WT1 Antibody (WT1/857 + 6F-H2)

Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (WT1/857 + 6F-H2).

Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded Rat kidney stained with WT1 monoclonal antibody (WT1/857+6F-H2).

Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded human Fetal kidney stained with WT1 Monoclonal Antibody (WT1/857+6F-H2).

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clone: WT1/857 + 6F-H2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

WT1 Antibody (WT1/857 + 6F-H2) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47858)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant full length human WT1 protein (WT1/857); Recombinant fragment aa1-181 of human WT1 (6F-H2) (Uniprot: P19544)
• Localization: Nuclear and Cytoplasmic
• Marker: Wilm's Tumor & Mesothelial Marker
• Specificity: Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilms tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
• Isotype: IgG1 Kappa/IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: WT1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33631230).

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for WT1 Antibody (WT1/857 + 6F-H2)

• AWT1
• GUD
• NPHS4GUD
• WAGR
• Wilms tumor 1
• Wilms tumor protein
• WIT-2
• WT1
• WT33

Background

Wilms' tumor protein (WT1) is a transcription factor which regulates the expression of numerous target genes, including EPO. This protein plays an essential role in the normal development of the urogenital system, including cellular development and cell survival, and it has both tumor suppressor and oncogenic roles in tumor formation. Defects in the WT1 protein can lead to Wilms tumor, Frasier syndrome, Denys-Drash syndrome, nephrotic syndrome type 4, and Meacham syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for WT1 Antibody (NBP2-44607)(8)

Publications using NBP2-44607

• Jillian Williquett, Chantal Allamargot, Hua Sun AMPK-SP1–Guided Dynein Expression Represents a New Energy-Responsive Mechanism and Therapeutic Target for Diabetic Nephropathy Kidney360 2024-04-01 [PMID: 38467599]
• Wang Z, Wu Q, Wang H et al. Diosgenin protects against podocyte injury in early phase of diabetic nephropathy through regulating SIRT6 Phytomedicine : international journal of phytotherapy and phytopharmacology 2022-06-13 [PMID: 35728388] (IF/IHC, Mouse)
• Yang X, Luo W, Li L et al. CDK9 inhibition improves diabetic nephropathy by reducing inflammation in the kidneys Toxicology and applied pharmacology 2021-02-22 [PMID: 33631230] (IHC-P, Mouse)
• Luo W, Chen X, Ye L et al. Kaempferol attenuates streptozotocin-induced diabetic nephropathy by downregulating TRAF6 expression: the role of TRAF6 in diabetic nephropathy Journal of Ethnopharmacology 2020-11-01 [PMID: 33152432] (IHC-P, Mouse)
• Chen X, Zhang X, Xu J et Al. AZD4547 Attenuates Lipopolysaccharide-Induced Acute Kidney Injury by Inhibiting Inflammation: The Role of FGFR1 in Renal Tubular Epithelial Cells Drug Des Devel Ther 2020-02-26 [PMID: 32161443] (IHC-P, IF/IHC, Mouse)
• Chen X, Zhao Y, Xu J et al. The Nephroprotective Effect of TNF Receptor-Associated Factor 6 (TRAF6) Blockade on LPS-Induced Acute Renal Injury Through the Inhibition if Inflammation and Oxidative Stress Med. Sci. Monit. 2020-01-13 [PMID: 31929494] (IHC-P, Mouse)
• Zheng C, Huang L et al. Inhibition of STAT3 in tubular epithelial cells prevents kidney fibrosis and nephropathy in STZ-induced diabetic mice. Cell Death Dis 2019-07-11 [PMID: 31699972] (IF/IHC, Mouse)
• Liang G, Song L et al. Fibroblast growth factor 1 ameliorates diabetic nephropathy by an anti-inflammatory mechanism. Kidney Int 2018-01-01 [PMID: 28750927] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: WT1
• Uniprot:
  • P19544(Human)