SPG20 Antibody

Images

 
Western Blot: SPG20 Antibody [NBP1-47203] - (2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: SPG20 Antibody [NBP1-47203] - (2ug/ml) staining of methanol-fixed HeLa before (left) and after (right) introduction of siRNA.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SPG20 Antibody Summary

Immunogen
Peptide with sequence C-EASGTDVKQLDQGNK corresponding to internal region according to NP_055902.1.
Epitope
C-EASGTDVKQLDQGNK
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SPG20
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:2000
  • Peptide ELISA Detection limit 1:8000
Application Notes
IF: HeLa show dimished signals after knock down of expresssion by introduction of siRNA. WB: Approx. 70 kDa band observed in HeLa lysates (calculated MW of 72.8 kDa band according to NP_055902.1).
Publications
Read Publication using NBP1-47203.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SPG20 Antibody

  • KIAA0610SPARTIN
  • spastic paraplegia 20 (Troyer syndrome)
  • Spastic paraplegia 20 protein
  • TAHCCP1spartin
  • Trans-activated by hepatitis C virus core protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SPG20 Antibody (NBP1-47203)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SPG20
Entrez
Uniprot