Wnt-3a Antibody (930769) [Unconjugated]

Recombinant Mouse Wnt-3a (Catalog # 1324-WN) induces Topflash reporter activity in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line), as measured by the Topflash Reporter Assay. Under these conditions, Topflash reporter activity elicited by Recombinant Mouse Wnt-3a (0.5 μg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human/Mouse Wnt-3a Monoclonal Antibody (Catalog # MAB9025). The ND50 is typically0.15‑0.9 μg/mL.

• Reactivity: Hu, Mu
• Applications: B/N
• Clone: 930769
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Wnt-3a Antibody (930769) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Wnt-3a
  Ser19-Lys352
  Accession # P27467
• Specificity: Detects human and mouse Wnt-3a in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.15-0.9 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-3a Antibody (930769) [Unconjugated]

• MGC119418
• MGC119419
• MGC119420
• protein Wnt-3a
• wingless-type MMTV integration site family, member 3A
• Wnt3a
• Wnt-3a

Background

Wnt-3a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1‑3). Wnts bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase (1, 4). Mouse Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues (5). Like other Wnts, Wnt-3a is modified by palmitate addition (at Cys 77) following glycosylation, which increases its hydrophobicity, secretion and activity (6, 7). A second site at Ser 209 is modified by palmitoleic acid and also contributes to activity and secretion (8). Mouse Wnt-3a shares 96% amino acid (aa) identity with human Wnt-3a, and 97% with bovine and canine Wnt-3a. The rat Wnt-3a precursor as it is apparently translated shares 100% aa identity with mouse Wnt-3a aa 63-352 (9). Wnt-3a also shares 87% aa identity with Wnt-3. During development, Wnt-3a is morphogen that is thought to coordinate somitogenesis and mesoderm boundary determination, and is expressed at the same locations and times as Wnt-2b and Wnt-5a (10). When Wnt-3a is deleted, mice fail to develop a hippocampus, and show defects in anterior-posterior patterning, somite development and tailbud formation (10-13). Recombinant Wnt-3a promotes proliferation of committed stem cell lineages in vitro, and may help maintain the cells in an undifferentiated state (6, 14) For example, Wnt-3a can induce self-renewal of hematopoietic stem cells, allowing expansion without further differentiation (6).

1. Mikels, A.J. and R. Nusse (2006) Oncogene 25:7461.
2. Miller, J.R. (2001) Genome Biol. 3:3001.
3. Roelink, H. and R. Nusse (1991) Genes Dev. 5:381.
4. Lu, W et al. (2004) Cell 119:97.
5. Smolich, B.D. et al. (1993) Mol. Biol Cell 4:1267.
6. Willert, K. et al. (2003) Nature 423:448.
7. Komekado, H. et al. (2007) Genes Cells 12:521.
8. Takada, R. et al. (2006) Dev. Cell 11:791.
9. Entrez Accession # NP_001100475.
10. Dunty, W.C. et al. (2008) Development 135:85.
11. Lee, S.M.K. et al. (2000) Development 127:457.
12. Takada, S. et al. (1994) Genes Dev. 8:174.
13. Ikeya, M. and S. Takada (2001) Mech. Dev. 103:27.
14. Nusse, R. (2008) Cell Res. 18:523.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Wnt-3a Antibody (MAB9025)(6)

Publications using MAB9025

• YC Hsueh, RE Pratt, VJ Dzau, CP Hodgkinson Novel method of differentiating human induced pluripotent stem cells to mature cardiomyocytes via Sfrp2 Scientific Reports, 2023-03-09;13(1):3920. 2023-03-09 [PMID: 36894665] (Neutralization, Human)
• GFA de Moraes, E Listik, GZ Justo, CM Vicente, L Toma The Glypican proteoglycans show intrinsic interactions with Wnt-3a in human prostate cancer cells that are not always associated with cascade activation BMC molecular and cell biology, 2021-05-04;22(1):26. 2021-05-04 [PMID: 33947326] (Flow Cytometry, IHC, Immunoprecipitation, Western Blot, Human)
• A Weinstock, K Rahman, O Yaacov, H Nishi, P Menon, CA Nikain, ML Garabedian, S Pena, N Akbar, BE Sansbury, SP Heffron, J Liu, G Marecki, D Fernandez, EJ Brown, KV Ruggles, SA Ramsey, C Giannarell, M Spite, RP Choudhury, P Loke, EA Fisher Wnt signaling enhances macrophage responses to IL-4 and promotes resolution of atherosclerosis Elife, 2021-03-15;10(0):. 2021-03-15 [PMID: 33720008] (Neutralization, Mouse)
• Sohinee Bhattacharyya, Chet Oon, Aayush Kothari, Wesley Horton, Jason Link, Rosalie C. Sears et al. Acidic fibroblast growth factor underlies microenvironmental regulation of MYC in pancreatic cancer Journal of Experimental Medicine 2020-08-03 [PMID: 32434218] (Neutralization, Human)
• Tomomi M. Yamamoto, Alexandra McMellen, Zachary L. Watson, Jennifer Aguilera, Rebecca Ferguson, Elmar Nurmemmedov et al. Activation of Wnt signaling promotes olaparib resistant ovarian cancer Molecular Carcinogenesis 10/1/2019 [PMID: 31219654]
• R El Helou, G Pinna, O Cabaud, J Wicinski, R Bhajun, L Guyon, C Rioualen, P Finetti, A Gros, B Mari, P Barbry, F Bertucci, G Bidaut, A Harel-Bell, D Birnbaum, E Charafe-Ja, C Ginestier miR-600 Acts as a Bimodal Switch that Regulates Breast Cancer Stem Cell Fate through WNT Signaling Cell Rep, 2017-02-28;18(9):2256-2268. 2017-02-28 [PMID: 28249169] (Neutralization, Human)

Bioinformatics

• Uniprot:
  • P27467()