WT1 Antibody (WT1/857 + 6F-H2) - IHC-Prediluted

Images

 
Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) - IHC-Prediluted [NBP2-48223] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (WT1/857 + 6F-H2).
Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) - IHC-Prediluted [NBP2-48223] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with Wilm's TumorMonoclonal Antibody (WT1/857 + 6F-H2).
Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) - IHC-Prediluted [NBP2-48223] - Formalin-fixed, paraffin-embedded Mouse kidney stained with WT1 Monoclonal Antibody (WT1/857+6F-H2)
Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) - IHC-Prediluted [NBP2-48223] - Formalin-fixed, paraffin-embedded human Fetal kidney stained with WT1 Monoclonal Antibody (WT1/857+6F-H2).

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications IHC, IHC-P
Clone
WT1/857 + 6F-H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
IHC-Prediluted

Order Details

WT1 Antibody (WT1/857 + 6F-H2) - IHC-Prediluted Summary

Immunogen
Recombinant full length human WT1 protein (WT1/857); Recombinant fragment aa1-181 of human WT1 (6F-H2)
Localization
Nuclear
Marker
Wilm's Tumor & Mesothelial Marker
Specificity
Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors.WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
WT1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Hu-chromosome location: 11p13
Molecular weight of antigen: 47-55kDa
Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT,Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Alternate Names for WT1 Antibody (WT1/857 + 6F-H2) - IHC-Prediluted

  • AWT1
  • GUD
  • NPHS4GUD
  • WAGR
  • Wilms tumor 1
  • Wilms tumor protein
  • WIT-2
  • WT1
  • WT33

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for WT1 Antibody (NBP2-48223) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47858AF350
Alexa Fluor 405 NBP2-47858AF405
Alexa Fluor 488 NBP2-47858AF488
Alexa Fluor 532 NBP2-47858AF532
Alexa Fluor 594 NBP2-47858AF594
Alexa Fluor 647 NBP2-47858AF647
Alexa Fluor 700 NBP2-47858AF700
Alexa Fluor 750 NBP2-47858AF750
Allophycocyanin NBP2-47858APC
Biotin NBP2-47858B
DyLight 350 NBP2-47858UV
DyLight 405 NBP2-47858V
DyLight 488 NBP2-47858G
DyLight 550 NBP2-47858R
DyLight 594 NBP2-47858DL594
DyLight 650 NBP2-47858C
DyLight 680 NBP2-47858FR
DyLight 755 NBP2-47858IR
FITC NBP2-47858F
HRP NBP2-47858H
Janelia Fluor 549 NBP2-47858JF549
Janelia Fluor 646 NBP2-47858JF646
PE NBP2-47858PE
PerCP NBP2-47858PCP

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Bioinformatics

Gene Symbol WT1