WIZ Antibody - BSA Free

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Immunocytochemistry/Immunofluorescence: WIZ Antibody [NBP1-80586] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: WIZ Antibody [NBP1-80586] - Staining of human pancreas shows strong nuclear and slightly weaker nuclear positivity in exocrine as well as endocrine cells.

The staining of human testis shows strong nuclear-cytoplasmic positivity in cells in seminiferous ducts.

Staining of human duodenum shows strong nuclear-cytoplasmic positivity in glandular cells and lymphoid cells.

Staining of human skin shows strong nuclear-cytoplasmic positivity in squamous epithelial cells.

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WIZ-Antibody-Western-Blot-NBP1-80586-img0005.jpg

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

WIZ Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit WIZ Antibody - BSA Free (NBP1-80586) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and ChIP. Anti-WIZ Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SPSDLHISPLAKKLPPPPGSPLGHSPTASPPPTARKMFPGLAAPSLPKKLKPEQIRVEIKREMLPGALHGELHPSEGPWGAPREDMTPLNLSSR
• Predicted Species: Rat (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: WIZ
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) Application provided by CiteAb from the following publication (https://elifesciences.org/articles/15082),
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot Application provided by CiteAb from the following publication (https://elifesciences.org/articles/15082),
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
• Control Peptide:

  WIZ Protein (NBP1-80586PEP)

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32294452).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for WIZ Antibody - BSA Free

• widely interspaced zinc finger motifs
• widely-interspaced zinc finger motifs
• Widely-interspaced zinc finger-containing protein
• WIZ zinc finger
• WIZ
• Zinc finger protein 803
• ZNF803
• ZNF803protein Wiz

Background

WIZ may link EHMT1 and EHMT2 histone methyltransferases to the CTBP corepressor machinery. May be involved inEHMT1-EHMT2 heterodimer formation and stabilization

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for WIZ Antibody (NBP1-80586)(7)

Publications using NBP1-80586

• Yelagandula R, Stecher K, Novatchkova M et al. ZFP462 safeguards neural lineage specification by targeting G9A/GLP-mediated heterochromatin to silence enhancers Nature cell biology 2023-01-01 [PMID: 36604593] (ChIP)
  
  Details:
  Chromatin from human embryonic stem cells (hESCs) was spiked into mouse ESC chromatin, 1% relative to mouse chromatin
• Whitley SK, Li M, Kashem SW et al. Local IL-23 is required for proliferation and retention of skin-resident memory TH17 cells Science immunology 2022-11-18 [PMID: 36367947]
• Ball DP, Tsamouri LP, Wang AE et al. Oxidized thioredoxin-1 restrains the NLRP1 inflammasome Science immunology 2022-11-11 [PMID: 36332009] (Western Blot)
• Chan KL, Gomez J, Cardinez C, Kumari N et Al. Inhibition of the CtBP complex and FBXO11 enhances MHC class II expression and anti-cancer immune responses Cancer Cell 2022-09-30 [PMID: 36179686]
• Bukova I, Szczerkowska KI, Prochazkova M et al. Loss of Wiz Function Affects Methylation Pattern in Palate Development and Leads to Cleft Palate Frontiers in cell and developmental biology 2021-06-02 [PMID: 34150743] (IF/IHC, Mouse)
• Justice M, Carico ZM, Stefan HC, Dowen JM A WIZ/Cohesin/CTCF Complex Anchors DNA Loops to Define Gene Expression and Cell Identity Cell Rep 2020-04-14 [PMID: 32294452] (Chemotaxis, Human, Mouse)
• Isbel L, Prokopuk L, Wu H et al. Wiz binds active promoters and CTCF-binding sites and is required for normal behaviour in the mouse Elife 2016-07-13 [PMID: 27410475] (WB)

FAQs for WIZ Antibody (NBP1-80586). (Showing 1 - 1 of 1 FAQs).

1. What is the concentration for the lot# A96963 of this antibody?
   • The concentration of Lot: A96963 is 0.2mg/ml.

Bioinformatics

• Gene Symbol: WIZ
• Entrez:
  • 58525(Human)
• Uniprot:
  • O95785(Mouse)