WT1 Antibody (WT1/857) [DyLight 550]

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Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications Flow, IHC, IHC-P
Clone
WT1/857
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 550

Order Details

WT1 Antibody (WT1/857) [DyLight 550] Summary

Immunogen
Recombinant human WT1 protein
Localization
Nuclear
Marker
Wilm's Tumor & Mesothelial Marker
Specificity
Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors.WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
WT1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-47931R in the following applications:

Reactivity Notes

Weakly reacts with Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for WT1 Antibody (WT1/857) [DyLight 550]

  • AWT1
  • GUD
  • NPHS4GUD
  • WAGR
  • Wilms tumor 1
  • Wilms tumor protein
  • WIT-2
  • WT1
  • WT33

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for WT1 Antibody (NBP2-47931R) (0)

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Review for WT1 Antibody (NBP2-47931R) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-47931R:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen WT1 NBP2-47931R
Enlarge
5
reviewed by:
DAISUKE KATAGIRI
IHC-Fr Mouse 02/03/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse kidney
SpeciesMouse
Lot7490-2PABX160926-020117R

Product General Protocols

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FAQs for WT1 Antibody (NBP2-47931R) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47931AF350
Alexa Fluor 405 NBP2-47931AF405
Alexa Fluor 488 NBP2-47931AF488
Alexa Fluor 532 NBP2-47931AF532
Alexa Fluor 594 NBP2-47931AF594
Alexa Fluor 647 NBP2-47931AF647
Alexa Fluor 700 NBP2-47931AF700
Alexa Fluor 750 NBP2-47931AF750
Allophycocyanin NBP2-47931APC
Biotin NBP2-47931B
DyLight 350 NBP2-47931UV
DyLight 405 NBP2-47931V
DyLight 488 NBP2-47931G
DyLight 550 NBP2-47931R
DyLight 594 NBP2-47931DL594
DyLight 650 NBP2-47931C
DyLight 680 NBP2-47931FR
DyLight 755 NBP2-47931IR
FITC NBP2-47931F
HRP NBP2-47931H
Janelia Fluor 549 NBP2-47931JF549
Janelia Fluor 646 NBP2-47931JF646
PE NBP2-47931PE
PerCP NBP2-47931PCP

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Bioinformatics Tool for WT1 Antibody (NBP2-47931R)

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Routine WT1 Antibody Screen Uncovers an Exciting New Cancer-Cleaning Enzyme
Enzyme antibodies are widely used in both apoptosis and cancer studies, as disruption of the proteins regulating apoptosis is known to lead to formation of tumor cells in certain cancers. We at Novus Biologicals are continually growing our enzyme anti...  Read full blog post.

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Contact Information

Learn the difference between western blot and simple western

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Calculators

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

DAISUKE KATAGIRI
02/03/2017
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol WT1