Xanthine Oxidase Activity Assay Kit (Colorimetric) Summary
Description |
Detection instrument: Microplate reader (540 - 560 nm, optimum wavelength: 550 nm) Assay time: 35 min |
Standard Curve Range |
0.067 - 39.30 U/L |
Sensitivity |
0.067 U/L |
Inter-Assay |
%CV < 9.9 |
Intra-Assay |
%CV < 3 |
Spike Recovery |
Average recovery rate: 99% |
Kit Type |
Activity Assay Kit (Colorimetric) |
Gene |
XDH |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Microplate
- Plate Sealer
- Reagent 1: Buffer Solution
- Reagent 2: Substrate Solution
- Reagent 3: Enzymatic Reagent
- Reagent 4: Chromogenic Agent 1
- Reagent 5: Chromogenic Agent 2
- Reagent 6: 1 mmol/L Standard Solution
|
Alternate Names for Xanthine Oxidase Activity Assay Kit (Colorimetric)
Background
Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The enzyme is a homodimer. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. (provided by RefSeq)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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