XIAP Antibody (117320) [Unconjugated]

Images

 
Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and L-929 mouse fibrosarcoma cell line. PVDF membrane was probed with 2 µg/mL Mouse Anti-Human/Mouse XIAP Monoclonal Antibody (Catalog # MAB822) ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and XIAP knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, KO
Clone
117320
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Formulation Size Price

XIAP Antibody (117320) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human XIAP
Met1-Ser497
Accession # P98170
Specificity
Detects human and mouse XIAP in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) cIAP-1 or rhcIAP-2 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
XIAP
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Knockout Validated
Reviewed Applications
Read 1 Review rated 3
using
MAB822 in the following applications:

Publications
Read Publications using
MAB822 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for XIAP Antibody (117320) [Unconjugated]

  • API3X-linked inhibitor of apoptosis protein
  • apoptosis inhibitor 3
  • baculoviral IAP repeat-containing 4
  • baculoviral IAP repeat-containing protein 4
  • BIRC4
  • BIRC4XLP2
  • E3 ubiquitin-protein ligase XIAP
  • EC 6.3.2.-
  • hIAP3
  • hIAP-3
  • hILP
  • IAP3
  • IAP-3
  • IAP-like protein
  • ILP
  • ILP1
  • Inhibitor of apoptosis protein 3
  • MIHA
  • XIAP
  • X-linked IAP
  • X-linked inhibitor of apoptosis

Background

XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the apoptosis (IAP) family of proteins that inhibit caspases. The BIR2 domain of XIAP inhibits Caspase-3 and Caspase-7. The ability of XIAP to inhibit caspases is prevented by SMAC/Diablo through binding to XIAP-BIR2 and -BIR3 domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for XIAP Antibody (MAB822)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(5)
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Filter By Species
Human
(3)
Mouse
(2)
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Showing Publications 1 - 5 of 5.
Publications using MAB822 Applications Species
S Gradzka, OS Thomas, O Kretz, A Haimovici, L Vasilikos, WW Wong, G Häcker, IE Gentle Inhibitor of apoptosis proteins are required for effective fusion of autophagosomes with lysosomes Cell Death Dis, 2018;9(5):529. 2018 [PMID: 29743550] (Western Blot, Mouse) Western Blot Mouse
A Dey, X Xiong, A Crim, SKD Dwivedi, S Banerjee M, P Mukherjee, L Cao, N Sydorenko, R Baiazitov, YC Moon, M Dumble, T Davis, R Bhattachar Evaluating the mechanism and therapeutic potential of PTC-028, a novel inhibitor of BMI-1 function in ovarian cancer Mol. Cancer Ther., 2017;0(0):. 2017 [PMID: 29158468] (Western Blot, Human) Western Blot Human
Umemori Y, Kuribayashi K, Nirasawa S, Kondoh T, Tanaka M, Kobayashi D, Watanabe N Protein kinase C zeta regulates survivin expression and inhibits apoptosis in colon cancer. Int J Oncol, 2014;45(3):1043-50. 2014 [PMID: 24920238] (Western Blot, Human) Western Blot Human
Matte I, Lane D, Boivin M, Rancourt C, Piche A MUC16 mucin (CA125) attenuates TRAIL-induced apoptosis by decreasing TRAIL receptor R2 expression and increasing c-FLIP expression. BMC Cancer, 2014;14(0):234. 2014 [PMID: 24690311] (Western Blot, Human) Western Blot Human
Schoenmann Z, Assa-Kunik E, Tiomny S, Minis A, Haklai-Topper L, Arama E, Yaron A Axonal degeneration is regulated by the apoptotic machinery or a NAD+-sensitive pathway in insects and mammals. J. Neurosci., 2010;30(18):6375-86. 2010 [PMID: 20445064] (Western Blot, Mouse) Western Blot Mouse

Review for XIAP Antibody (MAB822) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB822:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  3
reviewed by:
Verified Customer
WB Human 02/25/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 20074295
SpeciesHuman

Blocking

Blocking DetailsSee PMID 20074295

Details

Detection NotesSee PMID 20074295

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 20074295

Product General Protocols

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FAQs for XIAP Antibody (MAB822) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for XIAP Antibody (MAB822)

Discover related pathways, diseases and genes to XIAP Antibody (MAB822). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for XIAP Antibody (MAB822)

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Pathways for XIAP Antibody (MAB822)

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Learn more about PTMs related to XIAP Antibody (MAB822).

Blogs on XIAP.

The Ins and Outs of Survivin
By Rachel M.A. Linger, Ph.D.What is survivin?Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of...  Read full blog post.

Apoptosis and Necroptosis Part II: Inhibitors of apoptosis proteins (IAPs); Key regulators of the balance between necroptosis, apoptosis and survival
In the first installment of this two-part blog post titled "Apoptosis and Necroptosis: Important factors to identify both types of programmed cell death", the mechanisms by which cell death occurs and ways to identify these pathways were...  Read full blog post.

Caspase 9 - an important apoptosis marker
Caspases are essential mediators of programmed cell death and are needed for both the induction of apoptosis as well as for aiding the degradation of cellular structures. Initiator caspases (such as Caspase-9) sense and respond to various signals i...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

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Recent Reviews

3
5
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4
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3
1
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1
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Verified Customer
02/25/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol XIAP
Uniprot