNA excision repair protein ERCC-4
  • DNA repair protein complementing XP-F cells
  • EC 3.1
  • ERCC11
  • ERCC4
  • excision repair cross-complementing rodent repair deficiency, complementationgroup 4
  • xeroderma pigmentosum, complementation group F
  • XPF
  • XPFcomplementing defective, in Chinese hamster

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: ERCC1 Antibody (8F1) [NB500-704] - Analysis of ERCC1 in human non-small cell lung cancer. Image courtesy of product review submitted by Alex Soltermann.
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(15 Publications)
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XPG Antibody
NB100-74611
Western Blot: XPG Antibody [NB100-74611] - Detection of Human ERCC5/XPG on HeLa whole cell lysate using Nb100-74611. ERCC5/XPG was also immunoprecipitated by rabbit anti-ERCC5/XPG antibody NB100-74612.
Immunohistochemistry: XPG Antibody [NB100-74611] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ERCC5/XPG used at a dilution of 1:200 (1ug/ml). Detection: DAB
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XPD Antibody
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Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, CH-1 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat XPD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3296) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for XPD at approximately 100 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
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XPC Antibody (3.26)
NB100-477
Western Blot: XPC Antibody (3.26) [NB100-477] - Whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with XPC antibody [3.26] diluted at 1:500.
Immunocytochemistry/Immunofluorescence: XPC Antibody (3.26) [NB100-477] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: XPC protein stained by XPC antibody [3.26] diluted at 1:100.Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.
Western Blot: XPC Antibody (3.26) [NB100-477] - HeLa whole cell lysate detected using rabbit polyclonal.
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(93A535)
NB120-13600
Western Blot: Rad9 Antibody (93A535) [NB120-13600] - Western blot analysis of RAD9 recombinant protein using RAD9 antibody at 2 ug/ml. Human tissue TMA was used for this test.
Immunohistochemistry-Paraffin: Rad9 Antibody (93A535) [NB120-13600] - Formalin-fixed, paraffin-embedded human prostate adenocarcinoma stained with RAD9 antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
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HUS1 Antibody
NBP1-33589
Western Blot: HUS1 Antibody [NBP1-33589] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: HUS1 Antibody [NBP1-33589] - Paraffin-embedded CA922 xenograft, using antibody at 1:500 dilution.
Western Blot: HUS1 Antibody [NBP1-33589] - 30 ug C8D30 whole cell lysate/extract 12 % SDS-PAGE gel, antibody dilution 1:1000.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
XPA Antibody
NB100-93321
Western Blot: XPA Antibody [NB100-93321] - Detection of human XPA by western blot. Samples: Whole cell lysate (50 ug) from HeLa, K-562, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XPA antibody NB100-93321 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry: XPA Antibody [NB100-93321] - Detection of human XPA by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- XPA (NB100-93321). Detection: DAB.
Western Blot: XPA Antibody [NB100-93321] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-93321 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate
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RAD52 Antibody
(5H9)
NBP1-41208
Western Blot: RAD52 Antibody (5H9) [NBP1-41208] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Rad52 antibody [5H9] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Western Blot: RAD52 Antibody (5H9) [NBP1-41208] - Western blot experiment using Rad52 antibody to detect human Rad52 protein expression in HeLa whole cell lysate.
Species: Hu
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PCNA Antibody
(PC10)
NB500-106
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LXR beta/NR1H2
Antibody
NB100-74457
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XPB Antibody
NB100-61060
Western Blot: XPB Antibody [NB100-61060] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiERCC3 antibody used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
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FEN-1 Antibody
(4E7)
NB100-150
Western Blot: FEN-1 Antibody (4E7) [NB100-150] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with FEN1 antibody diluted at 1:500.
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Antibody
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Rad51 Antibody
(14B4)
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Recombinant Human
CSB Protein
H00002074-Q01
12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
Chk1 Antibody
NB100-464
Western Blot: Chk1 Antibody [NB100-464] - Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TMCK cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody NB100-464 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: Chk1 Antibody [NB100-464] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHK1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: Chk1 Antibody [NB100-464] - Detection of human Chk1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody NB100-464 (lot 2) used for IP at 6 ug/mg lysate. Chk1 was also immunoprecipitated by a previous lot (lot 1) of this antibody. For blotting immunoprecipitated Chk1, NB100-464 (lot 2) was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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XRCC1 Antibody
NBP1-87154
Western Blot: XRCC1 Antibody [NBP1-87154] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented
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(MTA30 2G10/3)
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ATR Antibody (2B5)
NB100-308
Western Blot: ATR Antibody (2B5) [NB100-308] - Human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: ATR Antibody (2B5) [NB100-308] - Analysis of HeLa, using antibody at 1:100 dilution.
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XRCC3 Antibody
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Western Blot: XRCC3 Antibody [NB100-165] - (A) Human XRCC3 (hXRCC3) expression was down regulated by siRNA in human transofrmed fibroblasts. (B) XRCC3 was inactivated in human colon cancer cells by gene targeting [Yoshihara et all., 2004, EMBO J., 23 (3), 670-680]. hXRCC3 was detected by WB using XRCC3 antibody (NB100-165). The star indicates non-specific cross-reactivity of the antibody. Image provided by confirmed customer review.
Western Blot: XRCC3 Antibody [NB100-165] - Detection of XRCC3 (38 kDa) from HeLa cell extract using NB100-165 (1:2500). Lanes 1 and 2 contain HTC116 and Hela whole cell extracts, respectively.
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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP1-27881AF405
Alexa Fluor 488 NBP1-27881AF488
Alexa Fluor 647 NBP1-27881AF647
Alexa Fluor 700 NBP1-27881AF700
Biotin NBP1-27881B
DyLight 350 NBP1-27881UV
DyLight 405 NBP1-27881V
DyLight 488 NBP1-27881G
DyLight 550 NBP1-27881R
DyLight 650 NBP1-27881C
DyLight 680 NBP1-27881FR
DyLight 755 NBP1-27881IR
FITC NBP1-27881F
HRP NBP1-27881H
Janelia Fluor 549 NBP1-27881JF549
Janelia Fluor 646 NBP1-27881JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol ERCC4
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