WTAP Antibody

Images

 
Western Blot: WTAP Antibody [NBP1-31777] - Sample (30 ug of whole cell lysate) A: Molt-4 10% SDS PAGE WTAP antibody, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: WTAP Antibody [NBP1-31777] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2000.
Immunohistochemistry: WTAP Antibody [NBP1-31777] - Analysis of paraffin-embedded A549 Xenograft, using WTAP antibody at 1:100 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

WTAP Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human WTAP. The exact sequence is proprietary.
Localization
Nucleus, nucleolus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WTAP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for WTAP Antibody

  • Female-lethal(2)D homolog
  • hFL(2)D
  • KIAA0105DKFZp686F20131
  • MGC3925
  • PNAS-132
  • pre-mRNA-splicing regulator WTAP
  • putative pre-mRNA splicing regulator female-lethal(2D)
  • Wilms tumor 1 associated protein
  • Wilms tumor 1-associating protein
  • Wilms' tumour 1-associating protein
  • WT1-associated protein

Background

The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in three transcript variants, two of which encode the same isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for WTAP Antibody (NBP1-31777) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol WTAP