WWP1 Antibody - BSA Free

Western Blot: WWP1 Antibody [NBP1-49712] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WWP1 antibody NBP1-49712 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. WWP1 was also immunoprecipitated by rabbit anti-WWP1 antibody NBP1-49713, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

WWP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit WWP1 Antibody - BSA Free (NBP1-49712) is a polyclonal antibody validated for use in WB and IP. Anti-WWP1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 200 and 250 of human WW Domain Containing E3 Ubiquitin Protein Ligase 1 using the numbering given in entry NP_008944.1 (GeneID 11059).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WWP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for WWP1 Antibody - BSA Free

• AIP5
• AIP5Nedd-4-like ubiquitin-protein ligase
• atrophin-1 interacting protein 5
• Atrophin-1-interacting protein 5
• DKFZP434D2111
• EC 6.3.2
• EC 6.3.2.-
• HSDRP1
• NEDD4-like E3 ubiquitin-protein ligase WWP1
• TGIF-interacting ubiquitin ligase 1
• TIUL1
• Tiul11
• WW domain containing E3 ubiquitin protein ligase 1
• WW domain-containing protein 1
• WWP1

Background

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants; however, the full length nature of these transcripts has not been defined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for WWP1 Antibody (NBP1-49712)(1)

Publication using NBP1-49712

• Cho EB, Yoo W, Yoon SK, Yoon JB. b-dystroglycan is regulated by a balance between WWP1-mediated degradation and protection from WWP1 by dystrophin and utrophin. Biochim. Biophys. Acta 2018-06-01 [PMID: 29635000] (WB)

Bioinformatics

• Gene Symbol: WWP1