Wnt-1 Antibody (13F9)

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Western Blot: Wnt-1 Antibody (13F9) [NBP1-42739] - Analysis of Wnt1 protein in mouse testis lysate. The results show specific binding corresponding to the 41 kDa Wnt1 protein. Primary antibody was used at a 1:500 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
13F9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Wnt-1 Antibody (13F9) Summary

Immunogen
Synthetic peptide corresponding to an internal region of human Wnt1 protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
WNT1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:175-1:250
  • ELISA 1:4000-1:20000
Application Notes
This protein-A purified antibody has been tested for use western blotting. Expect a band approximately 41 kDa in size corresponding - Wnt1 by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Protein A purified

Alternate Names for Wnt-1 Antibody (13F9)

  • Int-1
  • INT1Proto-oncogene Int-1 homolog
  • proto-oncogene Wnt-1
  • wingless-type MMTV integration site family, member 1 (oncogene INT1)
  • wingless-type MMTV integration site family, member 1
  • Wnt1
  • Wnt-1

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt1 (Wingless-type MMTV integration site family member 1) is a member of the WNT gene family. It is highly conserved in evolution and the protein encoded by this gene is known to be 98% identical to mouse Wnt1 protein at the amino acid level. Studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. Wnt1 is secreted as an extracellular matrix protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol WNT1
Entrez