WIF-1 Antibody (1G5)

Images

 
Western Blot: WIF-1 Antibody (1G5) [NBP1-51658] - Western blot analysis using WIF1 mouse mAb against Hela (1), NIH/3T3 (2) and NTERA-2 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: WIF-1 Antibody (1G5) [NBP1-51658] - Immunofluorescence analysis of Hela cells using WIF1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: WIF-1 Antibody (1G5) [NBP1-51658] - Immunohistochemical analysis of paraffin-embedded ovary tumour tissues (left) and lung cancer (right) using WIF1 mouse mAb with DAB staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
1G5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

WIF-1 Antibody (1G5) Summary

Immunogen
Purified recombinant fragment of human WIF1 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
WIF1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for WIF-1 Antibody (1G5)

  • WIF1
  • WIF-1
  • WNT inhibitory factor 1

Background

WNT proteins are extracellular signaling molecules involved in the control of embryonic development. This gene encodes a secreted protein, which binds WNT proteins and inhibits their activities. This protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF)-like domains. It may be involved in mesoderm segmentation. This protein is found to be present in fish, amphibia and mammals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for WIF-1 Antibody (NBP1-51658) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol WIF1
Entrez