XAF1 Antibody

Western Blot: XAF1 Antibody [NB100-56355] - analysis of XAF1 in human liver lysate using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: XAF1 Antibody [NB100-56355] - XAF1 was detected in immersion fixed paraffin-embedded sections of human appendix using Rabbit Anti-Human XAF1 polyclonal Antibody (Catalog # NB100-56355) at 1:100 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sperm cells.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

XAF1 Antibody Summary

• Immunogen: Amino acids 201-218 (NQTSTMEKDVRPKTRSIN) and 301-317 (QLDLEKERYYKFKRFHF) of human XAF1, GenBank no. CAA68030.1, were used as immunogen.
• Specificity: In human liver, a 34 kDa band should be observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: XAF1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1-2 ug/ml
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)
  XAF1 Overexpression Lysate

Reactivity Notes

Cross reacts with Human and Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for XAF1 Antibody

• BIRC4-binding protein
• BIRC4BPBIRC4 binding protein
• HSXIAPAF1
• XIAP associated factor 1
• XIAPAF1XIAP-associated factor 1

Background

The inhibitors of apoptosis (IAP) proteins prevent cell death both in vitro and in vivo by virtue of inhibition of distinct caspases. The X-linked Inhibitor of Apoptosis, XIAP, is a key member of the IAP family of proteins. The caspase inhibiting activity of XIAP is negatively regulated by at least two XIAP-interacting proteins, XIAP-associated factor 1 (XAF1) and Smac/DIABLO. XAF1 binds to XIAP and antagonizes caspase inhibitory activities of XIAP. Expression of XAF1 triggers a redistribution of XIAP from the cytosol to the nucleus. XAF1 is ubiquitously expressed in normal tissues, but is present at low or undetectable levels in many different cancer cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for XAF1 Antibody (NB100-56355)(6)

Publications using NB100-56355

• Zhu C, Li B, Frontzek K et al. SARM1 deficiency up-regulates XAF1, promotes neuronal apoptosis, and accelerates prion disease J. Exp. Med. 2019-04-01 [PMID: 30842236] (WB, Mouse)
• Lunardi A, Ala U, Epping MT et al. A co-clinical approach identifies mechanisms and potential therapies for androgen deprivation resistance in prostate cancer. Nat Genet. 2013-07-01 [PMID: 23727860]
• Kempkensteffen Carsten, Fritzsche Florian Rudolf, Johannsen Manfred et al. Down-regulation of the pro-apoptotic XIAP associated factor-1 (XAF1) during progression of clear-cell renal cancer. BMC Cancer. 2009-01-01 [PMID: 19664236] (IF/IHC, Human)
• Straszewski-Chavez SL, Visintin IP, Karassina N et al. XAF1 mediates tumor necrosis factor-alpha-induced apoptosis and X-linked inhibitor of apoptosis cleavage by acting through the mitochondrial pathway. J Biol Chem. 2007-04-27 [PMID: 17329253]
• Xia Y, Novak R, Lewis J et al. Xaf1 can cooperate with TNFalpha in the induction of apoptosis, independently of interaction with XIAP. Mol Cell Biochem. 2006-06-01 [PMID: 16432762] (WB)
• Wang X, Zhu C, Wang X et al. X-linked inhibitor of apoptosis (XIAP) protein protects against caspase activation and tissue loss after neonatal hypoxia-ischemia. Neurobiol Dis. 2004-06-01 [PMID: 15207275] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: XAF1
• Entrez:
  • 54739(Human)