XPE/DDB2 Antibody (4Y2G5)

Western Blot: XPE/DDB2 Antibody (4Y2G5) [NBP3-15414] - Western blot analysis of extracts of various cell lines, using XPE/DDB2 Rabbit mAb (NBP3-15414) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Western Blot: XPE/DDB2 Antibody (4Y2G5) [NBP3-15414] - Western blot analysis of extracts of Mouse lung, using XPE/DDB2 Rabbit mAb (NBP3-15414) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clone: 4Y2G5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

XPE/DDB2 Antibody (4Y2G5) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 220-427 of human XPE/DDB2 (Q92466). DNVGNVILLNMDGKELWNLRMHKKKVTHVALNPCCDWFLATASVDQTVKIWDLRQVRGKASFLYSLPHRHPVNAACFSPDGARLLTTDQKSEIRVYSASQWDCPLGLIPHPHRHFQHLTPIKAAWHPRYNLIVVGRYPDPNFKSCTPYELRTIDVFDGNSGKMMCQLYDPESSGISSLNEFNPMGDTLASAMGYHILIWSQEEARTRK
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DDB2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • Western Blot 1:1000 - 1:2000
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for XPE/DDB2 Antibody (4Y2G5)

• damage-specific DNA binding protein 2 (48kD)
• damage-specific DNA binding protein 2, 48kDa
• Damage-specific DNA-binding protein 2
• DDB p48 subunit
• DDB2
• DDBB
• DNA damage-binding protein 2
• FLJ34321
• UV-damaged DNA-binding protein 2
• UV-DDB 2
• UV-DDB2
• xeroderma pigmentosum group E protein
• XP5
• XPE/DDB2

Background

DDB2, also known as DNA damage-binding protein 2, consists of five isoforms of sizes 47.9 kDa, 26.7 kDa, 17.4 kDa, 40.7 kDa, and 26.8 kDa and is involved in repairing DNA damaged by ultraviolet light. Current research is being conducted on disorders and diseases including immunodeficiency, hepatitis, multiple sclerosis, lung cancer, ovarian cancer, skin cancer, and autosomal recessive disease. The protein has been linked to DNA repair and protein stability pathways where it interacts with CUL4A, DDB1, COPS5, ABL1, and E2F1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDB2