WWP2 Antibody

Western Blot: WWP2 Antibody [NBP1-49942] - Detection of mouse WWP2 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WWP2 antibody NBP1-49942 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western Blot: WWP2 Antibody [NBP1-49942] - Detection of human WWP2 by western blot. Samples: Whole cell lysate from HEK293T (5, 15 and 50 ug), and HeLa (50 ug), and Jurkat (50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WWP2 antibody NBP1-49942 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation: WWP2 Antibody [NBP1-49942] - Detection of human WWP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WWP2 antibody NBP1-49942 used for IP at 6 ug per reaction. WWP2 was also immunoprecipitated by another rabbit anti-WWP2 antibody. For blotting immunoprecipitated WWP2, NBP1-49942 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

WWP2 Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 200 and 250 of human WW Domain Containing E3 Ubiquitin Protein Ligase 2 using the numbering given in entry NP_008945.2 (GeneID 11060).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WWP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for WWP2 Antibody

• AIP2Nedd-4-like ubiquitin-protein ligase
• atrophin-1 interacting protein 2
• Atrophin-1-interacting protein 2
• EC 6.3.2
• EC 6.3.2.-
• NEDD4-like E3 ubiquitin-protein ligase WWP2
• WW domain containing E3 ubiquitin protein ligase 2
• WW domain-containing protein 2
• WWp2-like

Background

This gene encodes a member of the NEDD4-like protein family. The family of proteins is known to possess ubiquitin-protein ligase activity. The encoded protein contains 4 tandem WW domains. The WW domain is a protein motif consisting of 35 to 40 amino acids and is characterized by 4 conserved aromatic residues. The WW domain may mediate specific protein-protein interactions. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WWP2