WT1 Antibody (rWT1/6908) - Azide and BSA Free

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with WT1 Antibody (rWT1/6908) - Azide and BSA Free. Inset: PBS instead of primary antibody; secondary only negative control.

• Reactivity: Hu
• Applications: IHC
• Clone: rWT1/6908
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

WT1 Antibody (rWT1/6908) - Azide and BSA Free Summary

• Description: Prepared in 10mM PBS WITHOUT BSA & azide at 1.0mg/ml. Also available with 0.05% BSA & 0.05% azide.
  
  Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment (around aa1-100) of human WT1 protein (exact sequence is proprietary) (Uniprot: P19544)
• Localization: Cytoplasm, Nucleolus, Nucleoplasm, Nucleus, Nucleus speckle
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: WT1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Alternate Names for WT1 Antibody (rWT1/6908) - Azide and BSA Free

• AWT1
• GUD
• NPHS4GUD
• WAGR
• Wilms tumor 1
• Wilms tumor protein
• WIT-2
• WT1
• WT33

Background

Wilms' tumor protein (WT1) is a transcription factor which regulates the expression of numerous target genes, including EPO. This protein plays an essential role in the normal development of the urogenital system, including cellular development and cell survival, and it has both tumor suppressor and oncogenic roles in tumor formation.

Defects in the WT1 protein can lead to Wilms tumor, Frasier syndrome, Denys-Drash syndrome, nephrotic syndrome type 4, and Meacham syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WT1