WWP1 Antibody

Western Blot: WWP1 Antibody [NBP3-23229] - Western blot analysis of WWP1 expression in adult mouse liver (lane 1) and human prostate adenocarcinoma (PC3) cells (lane 2). The blot was probed with anti-WWP1 (NBP3-23229) at 1:1000.

• Reactivity: Hu, Mu, Rt, Ch
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

WWP1 Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: WWP1 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the N-terminus of human WWP1. This sequence is highly conserved in rat and mouse WWP1, and has low homology to other NEDD4-like family members. [UniProt# Q9H0M0]
• Specificity: The antibody detects a 110 kDa protein corresponding to the apparent molecular mass of WWP1 on SDS-PAGE immunoblots of adult mouse liver and human PC3 cells. The antibody also detects lower molecular weight isoforms of WWP1 in mouse liver, and human PC3, HepG2, K562, HeLa, and HUVEC.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WWP1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for WWP1 Antibody

• AIP5
• AIP5Nedd-4-like ubiquitin-protein ligase
• atrophin-1 interacting protein 5
• Atrophin-1-interacting protein 5
• DKFZP434D2111
• EC 6.3.2
• EC 6.3.2.-
• HSDRP1
• NEDD4-like E3 ubiquitin-protein ligase WWP1
• TGIF-interacting ubiquitin ligase 1
• TIUL1
• Tiul11
• WW domain containing E3 ubiquitin protein ligase 1
• WW domain-containing protein 1
• WWP1

Background

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants; however, the full length nature of these transcripts has not been defined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WWP1