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XAF1 Antibody

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Western Blot: XAF1 Antibody [NB100-56355] - analysis of XAF1 in human liver lysate using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: XAF1 Antibody [NB100-56355] - XAF1 was detected in immersion fixed paraffin-embedded sections of human appendix using Rabbit Anti-Human XAF1 polyclonal Antibody (Catalog # NB100-56355) at ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

XAF1 Antibody Summary

Immunogen
Amino acids 201-218 (NQTSTMEKDVRPKTRSIN) and 301-317 (QLDLEKERYYKFKRFHF) of human XAF1, GenBank no. CAA68030.1, were used as immunogen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
XAF1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1-2 ug/ml
Application Notes
In human liver, a 34 kDa band should be observed.
Control
Human Liver Whole Tissue Lysate (Adult Whole Normal)
XAF1 Overexpression Lysate
Publications
Read Publications using
NB100-56355 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for XAF1 Antibody

  • BIRC4-binding protein
  • BIRC4BPBIRC4 binding protein
  • HSXIAPAF1
  • XIAP associated factor 1
  • XIAPAF1XIAP-associated factor 1

Background

The inhibitors of apoptosis (IAP) proteins prevent cell death both in vitro and in vivo by virtue of inhibition of distinct caspases. The X-linked Inhibitor of Apoptosis, XIAP, is a key member of the IAP family of proteins. The caspase inhibiting activity of XIAP is negatively regulated by at least two XIAP-interacting proteins, XIAP-associated factor 1 (XAF1) and Smac/DIABLO. XAF1 binds to XIAP and antagonizes caspase inhibitory activities of XIAP. Expression of XAF1 triggers a redistribution of XIAP from the cytosol to the nucleus. XAF1 is ubiquitously expressed in normal tissues, but is present at low or undetectable levels in many different cancer cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for XAF1 Antibody (NB100-56355)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IF/IHC, IHC-P, WB.


Filter By Application
IF/IHC
(1)
IHC-P
(1)
WB
(2)
All Applications
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Human
(1)
Mouse
(2)
All Species

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol XAF1
Entrez