Wnt-7a/b Antibody [Unconjugated]

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Wnt‑7a/b was detected in immersion fixed paraffin-embedded sections of human endometrial cancer tissue using 15 µg/mL Goat Anti-HumanWnt‑7a/b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3008) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Wnt-7a/b Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Wnt‑7a
Leu32-Glu72, Thr216-Arg292
Accession # O00755
Specificity
Detects human Wnt‑7a and Wnt-7b in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Wnt-1, rmWnt-3a, rmWnt-4, rmWnt-5a, rmWnt-5b, rmWnt-9a, rmWnt-11, and rmWnt‑16 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF3008 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-7a/b Antibody [Unconjugated]

  • Wnt-7a
  • Wnt-7a/b

Background

Human Wnt-7a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1). Wnts bind to receptors of the Frizzled family in conjunction with low-density lipoprotein receptor‑related proteins (LRPs). Downstream effects of Wnt signaling occur through activation of one of three different intracellular pathways: the canonical Wnt pathway, Wnt/Ca2+ pathway, and planar cell polarity. The highly transforming Wnts, including Wnt-1, -3, -3a, -7a and -8 activate the canonical pathway, which regulates beta ‑catenin-mediated gene expression (1, 2). Human Wnt‑7a is a 48 kDa secreted glycoprotein containing 24 cysteine residues that is expressed by epithelial and epithelially-derived cells of the placenta, kidney, testis, uterus, fetal lung, and fetal and adult brain (3, 4). Palmitate modification of a cysteine residue has been shown for Wnt‑3a; this site is conserved on all Wnts and is Cys73 on Wnt‑7a (5). When modified, increased hydrophobicity and activity is expected. Human Wnt‑7a shows 97% aa identity with mouse, rat, and dog Wnt‑7a and 92% aa identity with chicken Wnt‑7a. During development, Wnt‑7a is expressed by the dorsal ectoderm and drives expression of homeodomain transcription factors that control effectors important in patterning and cell fates in adjacent mesenchyme (6-10). When Wnt‑7a is deleted, mice show disruption of dorsalization and anterior/posterior patterning during limb development and abnormalities in the reproductive tract (6-10). Wnt‑7a is frequently downregulated in leukemia and lung cancers, potentially affecting homeobox (HOX) gene expression, differentiation state and growth control (11, 12). Roles for Wnt‑7a have also been shown during formation of neural synapses, response of the uterus to estrogen and inflammatory cartilage destruction (10, 13, 14). Wnt-7b is a 46 kDa, secreted glycosylated protein that belongs to the Wnt family. Wnt proteins can be lipid-modified and are ligands for members of the frizzled family of receptors, which mediates cell-cell communication during development. Human Wnt-7b is synthesized as a 349 amino acid (aa) precursor that contains a 318 aa mature region. The mature region contains 24 cysteines and three potential N-linked glycosylation sites. Wnt-7b is a ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Mature human Wnt-7b shares 99% aa sequence identity with mature mouse and rat Wnt-7b. Wnt-7b also shares 80% aa sequence identity with Wnt-7a.

  1. Logan, C.Y. and R. Nusse (2004) Annu. Rev. Cell Dev. Biol. 20:781.
  2. Wong, G. T. et al. (1994) Mol. Cell. Biol. 14:6278.
  3. http://www.stanford.edu/~rnusse/wntwindow.html 
  4. Bui, T. D. et al. (1997) Gene 189:25.
  5. Willert, K. et al. (2003) Nature 423:448.
  6. Parr, B.A. and A.P. McMahon (1995) Nature 374:350.
  7. Cygan, J.A. et al. (1997) Development 124:5021.
  8. Parr, B.A. and A.P. McMahon (1998) Nature 395:707.
  9. Miller, C. and D. Sassoon (1998) Development 125:3201.
  10. Carta, L. and D. Sassoon (2004) Biol. Reprod. 71:444.
  11. Calvo, R. et al. (2000) Proc. Natl. Acad. Sci. USA 97:12776.
  12. Winn, R.A. et al. (2005) J. Biol Chem. 280:19625.
  13. Hall, A.C. et al. (2000) Cell 100:525.
  14. Hwang, S-G. et al. (2004) J. Biol. Chem 279:26597.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Wnt-7a/b Antibody (AF3008)(4)

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